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BD FACSFlow Supply System User Manual

Supply system for bd lsrfortessa cytometers
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For Research Use Only
23-11954-01
10/2020
Becton, Dickinson and Company
BD Biosciences
2350 Qume Drive
San Jose, CA 95131 USA
.
bdbiosciences
com
ResearchApplications@bd.com
BD FACSFlow™
Supply System
For BD LSRFortessa™ Cytometers
BD Biosciences
European Customer Support
Tel +32.53.720.600
help.biosciences@bd.com
User's Guide

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Summary of Contents for BD FACSFlow Supply System

  • Page 1 BD FACSFlow™ Supply System User’s Guide For BD LSRFortessa™ Cytometers For Research Use Only 23-11954-01 10/2020 Becton, Dickinson and Company BD Biosciences European Customer Support BD Biosciences Tel +32.53.720.600 2350 Qume Drive help.biosciences@bd.com San Jose, CA 95131 USA bdbiosciences ResearchApplications@bd.com...
  • Page 2 BD Biosciences. The information in this guide is subject to change without notice. BD Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments. Although this guide has been prepared with every precaution to ensure accuracy, BD Biosciences assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information.

  • Page 3: Table Of Contents

    Replacing the waste cubitainer ........27 Flushing the BD LSRFortessa system ....... . . 30 Flushing the BD LSRFortessa system with a BD HTS .
  • Page 4 BD FACSFlow Supply System User’s Guide Chapter 5: Troubleshooting Troubleshooting ..........44...

  • Page 5: Chapter 1: About This Guide

    About this guide This guide describes the setup and operation of the BD FACSFlow supply system. The BD FACSFlow Supply System User’s Guide is intended for laboratory personnel and flow cytometry operators. Application-specific instructions are not included in this guide.

  • Page 6: Conventions

    BD FACSFlow Supply System User’s Guide Conventions The following table lists the safety symbols used in this guide to Introduction alert you to potential hazards. Safety symbols Symbol Meaning Caution alert Identifies a hazard or unsafe practice that could result in...

  • Page 7: Technical Support

    Troubleshooting (page 43) If additional assistance is required, contact your local Contacting BD Biosciences technical support representative or supplier. technical support When contacting BD Biosciences, have the following information available: • Product name, part number, and serial number • Any error messages •...
  • Page 8 This page intentionally left blank...

  • Page 9: Chapter 2: System Overview

    System overview This section includes these topics: • Product description (page 10) • Principles of operation (page 12) • Control panel (page 13) • Sheath plenum enclosure (page 16) • Specifications and requirements (page 17)

  • Page 10: Product Description

    BD LSRFortessa™ flow cytometers. This topic describes the system and its components. Product The BD FACSFlow supply system is For Research Use Only and is not for use in diagnostic or therapeutic procedures. The system has classification not been validated for clinical use.
  • Page 11 Chapter 2: System overview Item Description Power switch Cabinet base Air filter Waste cubitainer probe Probe holder Sheath cubitainer probe Plenum Caution! Do not move the BD FACSFlow supply system by pulling on the probe holders because they might break off.

  • Page 12: Principles Of Operation

    When the probe in the sheath plenum detects a specified low level, it signals the BD FACSFlow supply system to pump sheath fluid from the 20-L cubitainer to the sheath plenum. Sheath and waste...

  • Page 13: Control Panel

    This topic describes the location and function of the controls and indicators on the control panel. You can access and monitor all of the BD FACSFlow supply system functions from the control panel. Control buttons The following figure and table show the location of the control...
  • Page 14 BD FACSFlow Supply System User’s Guide Item Button Function Alarm Shuts off the audible alarm. The indicator light remains flashing until the situation is remedied. Prime Pumps sheath fluid to the sheath plenum until it reaches the default operational level.
  • Page 15 Indicates the power-on test of the internal electronics has failed. Power off the system, wait for 15 seconds, and turn the system on again. The System On indicator light should now be steady. If the indicator is still flashing, contact your BD Biosciences service representative.

  • Page 16: Sheath Plenum Enclosure

    BD FACSFlow Supply System User’s Guide Sheath plenum enclosure Introduction This topic describes the sheath plenum enclosure. Description The sheath plenum enclosure holds the sheath plenum and the sheath filter. Caution! The sheath plenum and the sheath filter must be replaced annually.

  • Page 17: Specifications And Requirements

    Width: 62 cm (24.4 in.) • Depth: 73 cm (28.7 in.) • Weight: 22 kg (48.4 lb). The weight of the unit does not include the weight of full BD FACSFlow sheath or waste cubitainers. • Input voltage/Hz: 100–240, VAC 50–60 Hz Electrical •...
  • Page 18 BD FACSFlow Supply System User’s Guide • Temperature: 16°C–29°C Operating environment • Humidity: 10–90% relative humidity at 35°C, non-condensing • Air filtering: no excessive dust and smoke...

  • Page 19: Chapter 3: Operation

    Operation This section includes these topics: • Starting up the system (page 20) • Shutting down the system (page 22) • Responding to alarms (page 22)

  • Page 20: Starting Up The System

    It is important to perform the following steps in the order given. Procedure This will allow the pump in the BD FACSFlow supply system to fill the sheath plenum without creating back pressure. To start up the system: 1.
  • Page 21 4. Retighten the cap assembly on the waste cubitainer. 5. Toggle the power switch on the right side of the BD FACSFlow supply system control panel to the On position. The System On indicator and the Power indicator in the toggle switch illuminate.

  • Page 22: Shutting Down The System

    Procedure To shut down the system: 1. Perform the standard cytometer shutdown procedure. 2. Shut off the BD FACSFlow supply system by toggling the power switch to the Off position, after you have turned off the cytometer. More information •...
  • Page 23 Chapter 3: Operation Procedure: Waste When the sensor probe detects that the waste cubitainer is full, the sensor triggers the Waste Full alarm. Occasionally, if fluid Full alarm accumulates around the sensor probe, the sensor can trigger the Waste Full alarm even when the waste cubitainer is not full. Caution: Biohazard! All biological specimens and materials coming in contact with them are considered biohazards.
  • Page 24 BD FACSFlow Supply System User’s Guide 8. Press the Restart button on the control panel. The indicator light switches off. Pressing Restart triggers a check of the waste sensor in the cubitainer to verify that the waste has been emptied. If the waste was emptied, the indicator light switches off.

  • Page 25: Chapter 4: Maintenance

    Replacing the waste cubitainer (page 27) • Flushing the BD LSRFortessa system (page 30) • Flushing the BD LSRFortessa system with a BD HTS (page 33) • Replacing the air filter (page 37) • Changing the sheath filter (page 39) •...

  • Page 26: Replacing The Sheath Cubitainer

    45-degree angle. 2. Place the sensor probe into the black probe holder (see the following image) on the side of the BD FACSFlow supply system unit. 3. Load the new sheath fluid cubitainer into the left side of the...

  • Page 27: Replacing The Waste Cubitainer

    Introduction solution and for replacing the waste cubitainer. You must replace the waste cubitainer whenever it gets close to being full. You can use BD FACSClean™ at full strength as a replacement for 10% bleach. Preparing 10% Prepare a 10% bleach solution using a high quality, particulate- bleach solution free bleach.
  • Page 28 BD FACSFlow Supply System User’s Guide Replacing the Caution: Biohazard! All biological specimens and waste cubitainer materials coming in contact with them are considered biohazards. Handle as if capable of transmitting infection and dispose of with proper precautions in accordance with federal, state, and local regulations.
  • Page 29 Chapter 4: Maintenance 5. Remove the neck support collar and save it for the next cubitainer. Item Description Neck support color Waste cubitainer Sheath cubitainer 6. Place the 2-inch long waste sensor probe into the 500-mL beaker containing the 10% bleach solution you have prepared. Caution! Avoid splashing the 10% bleach solution inside the rim of the cap assembly.

  • Page 30: Flushing The Bd Lsrfortessa System

    The cytometer system is shipped with a stainless steel container for About the stainless steel sheath sheath fluid. This container is replaced by the sheath cubitainer containers when the BD FACSFlow supply system is installed. Save the stainless steel container and tubing and use it when flushing the system.
  • Page 31 Chapter 4: Maintenance Fluid and air line The following diagram shows the fluid and air line connections for the system flush procedure. diagram Cytometer connector panel Sheath filter Air line Air Out Fluid In Fluid line Sheath plenum Stainless steel container Preparing to flush To prepare to flush the system:...
  • Page 32 When the STNDBY button lights (amber), press the PRIME button again. 6. Install a tube with 3 mL of 10% bleach or full-strength BD BD FACSClean solution on the SIP and put the tube support arm underneath the tube.

  • Page 33: Flushing The Bd Lsrfortessa System With A Bd Hts

    1.5% dilution of BD Detergent Solution Concentrate. Note: The BD Detergent Solution Concentrate must be diluted before use. Mix one full 15 mL bottle of BD Detergent Solution Concentrate into 985 mL of DI water to make 1 L total.
  • Page 34 The cytometer system is shipped with a stainless steel container for Using the stainless steel sheath sheath fluid. This container is replaced by the sheath cubitainer container when the BD FACSFlow supply system is installed. Save the stainless steel container and tubing and use it for the system flush procedure.
  • Page 35 Chapter 4: Maintenance The following diagram shows the fluid and air line connections for Fluid and air line diagram the system flush procedure on a system with an HTS installed. Cytometer connector panel Sheath filter Air line Air Out Fluid line Fluid In Fluid and air line connections...

  • Page 36: Replacing The Air Filter

    BD FACSFlow Supply System User’s Guide Flushing the system To flush the system: 1. Fill the stainless steel container with 2 L of 10% bleach solution (or full-strength BD FACSClean solution). Caution! Confirm that the sheath filter is not in the fluid line.
  • Page 37 Chapter 4: Maintenance 14. Reconnect the air and fluid lines coming from the sheath plenum. • Flushing the BD LSRFortessa system (page 30) More information Replacing the air filter Introduction This topic describes the procedure for replacing the air filters. An air filter is located on the cap assemblies of the sheath and waste cubitainers.

  • Page 38: Changing The Sheath Filter

    BD FACSFlow Supply System User’s Guide Item Description Waste sensor probe Air filter Silicone tubing Cap assembly Sheath sensor probe Air filter Cap assembly 2. Insert a new air filter into the silicone tubing. 3. Verify that the cap assembly on each cubitainer is tightened.
  • Page 39 Chapter 4: Maintenance More information • Replacing the sheath cubitainer (page 26) • Replacing the waste cubitainer (page 27) • Specifications and requirements (page 17) • Troubleshooting (page 44) Changing the sheath filter Introduction This topic describes how to change the sheath filter. The sheath filter is connected in-line with the sheath line.
  • Page 40 BD FACSFlow Supply System User’s Guide Sheath filter components Item Description Filter base Filter Quick-disconnect Fluid line Vent line Vent cap Removing the old To remove the old filter: filter 1. Place the cytometer in standby mode. 2. Remove the sheath filter assembly by pressing the quick-disconnect on both sides of the filter assembly, and pulling it out of the holder on the sheath plenum enclosure.
  • Page 41 Chapter 4: Maintenance Attaching the new To attach the new filter: filter 1. Connect the vent line to the new filter assembly. Twist to attach. 2. Wrap Teflon® tape around the filter threads, then connect the filter to the filter base. 3.

  • Page 42: Sensors And Tubing Locations

    BD FACSFlow supply system. Locations Caution: Biohazard! Do not disconnect the tubing from the back of the BD FACSFlow supply system or loosen the caps from the plenum or cubitainers unless the BD FACSFlow supply system is shut off. Doing so while the system is in operation can cause potentially biohazardous waste to flow out of the tubing.
  • Page 43 Chapter 4: Maintenance The following figure shows the connector positions on the back of the BD FACSFlow supply system. The table shows the functions of each sensor cable and tubing. Location Color Function black AC plug black 1 A T fuse...
  • Page 44 BD FACSFlow Supply System User’s Guide...
  • Page 45 Troubleshooting This section includes this topic: • Troubleshooting (page 44)
  • Page 46 BD FACSFlow Supply System User’s Guide Troubleshooting Introduction The tips in this topic are designed to help you troubleshoot your system. If additional assistance is required, contact your local BD Biosciences technical support representative. For up-to-date contact information, see our website, bdbiosciences.com.

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