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b.
Sterilizing by humid heat – This means sterilizing with steam at a pressure of 2 bar above and a
temperature of 120 o C.
i. PRO Generators are heat resistant up to 390° F / 198° C.
c. Sterilizing by hot air - Hot air sterilization is normally carried out at 160 to 190 o C.
i. PRO Generators are heat resistant up to 390° F / 198° C
d. Flaming - This method can be used, however, it is only effective on external surfaces.
i. PRO Generators are heat resistant up to 390° F / 198° C
PRO Sealed Chamber Assembly
Description:
With PRO Safety Sealed Chamber Assemblies, various chamber assemblies (stainless steel, glass, and plastic) are used for
the homogenization of materials in the liquid/liquid, liquid/solid, and solid/solid states. Homogenization and mixing will
normally be completed within 30 seconds to one minute. At revolutions higher than 8,000 rpm the time of mixing should
be limited to three minutes with dry mixtures and ten minutes for liquids. To homogenize solid materials it is advisable
to chop up pieces larger than 1.0 cm in diameter before putting them into the chamber. All homogenizing occurs in
safety-sealed, tubes, glass chambers or stainless steel chambers. This allows for multiple levels of protection, the design
provides a two way barrier, protecting the operator from hazardous aerosols generated during processing while it
isolates the sample from external contaminants.
Safety Sealed Assemblies with PRO Quick Connect Generator
Assembly of the Safety Sealed Assemblies with PRO Quick Connect Generator:
1)
Assemble the cover/cover plug and rotor shaft assembly. Load the material to be processed into the chamber.
2)
Make certain that the chamber is filled only to the recommended volume level for that specific chamber.
3)
Attach the filled chamber to the cover and cover plug/rotor shaft assembly.
4)
Screw the chamber cover onto the chamber. Note that the threads on the chamber and cover are left hand
threads and are screwed together by turning the cover counter clockwise. These two parts should be tightened
securely.
Attachment of the Safety Sealed Assemblies with PRO Quick Connect Generator to the
Homogenizer:
1) All PRO Safety Sealed Assemblies are fitted to the motor unit by inserting the upper end of the
generator into the collar end of the motor.
2) Align the vertical slots in the PRO Safety Sealed Assembly with the locating pins in the motor collar by
rotating the PRO Safety Sealed Assembly.
3) Once aligned, push the PRO Safety Sealed Assembly inward as far as possible and turn the PRO Safety
Sealed Assembly left. The collar is spring loaded so you will feel some resistance as you push the
generator inward and turn it.
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