Pro Safety Sealed Chamber Assembly Maintenance - PRO Scientific Bio-Gen PRO200 Operating Manual

DO NOT REMOVE THE MOTOR COLLAR FROM THE MOTOR UNIT.
THE HOMOGENIZER SHOULD ONLY BE OPERATED WITH THE BOTTOM OF THE
CHAMBER ASSEMBLY RESTING ON THE TABLETOP, THE BOTTOM OF THE
COOLING OR HEATING VESSEL OR THE TOP OF A HEIGHT BLOCK.
7) Rotate the appropriate speed control dial until the desired speed is reached.
8) When the desired time period has elapsed, turn the "ON/OFF" switch position.
9) Remove the PRO Safety Sealed Assembly from the motor unit before attempting to open the chamber assembly.
Operation Techniques:
To use the homogenizer effectively, the following techniques are recommended:
WHEN IT IS NECESSARY TO USE VOLATILE, FLAMMABLE, TOXIC, PATHOGENIC,
RADIOACTIVE OR OTHER HAZARDOUS MATERIALS, STANDARD LABORATORY
PRECAUTIONS SHOULD BE FOLLOWED, SUCH AS VENTING, OPERATING IN A FUME
HOOD, ETC. THE HOMOGENIZER IS NOT MADE OF AN EXPLOSION-PROOF
1) Cooling - At revolutions above 5,000 rpm, relatively high temperatures are generated. Therefore, the chamber
should be cooled whenever operating at speeds above 5,000 rpm by immersing the chamber in an ice bath or
similar cooling vessel.
2) Ball Milling - At revolutions below 5,000 rpm, particles may be broken down by using hard materials, such as
glass beads. The homogenizer will act as a "ball mill" agitating the chamber while the hard materials do the
work. Since sharpness is not essential, a dull knife blade should be used.
3) Homogenizing - For more complete homogenization of some materials, it is helpful to rock the motor unit
slightly to churn up the specimen within the chamber.
2) Adding a grinding medium - To attain the desired effect (cell breakdown), a combination of procedures may be
necessary. First, homogenize the specimen in a solution using speed and a sharp knife blade. Then change the
knife blade, add a grinding medium and run the homogenizer at a maximum speed of 5,000 rpm for ten to
fifteen minutes.

PRO Safety Sealed Chamber Assembly Maintenance:

Regular maintenance and inspection/replacement of wearing generator parts is recommended and will thereby extend
the life of this valuable piece of equipment.
Please refer to the PRO Quick Connect Generator Maintenance Section for specific maintenance instructions for
maintaining the PRO Quick Connect Generator in your PRO Safety Sealed Chamber Assembly.
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CONSTRUCTION.