BD FACSFlow Supply System User Manual page 21

If you observe a swollen waste cubitainer, release of air from
the cubitainer, or a buildup of pressure, immediately replace
the air filter on the top of the waste cap assembly.
Caution! If the air filter on the top of the waste
cubitainer cap assembly becomes clogged, air cannot
be vented from the cubitainer, causing it to swell
under pressure. If you observe swelling of the waste
cubitainer, loosen the cap to relieve the pressure, and
immediately replace the air filter. Removing the air
filter or the waste cubitainer cap assembly without
first relieving the pressure might generate an
unnoticeable aerosol. Use appropriate precaution
when troubleshooting a clogged air filter. Wear
suitable protective clothing, eyewear, and gloves.
4. Retighten the cap assembly on the waste cubitainer.
5. Toggle the power switch on the right side of the BD FACSFlow
supply system control panel to the On position.
The System On indicator and the Power indicator in the toggle
switch illuminate.
6. Check the sheath fluid supply line and the sheath filter for the
presence of air.
If you see air in the line or the filter, perform steps 7 and 8. If
not, go to step 9.
7. Verify that the sheath plenum is depressurized.
8. If there is air in the sheath fluid supply line, locate the Prime
button under the Pump Fill indicator light, and then press and
hold down the Prime button for one-half second.
Use only the Prime button if there is air in the sheath fluid
supply line. If the Prime button does not activate the pump
cycle, the BD FACSFlow supply system will clear the air in the
sheath fluid supply line during the next pump cycle.
9. Turn on the cytometer.
10. Allow 20 to 30 seconds for the sheath plenum to pressurize.
Chapter 3: Operation 21