Starting Up The System - BD FACSFlow Supply System User Manual

20 BD FACSFlow Supply System User's Guide

Starting up the system

Introduction
Startup guidelines
Procedure
This topic describes the procedure for starting up the system.
Follow these guidelines each time you start the system.
• Tighten all caps before operating the BD FACSFlow supply
system to avoid overflow of any containers.
• Do not operate the cytometer if the BD FACSFlow supply
system is shut off. If the BD FACSFlow supply system is shut
off while the cytometer is in operation, the waste cubitainer
can overflow and the sheath plenum can run dry.
It is important to perform the following steps in the order given.
This will allow the pump in the BD FACSFlow supply system to fill
the sheath plenum without creating back pressure.
To start up the system:
1. Check the sheath cubitainer to verify that it contains sufficient
sheath fluid for the day.
Caution! The BD FACSFlow supply system was not
designed to fill an empty sheath plenum. If you
empty the sheath plenum, manually fill it with
1,500 mL of sheath fluid before you use the
BD FACSFlow supply system.
2. Check the waste cubitainer to verify that it is not full or
swollen.
3. Loosen the cap assembly on the waste cubitainer slightly and
slowly (to a maximum of one half turn) to check for excess
pressure.