Softmax Pro Protocol Settings - Molecular Devices EMax Plus User Manual

EMax Plus Microplate Reader User Guide
To select the instrument:
1. Select the Home tab and click the Instrument icon to display the Instrument Connection
dialog.
2. From the Available Instruments list, select the communication port to which you
connect the instrument.
If the communication port does not appear in the list, click Refresh.
3. Click OK.

SoftMax Pro Protocol Settings

The SoftMax Pro Software Settings dialog enables you to define the instrument settings to
read the plate.
1. Turn on the instrument and start the SoftMax Pro Software. See
13.
2. Select an instrument. See
3. In the Navigation tree, select a Plate section.
4. On the Home tab, click Acquisition Settings
5. Select a read type.
6. Select the Wavelengths category:
a. Click the Number of Wavelengths drop-down and select to use up to six
b. Click the drop-down for each wavelength and select the wavelengths from the list of
7. Select the Read Area category:
8. For the Kinetic read type, select the Timing category:
OR
9. Click OK.
14
Selecting the Instrument in the Software on page
wavelengths.
installed filters.
To read all the wells in the plate, select the Select All check box.
To select a contiguous, rectangular region on the plate, drag the cursor to select the
wells to be read. Columns do not need to start with "1" but must be contiguous. If
you select a subset of wells, the instrument reads the entire plate, but reports the
data for the wells you select only.
In the Total Read Time field, enter the amount of time for the total run.
In the Interval field, enter the amount of time to wait between reads.
The software calculated the minimum interval when calibrating based on the total
run time and the interval you enter and displays the interval before the first read,
before each read, and the number of reads. The calculation depends on many
factors including the number of wavelengths, the number of wells to read, and the
distance the instrument filter wheel must move.
Click Single Point Read to set the Total Run Time and the Interval to the same value.
Use this to only read one time point.
Getting Started on page
to display the Settings dialog.
13.
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