Endpoint Read Type; Kinetic Read Type - Molecular Devices EMax Plus User Manual

OD = 2 – log
The factor of two comes from the fact that %T is expressed as a percent of the transmitted
light and log
When in %Transmittance analysis mode, the instrument converts the raw OD values
reported by the instrument to %Transmittance using the above formula. All subsequent
calculations are done on the converted numbers.
Applications of Absorbance
Absorbance-based detection is commonly used to evaluate changes in color or turbidity,
permitting widespread use including ELISAs, protein quantitation, endotoxin assays, and
cytotoxicity assays.

Endpoint Read Type

For the Endpoint read type, a read of each plate well is taken in the center of each well, at a
single wavelength or at multiple wavelengths.

Kinetic Read Type

For the Kinetic read type, the instrument collects data over time with reads taken in the
center of each well at regular intervals.
The values calculated based on raw kinetic data include VMax, VMax per Sec, Time to VMax,
Onset Time, and more. Kinetic reads can be single-wavelength or multiple-wavelength reads.
Kinetic analysis can collect data points in time intervals of seconds, minutes, hours, or days.
Kinetic analysis has many advantages when determining the relative activity of an enzyme in
different types of plate assays, including ELISAs and the purification and characterization of
enzymes and enzyme conjugates. Kinetic analysis can provide improved dynamic range,
precision, and sensitivity relative to endpoint analysis.
5032018 C
(%T)
10
(100) = 2.
10
Chapter 1: Introduction
10