Gilson pipetman P2G Manual page 33

Pre-rinse the tips
Some liquids (e.g. protein-containing solutions and
organic solvents) can leave a film of liquid on the inside
wall of the tip ; pre-rinse the tip to minimize any errors
that may be related to this phenomenon.
Pre-rinsing consists of aspirating the first volume of liquid
and then dispensing it back into the same vessel (or to
waste). Subsequent volumes that you pipette will have
levels of accuracy and precision within specifications.
aspirate
Press the push-button to the first stop (this
corresponds to the set volume of liquid).
Hold the pipette vertically and immerse the tip in the
liquid (see immersion depth table, page 10). Release
the push-button slowly and smoothly (to top posi-
tion) to aspirate the set volume of liquid. Wait one
second (time depends on model, see table) ; then
withdraw the pipette-tip from the liquid. You may
wipe any droplets away from the outside of the tip
using a medical wipe, however if you do so take care
to avoid touching the tip's orifice.
First stop
Second stop
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