Okolab Bold Line H101-CRYO-BL Manual
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Okolab Bold Line H101-CRYO-BL Manual

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Summary of Contents for Okolab Bold Line H101-CRYO-BL

  • Page 1 H101-CRYO-BL Manual...
  • Page 2 This page was left blank...

  • Page 3: Table Of Contents

    Equipment required ......................9 Optional equipment......................9 T-Unit connection ports ..................... 10 H101-CRYO-BL. INSTALLATION AND USER GUIDE ..................10 How to connect the T-Unit to OKOLAB BOLD LINE Gas Controller........... 13 USER INTERFACE ............................. 14 Home page ........................14 Colors meaning ......................14 6.1.1...
  • Page 4 Display scheme ....................... 27 Adjustment of the pump outflows ..................29 Pump output ........................29 Standard Cooling value ...................... 30 MAINTENANCE OF THE T-UNIT ........................31 WATER BATH MAINTENANCE ......................... 31 SUPPORT ..............................33 QR Codes for Support ......................33 10.1 Support Contacts ......................

  • Page 5: Preface

    The H101-CRYO-BL can be operated via the touch screen interface OKO-TOUCH (not included), via OKOLAB DATA-LOG Software (not included) or via any Third Party Software. The H101-CRYO-BL is compatible with any Okolab Bold Line Gas Controller. In this case, a single OKO-TOUCH operates both, the Bold Line Gas Controller and T-Unit.

  • Page 6: Symbols Description

    2 SYMBOLS DESCRIPTION The following symbols identify important information to note: CAUTION or WARNING: This symbol warns you about the risk of electrical shock. CAUTION or WARNING or IMPORTANT: This symbol warns you of circumstances or practices that can affect the instrument’s functionality. Please refer to accompanying documents. Tip - Supplies you with helpful suggestions.

  • Page 7: Safety Notes

    OTES Before operating the equipment please read carefully the instructions and the safety notes. If you have any questions, please contact Okolab. The equipment must only be used as intended and as described in this Manual. Equipment should only be operated by technically qualified personnel.
  • Page 8 We reserve the right to make technical variations. IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE, LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY...

  • Page 9: System Overview

    (to be connected to the colour-related connector of the water-bath) 8. #1 24V-DC Power Adapter • Okolab incubating chamber with lid and base, chamber riser with one or more plate adapters • OKO-TOUCH, includes: RS232 Serial cable. To connect the Touch Screen to the T-Unit •...

  • Page 10: T-Unit Connection Ports

    Figure 1: T-Unit Rear Panel Overview 1. OKO-TOUCH RS232 serial port. 2. Power Input. 3. Objective Heater (optional) connector. 4. Water Bath CRYO connector. 5. USB port. 6. General Purpose Input Output (GPIO) port. 7. NTC Thermistor port. 8. Gauge Thermocouple port. H101-CRYO-BL.
  • Page 11 Figure 2. H101-CRYO-BL Standard Connection 1. Connect the Water Bath CRYO to the port labeled ‘Water Bath’ on the rear panel of the T-Unit (see Figure 2). 2. Plug the NTC Connector on the port labeled ‘Ambient Temperature’ located on the rear panel of the T-Unit (see Figure 2).
  • Page 12 Figure 3. OKO-TOUCH Connection 5. Connect the TUBE-S and TUBE-T between Okolab Chamber Incubator and the Water Bath CRYO (see Figure 4). Figure 4. Tubing connection between Okolab Chamber and the Water Bath CRYO 6. Connect the 24V-DC Power adapter to the T-Unit.

  • Page 13: How To Connect The T-Unit To Okolab Bold Line Gas Controller

    initially yellow. When the temperature set-point is within the set tolerance range, the indicator light will turn green. Tip ►Wait until system has reached the steady state before loading your samples. The T-Unit can be used as a stand-alone device or in combination with any one of the Bold Line Gas Controllers.

  • Page 14: User Interface

    SER INTERFACE Current Temperature value Temperature set-point Temperature chart Touch here to know generic info about Control Unit and running time Touch here to access system options and settings Touch here to go to Chart history lenght the OVERVIEW page set at 1 h Maximum T °C reached within the time-period Minimum T °C reached within the time-period...

  • Page 15: Changing Set Points

    The RED indicator means that there could be a problem with the unit. If after 20-30 minutes the Red light does not turn to Green, turn the system off, wait 5 minutes and turn it back on. If the problem persists contact Okolab at http://www.oko-lab.com 6.1.2 Changing Set Points To change a set point, simply touch the corresponding tab.
  • Page 16 Figure 9. Chamber Mode Setting In this configuration the water bath temperature is controlled. For maximum of accuracy, once selected the Chamber feedback mode, the operator should calibrate the difference of temperature between the water bath temperature and the specimen temperature (by sticking the fine gauge thermocouple into a reference well inside the Chamber Incubator), so to find for instance that to keep the specimen temperature at 37°C, the water bath temperature has to be at 40°C.
  • Page 17 Figure 11. Free Sensor attached on the internal base of the Petri Dish Tip ► It is recommended using an Okolab SENSOR LID (available for purchase) to secure the Fine Gauge Thermocouple in place in the Reference Well during calibration or operation in Sample Feedback Mode (see Figure 12).

  • Page 18: Objective Heater (Used Only In Exceptional Cases)

    • Once you have done close the chamber and wait for 2 minutes in order to let the environment achieve steady state; press on Incubator Closed and the temperature controller will resume operating normally. Figure 13. Open incubator Opening the incubator can lead to controller instability since both the specimen and the inside of the chamber are exposed to the external environment.

  • Page 19: Thermal Cycles

    Figure 15. Objective Heater Calibration Page The temperature of the Objective Heater is factory calibrated at room temperature of 23°C, with a 3 degrees offset relative to the sample set-point temperature of 37°C. This offset value is appropriate for a set point of 37°C.
  • Page 20 Figure 17. Thermal Cycles Subpage You can decide whether setting a new thermal cycle (“New”) or recalling a previously saved one (“Load”). To set a new thermal cycle, first choose the two temperatures between which you want to switch (SP1: Set Point 1 and SP2: Set Point 2).

  • Page 21: Status

    Figure 19. Thermal cycle Summary In the last step you can schedule the time you want the thermal cycle to start. Figure 20. Cycle Start Time At set time the cycle will start. A thermal cycling icon will be displayed on the Homepage as a reminder. Trying to change set points or starting a new thermal cycle while a different one is still running will open the window in Figure 21.

  • Page 22: External Sensors

    You may have to refer to this window when asking for Okolab technical support. Figure 22. Status page 6.2.1.5 External Sensors There are two external temperature sensors connected to the T-Unit. A white thermistor is to be placed in air to monitor the Room Temperature.

  • Page 23: Touch Screen

    Figure 24. Digital input 6.2.2 Touch Screen Touch Screen Settings are adjusted by following the two steps below (look at the touching finger) Figure 25. Touch screen settings 6.2.2.1 Options In this window you can set the scale of the X axis (time duration) of the graphs displayed in the main page and the buzzer sound frequency.

  • Page 24: Visual Effects

    Figure 27. How to enter in Alarms page 6.2.2.3 Visual Effects To enter the display visual effects menu, press on “Visual Effects” icon Figure 28. Visual effects page The Top LED settings allows you to when the Top LED should be illuminated: if you select Never, the Top LED will never illuminate, if you select On Alarm, the Top LED will illuminate only when theH401-T- CONTROLLER is in Alarm Status (see paragraph 6.1.1), if you select Always, the Top LED will always illuminate.

  • Page 25: Alarms

    To use this system feature, refer to the Data Logging quick instructions. Figure 32 shows an overview of the complete system displaying current values of all controlled parameters. Similarly to the Status Page you may have to refer to this window when asking for Okolab technical support.

  • Page 26: Overview Page

    Figure 32.Overview page The Glance mode setting defines the color of the digits displayed in Glance Mode. If you select White, the digits displayed in Glance Mode are white. If you select Dynamic the color of the digits in Glance Mode follows the color coding reported in paragraph 6.1.1 Pressing on ‘Glance Mode’...

  • Page 27: Water Bath-Cryo Appendix

    CRYO PPENDIX The thermostatic water bath included in Okolab incubation system has been tested in our laboratories. The water bath control parameters were stored to obtain the maximum performances from the system in terms of thermal stability and fast temperature recovery.
  • Page 29 Usually the Knob is positioned in configuration 2, called medium position. If water doesn’t circulate in the chamber, causing thermal uniformity absence please check the Knob position otherwise set it in position 1 The level of circulation pump is automatically set at the standard value 2. This value 2 ensures good circulation and thermal uniformity in the incubation chamber.
  • Page 30 The display shows the current bath temperature, COOL and the set operating mode. To alter the cooling setting mode, press the key on the left. The display flashes (approx 4 sec.) During this time, start to set the operating mode with one of the keys. 0 = Refrigeration system OFF 1 = Refrigeration system ON 100% capacity (Only cooled system) A = automatic operation...
  • Page 31 Standard Cooling value for the Okolab stage incubator is ‘A’ automatic refrigeration. To cool the sample at a temperature lower than the room temperature you can set COOL to 1 value. Please contact the Okolab service centre to have more details.
  • Page 32 • To protect the Water Bath fluid for a long period of use: • 1ml/l water of Aqua Resist® water bath protective media Tip ► When using temperatures in the range of 0~50°C, use a mixture of 60% ethylene glycol and 40% water in order to prevent the freezing of the medium (the solution will freeze at -45°C).
  • Page 33 10 S UPPORT In order to send directly an email to the Okolab staff, you can use the QR codes shown in the following images: • • For commercial info: For technical info: Figure 35. QR code for commercial info contact Figure 36.QR code for technical info contact...
  • Page 34 Incorrect operations are often mistaken for malfunction. If you think that there is something wrong with a component, see the troubleshooting scheme below. If the problem persists even after troubleshooting as described below, please ask for Okolab support Symptom Probable cause...
  • Page 35 12 T ECHNICAL PECIFICATIONS T-Unit Technical Specifications Temperature Range: from 10/15°C BELOW Room temperature (depending on chamber model) to 60°C Temperature Sample Temperature range: 3°C above ambient temperature to 50°C Step size: 0.1°C Accuracy:0.2°C in Sample Mode and in Cage Mode if room temperature remains within ± 1°C. Regime temperature time Depends on the control mode, about 30 min in chamber mode feedback Operating Temperature...
  • Page 36 Figure 2. H101-CRYO-BL. Standard Connection ..................11 Figure 3. OKO-TOUCH Connection ...................... 12 Figure 4. Tubing connection between Okolab Chamber and the Water Bath CRYO ........... 12 Figure 5. CO2-O2 UNIT-BL [0-10; 1-18] and T-UNIT connection (a-b) ............13 Figure 6. OKO-TOUCH Home page ...................... 14 Figure 7.
  • Page 37 Okolab S.r.l. accepts no responsibility for damage due to unsatisfactory packing. Upon examination of Okolab S.r.l., if the unit is found to be defective, it will be repaired or replaced at no charge. This warranty does not apply to defects resulting from any actions of the purchaser. Okolab S.r.l.

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