BD FACSAria Manuals

BD FACSAria Manuals

Manuals and User Guides for BD FACSAria. We have 3 BD FACSAria manuals available for free PDF download: User Manual, Startup, Qc & Shutdown Procedure, Quick Manual

BD FACSAria User Manual

BD FACSAria User Manual (276 pages)

Brand: BD | Category: Measuring Instruments | Size: 7 MB

Table of Contents

Table of Contents
3
About this Guide

9
- Conventions
  10
- Technical Assistance
  11
Safety and Limitations

13
- Laser Safety
  13
  - Laser Product Classification
    14
  - Precautions for Safe Operation
    14
- Electrical Safety
  15
- Biological Safety
  16
- General Safety
  17
- Precaution Labels
  18
- Limitations
  20
Chapter 1: BD Facsaria Cytometer Components

21
- Fluidics Cart
  22
  - Containers and Connectors
    23
  - Power and Operation
    25
- Flow Cytometer
  26
  - Fluidics Components
    27
  - Optics System
    36
  - Cytometer Electronics
    42
- Workstation
  44
Chapter 2: Theory of Operation

45
- Fluid Movement
  46
  - Sheath Flow
    46
  - Sample Flow
    48
- Signal Generation
  50
  - Light Scatter
    50
  - Fluorescent Signals
    51
- Signal Detection
  52
  - Detector Arrays
    52
  - Filters
    53
  - Detectors
    58
- Electronic Processing
  59
  - Pulse Parameters
    60
  - Laser Delay
    61
- Sorting
  62
  - Drop Formation
    63
  - Side Stream Formation
    67
  - Drop Charging
    70
  - Conflict Resolution During Sorting
    71
Chapter 3: Using BD Facsdiva Software

79
- Workspace Components
  80
- Cytometer Controls
  81
  - Fluidics Controls
    81
  - Fluidics Level Indicators
    84
  - Cytometer Configuration
    86
  - Cytometer Status Report
    89
  - Acquisition Controls
    91
- Sorting Controls
  92
  - Sort Menu
    93
  - Sort Setup
    94
  - Sort Layout
    96
  - Sort Report
    103
- Templates
  105
Chapter 4: Running Samples

107
- Cytometer Startup
  108
- Checking Performance with Cytometer Setup and Tracking
  118
  - Overview of Cytometer Setup and Tracking
    118
  - Cytometer Quality Control Using BD Facsdiva Software
    118
- Cytometer Quality Control Using BD Facsdiva Software
  119
  - Setting up Cytometer Configuration
    120
  - Preparing QC Particles
    122
  - Adjusting Area Scaling and Laser Delay
    122
  - Reusing the QC Experiment
    133
  - Tracking QC Results
    134
  - Data Collection
    134
- Data Collection
  135
  - Setting up the Workspace
    136
  - Calculating Compensation
    139
  - Data Recording and Analysis
    141
- Data Recording and Analysis
  142
  - Setting up the Experiment
    143
  - Setting up the Global Worksheet
    144
  - Recording Data
    146
  - Analyzing Data
    147
  - Performing Batch Analysis
    150
  - Sample QC Log
    150
- Sample QC Log
  151
Chapter 5: Sorting

153
- Setting up for Sorting
  154
  - Setting up for Bulk Sorting
    155
- Determining the Drop Delay
  158
  - Setting up the Experiment
    158
  - Sorting Beads to Determine the Drop Delay
    159
- Determining the Drop Delay - Automatic Method
  162
  - Overview of Auto Drop Delay
    162
  - Using Auto Drop Delay
    163
- Sorting
  164
  - Setting up the Experiment
    165
  - Starting and Monitoring the Sort
    166
- Setting up for Sorting into a Plate or Slide
  169
  - Installing the Sorting Hardware
    169
  - Setting up the Stream
    171
  - Creating a Custom Device
    174
Chapter 6: Shutdown and Maintenance

177
- Daily Shutdown
  178
  - Fluidics Shutdown
    178
  - External Cleaning
    180
- Scheduled Maintenance
  181
  - Internal Cleaning
    181
  - Purging Filters
    198
  - Changing Fluid Filters
    199
  - Changing the Sample Lines
    200
  - Changing the Air Filters
    203
  - Changing the Bubble Filter
    203
- Unscheduled Maintenance
  205
  - Changing the Nozzle
    205
  - Adjusting the Nozzle Orifice
    207
  - Cleaning the Nozzle
    209
  - Cleaning the Camera Windows
    211
  - Cleaning the Optical Filters
    213
  - Purging the Bubble Filter
    214
Chapter 7: Troubleshooting

219
- Troubleshooting the Stream
  220
- Troubleshooting the Breakoff
  224
- Sorting Troubleshooting
  225
- Acquisition Troubleshooting
  230
- Fluidics Troubleshooting
  237
- Electronics Troubleshooting
  238
Appendix A: Supplies and Options

239
- Cytometer Supplies
  240
  - Optical Components
    240
  - Accessory Kit
    242
  - Other Replacement Parts
    244
  - Laser Specifications
    245
- Consumables
  246
  - Cytometer Setup Particles
    246
  - Reagents
    247
  - Labware
    248
- Cytometer Options
  249
- Application Options
  250
  - Two-Laser System
    250
  - Three-Laser System
    251
Appendix B: Application Settings

257

Advertisement

BD FACSAria Quick Manual

BD FACSAria Quick Manual (5 pages)

Brand: BD | Category: Measuring Instruments | Size: 0 MB

Table of Contents

BD FACSAria Startup, Qc & Shutdown Procedure

BD FACSAria Startup, Qc & Shutdown Procedure (9 pages)

Brand: BD | Category: Medical Equipment | Size: 1 MB

Advertisement

Advertisement

Related Products

BD Categories

Medical Equipment Laboratory Equipment Measuring Instruments

Refrigerator

Refrigerator Saw

More BD Manuals