Operating Procedures; Dismantling The Pro Quick Connect Generator - PRO Scientific Bio-Gen PRO200 Operating Manual

Homogenizer
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DO NOT REMOVE THE MOTOR COLLAR FROM THE MOTOR UNIT.

Operating Procedures:

1) The generator may be used with either an open chamber or within a sealed chamber assembly.
2) The ideal situation is when the diameter of the generator is as large as the container will allow.
3) Insert the generator into the medium that is to be homogenized. The depth to which the generator is inserted
should be 1/3 of the liquid's height measured from the bottom of the container. According to the properties of
the material, it may not be necessary to insert the generator to this depth. In case of heavy sediments, the
generator may have to be positioned lower, but the generator must never come in contact with the bottom of
the vessel.
4) MID-BEARING NOTE: All generators that are 120mm in length and longer contain a mid-bearing, which is
located inside the generator approximately half way up the shaft. The mid-bearing must be lubricated by the
sample/liquid during processing, therefore the sample volume should cover about half the length of the
generator.
5) Start the drive unit at the lowest possible speed and then slowly increase the speed to the desired level.
6) It is advisable that the generator is positioned within the container off-center. This off-center location will help
minimize vortexing.
7) The generators have two (2) sets of venting holes. One set is located approximately 1" from the bottom of the
generator and the second set is approximately 1" below the bottom of the generator collar. The lower hole aids
in keeping the lower bearing lubricated, while the top hole allows venting of any liquid that might be forced up
into the tube. There is no need to avoid liquid from entering the lower set of holes.
8) Once you have reached the level of processing that is required, reduce the motor speed and start to slowly
remove the generator from the container while the motor is still running. It is important that this is done slowly
as you want to spin all medium from the generator back into the container and not outside of the container.
9) After spinning the medium off the generator, the motor can be turned off and the generator totally removed
from the container.

Dismantling the PRO Quick Connect Generator:

Dismantling 5mm generators
The following instructions are for the dismantling of only 5mm generators ONLY.
1. Unscrew the rotor knife from the bottom of the rotor shaft. Insert the 1/4" hex key (supplied in the tool kit) into
the end of the rotor shaft collar and insert the screwdriver (supplied in the tool kit) into the rotor knife and turn
the hex wrench counterclockwise.
2. Remove the rotor knife from the bottom of the generator tube and collar assembly.
3. Draw the rotor shaft and rotor shaft collar assembly upwards out of the tube and collar assembly. The PTFE
(Polytetrafluoroethylene) washer can be removed from the rotor shaft.
4. Remove the lower bearing from the bottom of the tube and collar assembly. The lower bearing should be
replaced when worn before the rotor knife starts to rub against the side of the stator.
5. The rotor shaft collar assembly can be removed from the rotor shaft by loosening the set screw located at the
side of the rotor shaft collar using the 5/64 hex wrench. (supplied in the tool kit).
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**CAUTION**

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This manual is also suitable for:

Pro250Hv-04751-2501-02200

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