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FACSCanto
BD FACSCanto Manuals
Manuals and User Guides for BD FACSCanto. We have
2
BD FACSCanto manuals available for free PDF download: User Manual, Reference Manual
BD FACSCanto User Manual (184 pages)
High Throughput Sampler
Brand:
BD
| Category:
Laboratory Equipment
| Size: 6 MB
Table of Contents
Table of Contents
5
About this Guide
9
Conventions
10
Technical Assistance
11
Chapter 1: Introduction
13
HTS Hardware Overview
14
Components
16
Cytometer Connections
19
Cytometer Interface Panel
19
Sample Coupler
21
Sample Processing
23
Chapter 2: BD Facsdiva Software Overview
27
Workspace Components
28
Plate Window
32
Setup View Components
33
Setup View Functions
43
Analysis View Components
48
Analysis View Functions
51
Chapter 3: Running Samples
55
Starting up
56
Setting up for Plate-Based Acquisition
59
Creating Experiments
64
Creating a Folder and an Experiment
64
Setting up the Plate
67
Setting up the Worksheet
74
Exporting a Plate as a Template
76
Assigning Keywords
77
Preparing for Acquisition
78
Optimizing Cytometer and HTS Settings
78
Performing Compensation
81
Acquiring Data
82
Pausing the BD High Throughput Sampler
83
Stopping the BD High Throughput Sampler
84
Exporting an Experiment as a Template
84
Analyzing Data
86
Displaying Keywords
86
Performing Data Analysis
90
Maintaining Data
93
Returning to Tube-Based Acquisition
94
Returning to Tube-Based Acquisition on the BD LSR II
94
Returning to Tube-Based Acquisition on the BD Facscanto
97
Shutting down
100
Quitting the Software
101
Chapter 4: Maintenance
103
Daily Maintenance
104
Daily Cleaning
104
Cytometer Inspection and Servicing
109
Monthly Maintenance
111
Monthly Cleaning
111
Surface Inspection and Cleaning
114
Periodic Maintenance
114
Replacing the Sample Injection Tubing
116
Replacing the HTS Sheath Filter
119
Cleaning the Air Filter
121
Replacing the Sample Coupler and Tubing
123
Replacing a Pump Syringe
125
Replacing the Probe
129
Replacing Quick-Connector O-Rings
130
Replacing SIT Protector and O-Ring (BD LSR II Only)
130
Placing HTS Unit into Long-Term Storage
132
Unscheduled Maintenance
136
Homing the Sample Probe
136
Priming the HTS
138
Performing a Motion Test
138
Verifying the Sample Probe Position
140
Reinitializing the HTS
142
Inspecting Thumbscrew Fittings
142
Inspecting Hexagonal Fittings
143
Declogging the SIT
143
Chapter 5: Troubleshooting
145
HTS Troubleshooting
146
Acquisition Troubleshooting
147
BD Facsdiva Troubleshooting
149
Appendix A: Consumables and Replacement Parts
151
Cytometer Supplies
152
Maintenance Log
154
Appendix B: Depot Repair Procedures
157
Depot Repair Overview
158
Removing the HTS Unit
159
Cleaning the HTS Unit
162
Decontaminating the Fluidics Manually
163
Decontaminating External Surfaces
167
Unpacking the Replacement Unit
169
Lifting Heavy Objects
169
Unpacking the HTS Unit
170
Installing the HTS Unit
170
Packing the Unit for Shipping
175
Placing the HTS into Its Shipping Container
175
Index
177
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BD FACSCanto Reference Manual (144 pages)
Flow Cytometer
Brand:
BD
| Category:
Laboratory Equipment
| Size: 3 MB
Table of Contents
Table of Contents
5
About this Manual
9
Conventions
10
Technical Assistance
11
Chapter 1: Introduction
13
Intended Use
14
System Components
14
Flow Cytometer
15
Fluidics Cart
25
Computer Workstation
28
BD FACS Loader (Optional)
28
Barcode Reader (Optional)
30
System Requirements
31
Chapter 2: BD FACS Loader Option
33
Loader Components
34
Using the Loader
36
Changing Operational Modes
39
Maintaining the Loader
43
Cleaning External Surfaces
43
Chapter 3: Barcode Reader Option
45
Installing and Using the Barcode Reader
46
Cleaning the Barcode Reader
48
1D Barcode Symbologies
49
2D Barcode Symbologies
50
Chapter 4: Maintenance
51
Scheduled Maintenance
52
Emptying the Waste Container
54
Purging the Fluidics Filters
57
Decontaminating the Fluidics System (Long Clean)
59
Replacing the Air Filter
60
Replacing Fluidics Filters
63
Unscheduled Maintenance
66
Changing a Cubitainer
68
Removing an Air Lock
72
Cleaning External Surfaces
75
Removing Bubbles from the Flow Cell
76
Cleaning the Flow Cell
77
Purging the Bubble Filter
78
Decontaminating the Fluidics System for Storage
80
Replacing the Bal Seal
80
Resetting the Cytometer Circuit Breaker
84
Reconnecting the Ethernet and RS232 Cables
85
Reconnecting the Fluidics Cart Tubing
85
Replacing the Fluidics Level Sensors
88
Replacing the Fluidics Cart Fuses
91
Chapter 5: Troubleshooting
95
Cytometer Troubleshooting
96
Fluidics Cart Troubleshooting
101
Loader Troubleshooting
102
Appendix A: Technical Overview
105
Flow Cytometry
106
Fluidics System
106
Optics System
109
Light Scatter
111
Fluorescence
112
Optical Filters
113
Detectors
115
Detector Arrays
115
Spillover
118
Electronics System
120
Pulses
121
Pulse Measurements
122
Appendix B: Supplies and Replacement Parts
127
Cytometer Supplies
128
Installation Kit
128
Other Replacement Parts
129
Bar Code Reader Parts
129
Consumables
130
Cytometer Setup
130
Reagents
130
Labware
131
Appendix C: Technical Specifications
133
Cytometer Specifications
134
Environment
135
Performance
135
Optics
135
Fluidics
137
Signal Processing
137
Fluidics Cart Specifications
138
Capacity
138
Loader Specifications
139
Index
141
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