VWR CO2 Incubator Basic User Manual
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VWR CO2 Incubator Basic User Manual

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VWR CO2 Incubator Basic
50150229
Revision 0
December 2015

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Summary of Contents for VWR CO2 Incubator Basic

  • Page 1 VWR CO2 Incubator Basic 50150229 Revision 0 December 2015 ...
  • Page 2 © 2015 VWR International, LLC. All rights reserved. This Instruction Manual is copyright protected. Rights resulting thereof, particularly reprint, photomechanical or digital post-processing or reproduction, even in part, are only allowed with the written consent of VWR International, LLC. This regulation does not apply to reproductions for in-plant use.

  • Page 3: Table Of Contents

    Table of Contents Chapter 1 Important safety information ..........1-3 Instruction of the operating personnel.
  • Page 4 Operating panel ............7-42 Control self-test .

  • Page 5: Chapter 1 Important Safety Information

    Keep these operating instructions close to the device so that safety instructions and  important information are always accessible. Should you encounter problems that are not detailed adequately in these operating  instructions, please contact VWR immediately for your own safety. CO2 Incubator Basic | 3...

  • Page 6: Warranty

     described in these operating instructions The device is not modified  Only original spare parts and accessories that have been approved by VWR are used  Inspections and maintenance are performed at the specified intervals  The warranty is valid from the date of delivery of the device to the operator.
  • Page 7 Safety signs, test marks and marks of conformity placed on the device. Observe operating instructions! CSA test mark: Test certificate for USA/Canada, indicates that the product has met the requirements of nationally recognized product safety standards. CO2 Incubator Basic | 5...

  • Page 8: Overview Safety Information

    Pressure compensation opening: To ensure permanent pressure compensation, the pressure compensation opening must not be connected to an exhaust air system. The pipe of the pressure compensation opening must not be extended or redirected! CO2 Incubator Basic...
  • Page 9 Hygiene regulations: Prior to any operation, the user must clean and disinfect the work space in accordance with the hygiene regulations set forth by the operator to protect the cultures. CO2 Incubator Basic | 7...
  • Page 10 Demineralized and distilled or autoclaved for sterilization  Completely deionized and distilled or autoclaved for sterilization  When the water level falls below the lower limit, refill water!  CO2 Incubator Basic...

  • Page 11: Intended Purpose Of The Device

    L1, L2, and L3. Do not use tissues, substances or liquids that: Are easily ignitable or explosive  Release vapors that form combustible or explosive mixtures when exposed to air  Release poisons  CO2 Incubator Basic | 9...
  • Page 12 230 V supply and 1500 V for a 120 V supply. Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2 assumes that normally only non-conductive pollution such a dust occurs with the exception of occasional conductivity caused by condensation. CO2 Incubator Basic...

  • Page 13: Chapter 2 Delivery

    Connector, potential-free contact Spare caps, set connecting hose set Immersion water pump Open-end wrench, 24 mm Allen wrench 2 mm for blower wheel Allen wrench 3 mm for blower wheel cover Operating instructions Pump for water draining CO2 Incubator Basic | 11...

  • Page 14: Acceptance Inspection

    Chapter 2 | Delivery Acceptance inspection After the device has been delivered, check the delivery immediately for: Completeness  Possible damages  If damages are detected or if components are missing, please contact the carrier and vendor immediately. CO2 Incubator Basic...

  • Page 15: Chapter 3 Installation Of The Device

    In addition, heat dissipating from the device during permanent operation may cause a change in the room climate. CO2 Incubator Basic | 13...

  • Page 16: Space Requirements

    The height of the floor stand should not be less than 200 mm. Several floor stands and carriers are available as options from VWR (Part no. see Chapter 11 “Parts and accessories”).

  • Page 17: Transport

    For transport do not lift the device using the doors or components attached to the device (e.g. control box on rear panel) as lift points. NOTE! Lift points: Lift the device only using the lift points shown in Figure 2. CO2 Incubator Basic | 15...

  • Page 18: Stacking

    Transporting stacked devices: The stacking elements are not connecting elements. Therefore, the transport of stacked devices on sloped surfaces is not allowed! Modifications NOTE! Modifications: Retrofits and modifications must only be performed by the Technical Service of VWR! CO2 Incubator Basic...

  • Page 19: Chapter 4 Description Of The Device

    An independent thermal protection protects the samples from harmful overheating in the  event of a failure A pressure compensation opening ensures pressure compensation in the device work space  Audible and visual alarms indicate failures during operation  CO2 Incubator Basic | 17...

  • Page 20: Views Of Device

    Figure 4 – General views of device Front view: [1] Stacking elements [2] Plug caps [3] Glass door [4] Measuring cell with blower wheel and sensors [5] Door switch [6] Pressure compensation opening with insert [7] Measuring opening | CO2 Incubator Basic...
  • Page 21 [17] Support hook for shelf [18] Access port with plug [19] Glass door seal, replaceable Rear view [20] Stacking elements [21] Pressure compensation opening [22] Access port, Ø 42 mm [23] Switchbox with supply interfaces [24] CO gas container CO2 Incubator Basic | 19...

  • Page 22: Work Space Atmosphere

    The CO content of the work space atmosphere can be controlled within a range of 0 - 20%. The CO must be of the following quality: Purity 99.5% min  Medical grade  | CO2 Incubator Basic...

  • Page 23: Sensor System

    Any response of the thermal protection will simultaneously trigger an audible and visual alarm. CO2 Incubator Basic | 21...

  • Page 24: Door Switch

    If the door remains open for more than 30 seconds, a short acoustic alarm sounds. If the door remains open for more than 10 minutes, the acoustic alarm sounds continuously. The outer door can only be closed after the glass door has been latched properly. | CO2 Incubator Basic...

  • Page 25: Switchbox With Supply Interface

    Description of the device | Chapter 4 Switchbox with supply interface Figure 7 – Supply interfaces CO2 Incubator Basic | 23...
  • Page 26 Power supply connection: The power supply connection [5] of the device is established via a cable with a connector for non heating appliances. The holder for the two device fuses is integral to the power supply socket. | CO2 Incubator Basic...

  • Page 27: Work Space Components

    Description of the device | Chapter 4 Work space components Figure 8 – Water space layout / Water reservoir CO2 Incubator Basic | 25...
  • Page 28 The support rails [1] of the shelf system are perforated every 42 mm so that the support hooks [8] can be inserted variably for any culture container size required. The shelves [2] have an integrated tilt protection and withdrawal stop. For details of the shelf system, see Chapter 5 “Start-up”. | CO2 Incubator Basic...
  • Page 29 24 °C RT + 6.5 °C 27.5 °C 10 W RT + 9.5 °C 30.5 °C 15 W RT + 13 °C 34 °C 20 W RT + 16 °C 37 °C *RT = Ambient temperature CO2 Incubator Basic | 27...

  • Page 30: C Decontamination Routine (Optional)

    9 hours at a temperature of 90° C. The effectiveness of the decontamination routine has been tested and certified by independent institutes. Information about these tests is available at request from VWR. The entire program run of the decontamination routine will take approx. 25 hours.

  • Page 31: Chapter 5 Start-Up

    Tools are not required for the installation of the shelf system. The support rails are secured using spring pressure. After the support hooks have been inserted into the rail, the shelves are pushed onto the support hooks. CO2 Incubator Basic | 29...
  • Page 32 The support rails are held at the sides by embossing [2] and [5] and secured by the embossing [1] and [6]. The support rails marked with ( ) are inserted at the rear panel of the device with the locksprings [3] facing upward. CO2 Incubator Basic...
  • Page 33 2. Slightly raise shelf so that the withdrawal stop [6] can be routed over the shelf supports. 3. Make sure that the shelf supports are positioned in the two tilt protectors in a way that it can move freely. CO2 Incubator Basic | 31...

  • Page 34: Gas Connection

    To ensure permanent pressure compensation, the pressure compensation opening must not be connected to an exhaust air system. The pipe of the pressure compensation opening must not be extended or redirected! Access port: If the access port is not used, it must be capped during operation! CO2 Incubator Basic...

  • Page 35: Power Supply Connection

    Do not use damaged components when connecting the device to the power supply! The device must be connected only to a correctly installed and grounded power supply source: Fusing: T 16 A  Circuit breaker: G 16  CO2 Incubator Basic | 33...
  • Page 36 2. Connect the connector for non-heating appliances [2] to the socket [1] at the control box of the device. 3. Connect the grounding plug [3] of the power supply cable to a correctly grounded and fused socket. 4. Make sure the power supply cable is not subjected to tensile or compressive force. CO2 Incubator Basic...

  • Page 37: Rs 232 Interface Connection

    (command XOR data XOR ... XOR data N XOR FF Command list - Reading control loop data 0110 0001 (61 Data: 0001 0000 (10 ) for temperature during incubation operation 0001 0001 (11 ) for CO CO2 Incubator Basic | 35...
  • Page 38 <Lowbyte> in the memory. For the PC, this sequence is reversed. The microcontroller transmits these values in its format, i.e. the PC must reverse the sequence of the bytes. For floats, there is no difference. Example: Temperature data request and response CO2 Incubator Basic...

  • Page 39: Connecting The Alarm Contact

    Connecting the alarm contact NOTICE! Expert work: VWR warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately...
  • Page 40 1. Connect the individual conductors [1] to [4] of the connecting cable as shown in the wiring diagram. 2. Connect the connector [5] of the alarm system connecting cable to the interface [6] at the control box at the rear panel of the device. CO2 Incubator Basic...

  • Page 41: Chapter 6 Operation

    Demineralized and distilled or autoclaved for sterilization  Completely deionized and distilled or autoclaved for sterilization  When the water level falls below the lower limit, refill water! CO2 Incubator Basic | 39...

  • Page 42: Starting Operation

    To ensure sufficient air circulation and even heating of the samples, the loading surface within the work space should be used up to max. 70%. Large objects in the work space that dissipate heat may impair heat distribution! CO2 Incubator Basic...

  • Page 43: Chapter 7 Handling And Control

    Handling and control Power switch Figure 16 – Power switch Depending on which side the door hinges are installed, the power switch is integral to the front cover of one of the front device stands. [1] Power Switch [2] Front cover To turn the device on: Press the power switch [1];...

  • Page 44: Operating Panel

    [12] LED for indicating active optional 90 °C decontamination routine [13] Key for starting optional decontamination routine [14] Key for starting cal function [15] Key for reducing value [16] Key for setting CO nominal value [18] CO display CO2 Incubator Basic...

  • Page 45: Control Self-Test

    The temperature display shows the current temperature value, the CO display shows the current CO value. NOTE! Factory pre-settings: Upon delivery of the device, the following nominal values have been set: Temperature: 37 °C  content: 0.0%  CO2 Incubator Basic | 43...

  • Page 46: Setting The Nominal Temperature Value

    UP/DOWN function switches to a rapid increase/reduction; after approx. 3 seconds, another increase/reduction occurs. Press the keys. To reduce the nominal value: Press the keys. 3. Accept and store the nominal value: Release both keys. The CO display shows the current nominal value measured in the work space. CO2 Incubator Basic...

  • Page 47: Activating The Auto-Start Routine

    3. Activate the auto-start routine: Keep the key depressed for 5 seconds. The auto-start LED flashes. 4. Close all device doors: The temperature display shows the actual value, the CO display shows “run”, the “door” LED goes off. CO2 Incubator Basic | 45...

  • Page 48: Reading Failure Codes

    After the glass door has been opened: max. 45 min  Other failure causes: max. 1 min!  Delay time reset: If the set nominal value is reached during the specified period, the delay time is reset to 1 min! CO2 Incubator Basic...
  • Page 49 For a list and a description of the failure codes, please refer to failure code list in this section! 4. Scroll through the failure code table: To read stored failure codes from the failure code table. Keep the key depressed. To scroll, keep the key depressed. CO2 Incubator Basic | 47...
  • Page 50 The temperature and CO displays show the current actual values. 6. Erase the failure code table: Keep the depressed for 5 seconds. The temperature and CO displays flash to indicate that the failure table has been erased. CO2 Incubator Basic...

  • Page 51: Failure Code List

    Actual value > 85°C Contact service tine above nominal value Failure in 90°C routine Power failure during heat- Silence alarm by pressing key ing or holding phase 90°C (2 times 5 sec.), then restart routine CO2 Incubator Basic | 49...

  • Page 52: Resetting The Thermal Protection

    (e.g. by ventilating the room or by reducing the temperature in the operating room), the thermal protection will respond again immediately; in this case, contact Technical Service! CO2 Incubator Basic...

  • Page 53: Chapter 8 Shut-Down

    6. Disconnect gas pressure hoses from sleeve at the rear of the device. 7. Until the device is shut down, the work space must be continuously ventilated: Leave the glass door and the outer door open and secure them in this state. CO2 Incubator Basic | 51...
  • Page 54 Chapter 8 | Shut-down CO2 Incubator Basic...

  • Page 55: Chapter 9 Cleaning And Disinfection

    Do not use sodiumhypochlorite solutions such as Purex and Clorox. These can cause  corrosion and pitting of stainless steel. Do not use steel wood pads such as Brillo. These pads deposit carbon particles in the work  space. CO2 Incubator Basic | 53...
  • Page 56 Observe safety regulations to avoid fire and/or explosion hazard caused by alcohol-containing  disinfectants! Recommended disinfectant: A surface disinfectant recommended by VWR can be ordered under following part numbers: Spray bottle, 250 ml: Part No.: 50052425  Refill bottle, 500 ml: Part No.: 50051939 ...
  • Page 57 For removal and installation of the shelf system, please refer to Chapter 5, “Start-up”. 2. If required, remove the blower wheel and its cover from the baseplate of the measuring cell. The wheel and the cover can be autoclaved. CO2 Incubator Basic | 55...
  • Page 58 1. Install shelf system and accessories. 2. Again, spray disinfectant onto the surfaces of the work space and of the accessories or wipe the surfaces clean using disinfectant. 3. Allow disinfectant to react as specified by manufacturer. CO2 Incubator Basic...

  • Page 59: C Decontamination Routine (Optional)

    To cancel the routine completely, the operating step must be executed repeatedly until the remaining run time display shows the value 0. When the glass door is opened, this operating step cancels the routine completely. CO2 Incubator Basic | 57...
  • Page 60 5 seconds. The 90 °C LED flashes. Close the device doors: The actual value is shown at the temperature display. The remaining run time is shown at the CO display. The door LED goes off. CO2 Incubator Basic...
  • Page 61 To completely cancel the routine, each phase must be skipped individually by pressing the key until a remaining run time of 0 hours is displayed. When the last phase (postheating phase) has been skipped, the routine is completely cancelled. CO2 Incubator Basic | 59...
  • Page 62 Chapter 9 | Cleaning and disinfection CO2 Incubator Basic...

  • Page 63: Chapter 10 Maintenance

    During normal operation, the following service routine must be performed: Weekly service: Refill the work space water tray with fresh processed water  3-month service: Run auto-start routine  Perform temperature and CO calibration  CO2 Incubator Basic | 61...

  • Page 64: Preparing The Temperature Calibration

     NOTE! Service contract: VWR offer a device-specific service contract that comprises all test and service works required! Preparing the temperature calibration To determine the exact measured value of the integrated temperature sensor, a temperature comparison measurement has to be performed every three months.
  • Page 65 [1] at the rear panel of the device. 4. Close doors. 5. Wait until the temperature value displayed on the measuring instrument has stabilized. 6. Calibrate temperature control as described in Section “Temperature calibration procedure”. CO2 Incubator Basic | 63...

  • Page 66: Temperature Calibration Procedure

    Press the keys. Press the keys. Destination value e.g. 36.4 °C 4. Accept the destination value: Press the key. The temperature display momentarily shows “CAL”, Then the corrected actual value (measured destination value 36.4 °C) is displayed. CO2 Incubator Basic...

  • Page 67: Preparing The Co Calibration

    The measuring sample is withdrawn through the seal-able measurement opening of the glass door or through the access port at the rear panel of the device. The comparison measurement must be performed when the device is completely stable. CO2 Incubator Basic | 65...
  • Page 68 4. Remove measuring probe, plug measurement opening and close doors. 5. Calibrate CO control as described in Section “CO2 calibration procedure”. Measurement example: nominal value: 5%  Measured value: 5.6%  CO2 Incubator Basic...

  • Page 69: Co 2 Calibration Procedure

    The CO display momentarily shows “CAL”, then the corrected actual value (measured destination value 5.6%) is displayed. 5. Cancel the calibration process: Press any key. The temperature display and the CO display show the actual values. CO2 Incubator Basic | 67...
  • Page 70 7. Install retainer [1]. 8. Connect gas hose to particle filter sleeve and secure it using hose clamp. Check to see if the gas hose is securely seated on the sleeve. CO2 Incubator Basic...

  • Page 71: Replacing The Device Fuses

    2. To remove the fuse holder, squeeze the two locking tabs and pull holder [3] out of fuse compartment. 3. Remove faulty fuse from holder and install new fuse. 4. Slide fuse holder into fuse compartment and press holder on until locking tabs are fully engaged. CO2 Incubator Basic | 69...

  • Page 72: Replacing The Door Seal

    2. Position new seal at a corner [2] and press seal retaining rail [4] into slot. 3. Make sure that the retaining rail taper is positioned correctly in the slot [1] and that the seal is flush with the door frame. CO2 Incubator Basic...

  • Page 73: Chapter 11 Parts And Accessories

    Date Purchased  NOTE! Repairs: Use only original parts that have been tested and approved by VWR. The use of other parts presents potential hazards and will make the warranty void! Additional parts: For additional parts please contact VWR Service Organization.
  • Page 74 Chapter 11 | Parts and accessories CO2 Incubator Basic...

  • Page 75: Chapter 12 Technical Data

    5 ... 10 20 °C Temperature recovery time, at 37 °C, door open 30 seconds < 10 (to 98% of initial value) Heat transfer to environment: at 37 °C kWh/h 0.06 during 90 °C decontamination kWh/h 0.26 CO2 Incubator Basic | 73...
  • Page 76 Circuit breaker G 16 Rated input 0.64 (120 VAC) EMC class OTHERS Sound pressure level (DIN 45 635, Part 1) dB(A) < 50 Relative humidity of environment % rH max. 80 Location elevation m NN max. 2000 CO2 Incubator Basic...
  • Page 77 Technical data | Chapter 12 Average gas consumption: Figure 24 – CO consumption CO2 Incubator Basic | 75...
  • Page 78 Chapter 12 | Technical data CO2 Incubator Basic...

  • Page 79: Chapter 13 Disposal

    All device components can be discarded properly after they have been decontaminated properly. NOTE! Recycling service: VWR offer, for a small fee, an environmentally compatible recycling service for discarded devices! List of used materials: Component Material...
  • Page 80 Glass screen Soda-silicate glass Glass door seal, measurement opening Tempered silicone Sensor block Stainless steel 1.4301 Blower wheel Stainless steel 1.4305 Measuring cell baseplate seal Tempered silicone Cables Plastic-sheathed copper flexible Packaging Corrugated board, polyethylene film, and styrofoam CO2 Incubator Basic...

  • Page 81: Chapter 14 Device Log

    Device log Record following information here: Nameplate information  Work carried out  Maintenance work  Repairs  CO2 Incubator Basic | 79...
  • Page 82 Chapter 14 | Device log Device type: Part number: Serial number: Service number: Location: Operator's note: Work carried out Notes Date Signature CO2 Incubator Basic...

  • Page 83: Chapter 15 Certificate Of Decontamination

    Certificate of decontamination CO2 Incubator Basic | 81...
  • Page 84 Chapter 15 | Certificate of decontamination CO2 Incubator Basic...

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