BD FACSCanto II Manuals

Manuals and User Guides for BD FACSCanto II. We have 4 BD FACSCanto II manuals available for free PDF download: Instructions For Use Manual, User Manual, Reference Manual, Quick Reference Manual

BD FACSCanto II Instructions For Use Manual (250 pages)

Brand: BD | Category: Measuring Instruments | Size: 3 MB

Table of Contents
5
About These Instructions

11
- Conventions
  12
Chapter 1: Introduction

13
- System Components and Theory of Operation
  14
  - Flow Cytometer Components
    15
  - Fluidics Cart
    22
  - BD FACS Loader (Optional)
    25
  - Barcode Reader (Optional)
    27
- System Requirements
  28
Chapter 2: Software Windows and Toolbars

31
- BD Facscanto Clinical Software Workspace
  32
  - BD Facscanto Toolbars
    33
- BD Facsdiva Software Workspace
  34
Chapter 3: Barcode Reader Option

39
Chapter 4: Starting up

45
Chapter 5: Cytometer QC and Setup

49
Chapter 6: Running Samples with BD Facscanto Clinical Software

89
- Running an Acquisition Worklist
  90
  - Entering Information into a Worklist
    91
  - Running a Process Control
    92
  - Acquiring Samples
    93
  - Options During Acquisition
    99
  - Stopping Recording
    106
  - Importing a Worklist from SPA Software
    107
- Reviewing an Analysis Worklist
  108
  - Creating a New Analysis Worklist
    108
- Reviewing a Worklist
  111
- Logging out
  113
Chapter 7: Running Samples with BD Facsdiva Software

115
- Setting up the Global Worksheet
  116
- Recording Data
  118
  - Importing a Worklist from SPA Software
    120
- Analyzing Data
  122
  - Reusing the Analysis
    125
  - Saving the Analysis
    125
- Logging out
  127
Chapter 8: Using the Loader with BD Facsdiva Software

129
- Getting Ready
  130
- Assigning Carousels and Verifying Run Settings
  132
- Preparing the Loader
  136
- Running Samples
  139
  - Skipping or Re-Running Samples
    141
  - Adding Tubes to an Existing Carousel
    144
  - Re-Running a Carousel
    144
  - Stopping a Run
    144
  - Running a Single Tube
    145
- Running Cleaning Tubes on the Loader
  146
Chapter 9: Shutting down

147
- BD Facscanto Clinical Software
  147
- BD Facsdiva Software
  149
Chapter 10: Maintenance

153
- Scheduled Maintenance
  154
  - Emptying the Waste Container
    156
  - Purging the Fluidics Filters
    158
  - Decontaminating the Fluidics System (Long Clean)
    160
  - Replacing the Air Filter
    161
  - Replacing Fluidics Filters
    162
- Unscheduled Maintenance
  164
  - Changing a Cubitainer
    166
  - Cleaning External Surfaces
    171
  - Removing Bubbles from the Flow Cell
    172
  - Cleaning the Flow Cell
    173
  - Purging the Bubble Filter
    174
  - Decontaminating the Fluidics System for Storage
    175
  - Replacing the Bal Seal
    175
  - Reconnecting the Fluidics Cart Tubing
    181
  - Replacing the Fluidics Level Sensors
    183
  - Replacing the Fluidics Cart Fuses
    186
Chapter 11: Troubleshooting

189
Appendix A: Supplies and Replacement Parts

227
- Cytometer Supplies
  228
  - Installation Kit
    228
  - Other Replacement Parts
    229
  - Barcode Reader Parts
    229
- Consumables
  230
  - Cytometer Setup
    230
  - Reagents
    230
  - Labware
    231
Appendix B: Technical Specifications

233
- Cytometer Specifications
  234
  - Environment
    235
  - Performance
    235
  - Optics
    235
  - Fluidics
    237
  - Signal Processing
    237
- Fluidics Cart Specifications
  238
  - Capacity
    238
- BD FACS Loader Specifications
  239
Index

241

BD FACSCanto II User Manual (184 pages)

High Throughput Sampler

Brand: BD | Category: Laboratory Equipment | Size: 6 MB

Table of Contents
5
About this Guide
9
- Conventions
  10
- Technical Assistance
  11
Chapter 1: Introduction

13
- HTS Hardware Overview
  14
- Components
  16
- Cytometer Connections
  19
  - Cytometer Interface Panel
    19
  - Sample Coupler
    21
- Sample Processing
  23
Chapter 2: BD Facsdiva Software Overview

27
- Workspace Components
  28
- Plate Window
  32
  - Setup View Components
    33
  - Setup View Functions
    43
  - Analysis View Components
    48
  - Analysis View Functions
    51
Chapter 3: Running Samples

55
- Starting up
  56
  - Setting up for Plate-Based Acquisition
    59
- Creating Experiments
  64
  - Creating a Folder and an Experiment
    64
  - Setting up the Plate
    67
  - Setting up the Worksheet
    74
  - Exporting a Plate as a Template
    76
  - Assigning Keywords
    77
- Preparing for Acquisition
  78
  - Optimizing Cytometer and HTS Settings
    78
  - Performing Compensation
    81
- Acquiring Data
  82
- Analyzing Data
  86
  - Displaying Keywords
    86
  - Performing Data Analysis
    90
  - Maintaining Data
    93
- Returning to Tube-Based Acquisition
  94
  - Returning to Tube-Based Acquisition on the BD LSR II
    94
  - Returning to Tube-Based Acquisition on the BD Facscanto
    97
- Shutting down
  100
  - Quitting the Software
    101
Chapter 4: Maintenance

103
- Daily Maintenance
  104
  - Daily Cleaning
    104
  - Cytometer Inspection and Servicing
    109
- Monthly Maintenance
  111
  - Monthly Cleaning
    111
  - Surface Inspection and Cleaning
    114
- Periodic Maintenance
  114
- Unscheduled Maintenance
  136
  - Homing the Sample Probe
    136
  - Priming the HTS
    138
  - Performing a Motion Test
    138
  - Verifying the Sample Probe Position
    140
  - Reinitializing the HTS
    142
  - Inspecting Thumbscrew Fittings
    142
  - Inspecting Hexagonal Fittings
    143
  - Declogging the SIT
    143
Chapter 5: Troubleshooting

145
- HTS Troubleshooting
  146
- Acquisition Troubleshooting
  147
- BD Facsdiva Troubleshooting
  149
Appendix A: Consumables and Replacement Parts

151
- Cytometer Supplies
  152
- Maintenance Log
  154
Appendix B: Depot Repair Procedures

157
- Depot Repair Overview
  158
- Removing the HTS Unit
  159
- Cleaning the HTS Unit
  162
  - Decontaminating the Fluidics Manually
    163
  - Decontaminating External Surfaces
    167
- Unpacking the Replacement Unit
  169
  - Lifting Heavy Objects
    169
  - Unpacking the HTS Unit
    170
- Installing the HTS Unit
  170
- Packing the Unit for Shipping
  175
  - Placing the HTS into Its Shipping Container
    175
- Index
  177

BD FACSCanto II Reference Manual (158 pages)

Flow Cytometer

Brand: BD | Category: Medical Equipment | Size: 2 MB

Table of Contents
5
About this Manual

9
- Conventions
  10
- Technical Assistance
  11
Chapter 1: Introduction

13
- Intended Use
  14
- System Components
  15
  - Flow Cytometer
    16
  - Fluidics Cart
    26
  - Computer Workstation
    29
  - BD FACS Loader (Optional)
    29
  - Barcode Reader (Optional)
    30
- System Requirements
  31
Chapter 2: BD FACS Loader Option

33
- Loader Components
  34
- Using the Loader
  36
- Maintaining the Loader
  42
  - Spill Containment Area
    42
  - Cleaning External Surfaces
    43
Chapter 3: Barcode Reader Option

45
Chapter 4: Maintenance

51
- Scheduled Maintenance
  52
- Unscheduled Maintenance
  66
Chapter 5: Troubleshooting

95
- Instrument Troubleshooting
  96
- Fluidics Cart Troubleshooting
  101
- Loader Troubleshooting
  102
Appendix A: Technical Overview

105
- Flow Cytometry
  106
- Fluidics System
  106
- Optics System
  109
  - Light Scatter
    110
  - Fluorescence
    111
  - Optical Filters
    112
  - Detectors
    114
  - Detector Arrays
    114
  - Spillover
    117
- Electronics System
  119
  - Pulses
    120
  - Pulse Measurements
    121
Appendix B: Optics Configurations

125
- 4-2 Optics Configuration
  126
- 4-2-2 Optics Configuration
  130
- 5-3 Optics Configuration
  134
Appendix C: Supplies and Replacement Parts

139
- Instrument Supplies
  140
  - Installation Kit
    140
  - Other Replacement Parts
    141
  - Bar Code Reader Parts
    141
- Consumables
  142
  - Instrument Setup
    142
  - Reagents
    142
  - Labware
    143
Appendix D: Technical Specifications

145
- Cytometer Specifications
  146
  - Environment
    147
  - Performance
    147
  - Optics
    147
  - Fluidics
    149
  - Signal Processing
    149
- Fluidics Cart Specifications
  150
  - Capacity
    150
- BD FACS Loader Specifications
  151
Index

153

BD FACSCanto II Quick Reference Manual (7 pages)

Flow Cytometer

Brand: BD | Category: Measuring Instruments | Size: 0 MB

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BD FACSCanto II Manuals

BD FACSCanto II Instructions For Use Manual (250 pages)

Table of Contents

About These Instructions

Chapter 1: Introduction

Chapter 2: Software Windows and Toolbars

Chapter 3: Barcode Reader Option

Chapter 4: Starting up

Chapter 5: Cytometer QC and Setup

Chapter 6: Running Samples with BD Facscanto Clinical Software

Chapter 7: Running Samples with BD Facsdiva Software

Chapter 8: Using the Loader with BD Facsdiva Software

Chapter 9: Shutting down

Chapter 10: Maintenance

Chapter 11: Troubleshooting

Appendix A: Supplies and Replacement Parts

Appendix B: Technical Specifications

Index

BD FACSCanto II User Manual (184 pages)

Table of Contents

Chapter 1: Introduction

Chapter 2: BD Facsdiva Software Overview

Chapter 3: Running Samples

Chapter 4: Maintenance

Chapter 5: Troubleshooting

Appendix A: Consumables and Replacement Parts

Appendix B: Depot Repair Procedures

BD FACSCanto II Reference Manual (158 pages)

Table of Contents

About this Manual

Chapter 1: Introduction

Chapter 2: BD FACS Loader Option

Chapter 3: Barcode Reader Option

Chapter 4: Maintenance

Chapter 5: Troubleshooting

Appendix A: Technical Overview

Appendix B: Optics Configurations

Appendix C: Supplies and Replacement Parts

Appendix D: Technical Specifications

Index

BD FACSCanto II Quick Reference Manual (7 pages)

Table of Contents

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