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3-7-2 Protocol steps and
Thermo Fisher Scientific
When you create a protocol, you must define the following for each step:
options
•
Reagent: This assigns a reagent to the selected step, and displays a
color marking.
•
Duration (in minutes): This defines how long this particular
protocol step takes.
•
Temperature: You can define the temperature inside the chamber
for the given protocol step, and adjust it within preset limits. Be
especially careful adjusting the temperature for paraffin, that it does
not fall below the minimum paraffin temperature (see Section 2-5-6
Editing processing parameters). Also note that the highest reagent
temperature you can use for a chamber is 65°C.
•
Pressure/vacuum: Among the protocol steps, you have the possibility to
choose pressure or vacuum, or a change between pressure and vacuum.
You can also define:
•
Fixed End Time: This defines the duration of a wait step where
specimens remain so that the protocol will finish at the programmed
end time.
Note!
Wait Step: There can only be one wait step in any protocol. When
determining a wait step, take into consideration individual tolerability of
the sample with a longer retention period in the appropriate reagent.
Idle time (sec): Choose from the list the time when the air will
•
be pumped after emptying the chamber. By entering longer idle
periods reagent carry-over and the escape of vapors on opening of
the chamber can be reduced.
Note!
Changing colors: See Section 2-5-7 Editing reagent groups, to find out
how to change the reagent colors.
routine operations
Editing and Creating Protocols
User Guide STP420D/STP420ES
83
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