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Summary of Contents for Molecular Devices EMax Plus
- Page 1 EMax® Plus Microplate Reader User Guide 5032018 C October 2018...
- Page 2 For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.
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Page 3: Table Of Contents
Contents Safety Information Warnings, Cautions, Notes, and Tips Chapter 1: Introduction Computer Integration Absorbance Read Mode Endpoint Read Type Kinetic Read Type Chapter 2: Setting Up the Instrument Connecting Instrument Cables Chapter 3: Getting Started Selecting the Instrument in the Software SoftMax Pro Protocol Settings Loading and Unloading Plates Chapter 4: Maintenance... -
Page 4: Safety Information
Follow the related safety information. The following user attention statements might be displayed in the text of Molecular Devices user documentation. Each statement implies the amount of observation or recommended procedure. - Page 5 For products under the requirement of the WEEE directive, contact your dealer or local Molecular Devices office for the procedures to facilitate the proper collection, treatment, recovery, recycling, and safe disposal of the device. - - California proposition 65 requires businesses to provide warnings to Californians about significant exposures to chemicals that cause cancer, birth defects, or other reproductive harm.
- Page 6 Electrical Safety To prevent electrical injuries and property damage, inspect all electrical equipment before use and report all electrical deficiencies. Contact Molecular Devices technical support for equipment service that requires the removal of covers or panels. To prevent electrical shock, use the supplied power cord and connect to a properly grounded wall outlet.
- Page 7 Safety Information Moving Parts Safety The plate carrier on the EMax Plus moves in and out of the read chamber during the read, and then returns to the home position after the read ends. If the plate carrier does not return to the home position, select the Operations tab and click Initialize.
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Page 8: Chapter 1: Introduction
The instrument can read 96-well plates to run protocols with the visible Absorbance (ABS) read mode with the Endpoint and Kinetic read types. The high sensitivity and flexibility of the EMax Plus Microplate Reader make it useful for applications in the fields of biochemistry, cell biology, immunology, molecular biology, and microbiology. -
Page 9: Absorbance Read Mode
EMax Plus Microplate Reader User Guide To run protocols that require advanced acquisition settings or to run Absorbance read mode Kinetic read type and Spectrum read type protocols, you must connect the instrument to a computer and use the SoftMax Pro Software to operate the instrument. -
Page 10: Endpoint Read Type
Chapter 1: Introduction OD = 2 – log (%T) The factor of two comes from the fact that %T is expressed as a percent of the transmitted light and log (100) = 2. When in %Transmittance analysis mode, the instrument converts the raw OD values reported by the instrument to %Transmittance using the above formula. All subsequent calculations are done on the converted numbers. -
Page 11: Chapter 2: Setting Up The Instrument
The package contains the instrument and accessories to set up the instrument: SoftMax Pro Software, product key, and installation guide EMax Plus Microplate Reader Installation Guide USB computer connection cable AC power adapter For a complete list of the package contents, see the enclosed packing list. -
Page 12: Connecting Instrument Cables
EMax Plus Microplate Reader User Guide 6. Follow the orange string attached to the red transport lock to a screw located underneath the instrument. Carefully move or tilt the instrument away from the table to access the screw. 7. Use your fingers to remove the transport lock screw. -
Page 13: Chapter 3: Getting Started
Chapter 3: Getting Started Now that you installed the SoftMax Pro Software on the computer, removed the transport locks, and connected the cables, it is time to get started. Before you operate the instrument or perform maintenance operations, make sure that you are familiar with the Safety Information on page 1. -
Page 14: Softmax Pro Protocol Settings
EMax Plus Microplate Reader User Guide To select the instrument: 1. Select the Home tab and click the Instrument icon to display the Instrument Connection dialog. 2. From the Available Instruments list, select the communication port to which you connect the instrument. -
Page 15: Loading And Unloading Plates
Chapter 3: Getting Started 10. Save the settings as a data file or a protocol file. See the SoftMax Pro Data Acquisition and Analysis Software User Guide or the application help. Data files contain the experiment settings, the raw data the instrument collects, and the data analysis you define for the experiment. - Page 16 EMax Plus Microplate Reader User Guide 3. Select the Home tab and click . The instrument calibrates the lamp and then moves the plate carrier into the read chamber to start the read. 4. After the read ends, the plate carrier returns to the home position. If the plate carrier is not in the home position, select the Operations tab and click Initialize.
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Page 17: Chapter 4: Maintenance
Chapter 4: Maintenance Perform only the maintenance tasks described in this guide. Contact a Molecular Devices service engineer to inspect and perform a preventive maintenance service on the instrument each year. See Obtaining Support on page Before you operate the instrument or do maintenance operations, make sure you are familiar with the safety information in this guide. -
Page 18: Replacing The Halogen Lamp
EMax Plus Microplate Reader User Guide CAUTION! Do not use abrasive cleaners. Do not spray cleaner directly onto the instrument or into any openings. Do not let water or other fluids drip inside the instrument. Always turn the power switch off and disconnect the power cord from the main power source before using liquids to clean the instrument. - Page 19 Chapter 4: Maintenance 6. Carefully set the cover plate on the workbench. Some wires remain attached to the cover plate. WARNING! WARNING! The halogen lamp can become hot when in use. Before touching the area near the lamp, make sure that the lamp has had time to properly cool to a safe temperature.
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Page 20: Adjusting The Lamp
19. Start the SoftMax Pro Software and do a lamp adjustment. Note: After you replace the halogen lamp, if the new lamp does not flash during a read or the lamp adjustment fails, contact Molecular Devices technical support. See Obtaining Support on page... -
Page 21: Obtaining Support
WARNING! BIOHAZARD. It is your responsibility to decontaminate components of the instrument before you return parts to Molecular Devices for repair. Molecular Devices does not accept items that have not been decontaminated where it is applicable to do so. If parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated. -
Page 22: Appendix A: Instrument Specifications
Appendix A: Instrument Specifications Absorbance Measurement Specifications Item Description Wavelength range 400 nm to 750 nm Wavelength selection Filters Installed filters 405 nm, 450 nm, 492 nm, 562 nm, 570 nm, 595 nm, 620 nm, 650 nm Photometric range 0.000 to 3.300 OD Photometric resolution 0.001 OD Photometric accuracy, 0.5% 1.0 OD at 450 nm Photometric precision 0.25% (repeatability), 1.0 OD at... -
Page 23: Physical Specifications
EMax Plus Microplate Reader User Guide Physical Specifications Physical Specifications Item Description Environment Indoor use only Power requirements, 12 VDC, 4 amps (50 VA) main unit Power requirements, 100 VAC to 240 VAC ±10%, 1.5 amps, frequency range: 50/60 Hz power adapter Dimensions 31.50 cm W x 18.20 cm H x 43.50 cm D (12.40 in. -
Page 24: System Dimensions
Appendix A: Instrument Specifications System Dimensions In the following drawings, the dimensions are show in centimeters and inches. Front View Dimensions Item Description 1 Height: 18.20 cm (7.17 in.) 2 Width: 31.50 cm (12.40 in.) Side View Dimensions Item Description 1 Height: 18.20 cm (7.17 in.) 2 ... - Page 25 The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ©2018 Molecular Devices, LLC. Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents All rights reserved. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES...
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