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MRC PCR-300 Operation Manual
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MRC PCR-300 Operation Manual

Thermal cycler 0.2ml x 64
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Operation Manual for
Thermal Cycler 0.2ml x 64
PCR-300
PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION
3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529
mrc@mrclab.com
MRC.VER.01-9.11

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Summary of Contents for MRC PCR-300

  • Page 1 Operation Manual for Thermal Cycler 0.2ml x 64 PCR-300 PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION 3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 mrc@mrclab.com MRC.VER.01-9.11...
  • Page 2 PERFACE Thank you for purchasing PCR-300 Thermal Cycler. This manual describes function and operation procedure of the instrument. To use the instrument correctly, read this manual carefully before operating the instrument. Please keep this manual for reference in case of future trouble.
  • Page 3 Important Important Note Note Important Important Note Note ★ ★ ★ ★ This This This This instrument instrument instrument instrument only only only only use use in in in in scientific scientific scientific scientific research, research, research, research, strictly strictly strictly strictly prohibit prohibit...
  • Page 4 power line should be free of heavy objects during the instrument’s operation. Keep the power line away from the place where people gather regularly. While connect or disconnect the power line, you should hold the 3-pin plug with your hand. Insert the plug thoroughly to ensure good contact between the plug and socket.
  • Page 5 3. 3. 3. 3. Maintenance Maintenance Maintenance Maintenance The conical holes over the block and the hotlid should be cleaned termly with soaked cotton swab with a little absolute alcohol. That ensures sufficient contact and thus good heat conduction between each conical hole and the tube inside it. In case it is smeared, the surface of the instrument may be scrubbed with a piece of soft cloth with suitable detergent.
  • Page 6 CONTENTS CONTENTS CONTENTS CONTENTS CHAPTER CHAPTER CHAPTER 1 1 1 1 INTRODUCTION INTRODUCTION INTRODUCTION …………………………………………………………………………… …………………………………………………………………………… CHAPTER INTRODUCTION …………………………………………………………………………… …………………………………………………………………………… 1 1 1 1 1 1 1 1 PCR Technique Technique ……………………………………………………………………………………… ……………………………………………………………………………………… PCR Technique Technique……………………………………………………………………………………… ……………………………………………………………………………………… 1 1 1 1 2 2 2 2 PCR Application Application...
  • Page 7 2 2 2 2 Access Access Access Access an an an an PCR PCR File File File File……………………………………………………………………………… ……………………………………………………………………………… ……………………………………………………………………………… ……………………………………………………………………………… 15 15 15 15 3 3 3 3 Modify Modify Modify Modify an an an an PCR File File ………………………………………………………………………………...

  • Page 8: Pcr Pcr

    CHAPTER CHAPTER CHAPTER 1 1 1 1 INTRODUCTION INTRODUCTION INTRODUCTION CHAPTER INTRODUCTION 1. 1. 1. 1. PCR PCR Technique Technique Technique Technique Polymerase Chain Reaction (PCR) is a technique which is used to amplify the number of copies of a specific region of DNA developed in middle of 80s. There are many distinct merits in the PCR technology, such as specialization, sensitivity, high productivity, celerity, convenience, good reproductively, automatization and so on.
  • Page 9 Conditions Conditions Conditions Ambient temperature : -20℃～+55℃ Relative humidity : ≤80% 3. 3. 3. 3. Basic Basic Basic Parameters Parameters Basic Parameters Parameters PCR-300 Model Parameters Sample Number Tube Volume(ml) Size(mm) 370×248.5×222 (L×W×H) Weight(kg) Fuse 250V 2.5A Φ5×20 Temperature range:0℃～99℃...
  • Page 10 Pause of program execution Stop of program execution Auto-restart in case of power failure Sound alarm/indicator light Estimating total program execution time and remaining program execution time Date and time display and calibration Protecting the device from further damage and alarming in case of failure 5.
  • Page 11 Operation Operation Panel Panel Diagram Diagram Diagram： ： ： ： Operation Operation Panel Panel Diagram Functions Functions Functions： ： ： ： Key Functions Digit key/time display and calibration; Moving up, every time when pressed during file editing, listing or modifying, display the setting state of the previous screen. Moving down,every time...
  • Page 12 Page up key, every time when pressed during file editing, listing or modifying, display the setting position of the segment #1 of previous unit. Page down key, every time when pressed during file editing, listing or modifying, display the setting position of the segment #1 of next unit.
  • Page 13 Stop the current program when pressed once during program execution. Run key, press run key to run after open and select the target file. press this key to run the lately operation file when in idle state. Enter key, accepting the current setting and displaying the next screen when pressed during file editing, listing, modifying, or deleting.
  • Page 14 second: Seize the free handle and tote it upward till the module leave Completely from the shell. (Note: Note: Note: Note: There is a outlet in the module right. It will lead to the left and the right of the force not the same,but must keep parallel to take out the module).
  • Page 15 2. 2. 2. 2. Power-On Power-On Power-On Power-On Turn on the power, the instrument will beep, that means the power have been supplied, and the LCD will display ‘Start Waiting…’(see fig.1),the yellow power indicator lights, and the device will have self-testing, after the self-testing is passed, the device is ready for operation such as running, accessing, modifying, or deleting a PCR amplification file;...

  • Page 16: File File

    Edit Edit Edit a a a a New Amplification Amplification File File 1. Edit New PCR PCR Amplification Amplification File File Cyc. File Unit Seg. In idle state, press ‘Edit’ to enter the editing state directly. Time Setting the program unit #1: Firstly, input the number of cycles, when cursor flickering at cycle number input Press 'Edit' in...
  • Page 17 Set the time of a certain time segment to zero value will terminate segment #3 to zero Press ‘ ’ the setting of program unit #1,press value means this ‘Enter’ key to confirm. segment terminate segment. NOTICE: NOTICE: NOTICE: NOTICE: Every program may include Cyc.
  • Page 18 decrements). example, segment temperature increases by 0.3℃, and the time increases by 3sec.,press ‘ENTER’ key to confirm. Input the temperature Repeat these steps until temperature and time increments / and time of all the segments of decrements of segment program unit #1 is embellished. #1,press 'Enter' key to confirm.
  • Page 19 If the setting of program unit #1 is finished, set the time of segment #3 to zero, press ‘Enter’ key to confirm. Cyc. File Unit Seg. SetTe NOTICE: NOTICE: NOTICE: NOTICE: Every program may include Time as many as nine segments,Any segment,numbered greater than or equal to 1,could be a terminal segment if its time setting is zero.
  • Page 20 NOTICE: NOTICE: NOTICE: NOTICE: number Cyc. File Unit Seg. program units is 9 .Any program unit, Set Te numbered greater than or equal to 1, could be a terminal to quit the program setting state unit if its cyc. Time value is zero.
  • Page 22 Access Access File File Access Access PCR PCR File File Cyc. File Unit Seg. In idle state, press ‘open’ key, the screen will display the saved file’s name and storage dates. Time Press 'Open' key Press ‘Esc’ to open filelist. Press ‘...
  • Page 23 2. 2. 2. 2. Modify Modify Modify Modify an an an an PCR File File PCR File File File Unit Cyc. Seg. In idle state, press ‘Open’ key and via the ’ ‘key and ‘ ’key to select the target file(for example file 07). Date After select target file, press ‘Enter’...
  • Page 24 3. 3. 3. 3. Delete Delete Delete an an an an PCR File File Delete PCR File File Cyc. File Unit Seg. In idle state, press ‘open’ key and via the ’ ‘key or ’ ‘key to select the target file(for example file 07). Time When you select the file, press ‘Del’...
  • Page 25 4. 4. 4. 4. Run Run a a a a PCR Program Program PCR Program Program Cyc. File Unit Seg. In idle state, press ‘Open’ key and via the’ ‘key or ’ ’key to select the Date target file(for example file 07). Selected the file, press ‘Run’...
  • Page 26 Accept default Press ‘ ‘ The screen asks whether the hot lid is value and press to be used, and the screen displays 'Enter' key. flickering ‘Yes’, press’ ’key and’ ’ key to choose ‘Yes’ or ’No’. Cyc. File Unit Seg.
  • Page 27 Cyc. After the normal program execution, if File Unit Seg. the soak mode is selected during the file setting, the screen will display the current temperature and delay from the Time start of this mode(for example Fig 4). If you want to stop this mode at this time, Fig.4 just press the ‘Stop’...
  • Page 28 5. 5. 5. 5. translate translate translate display display during during program program execution execution translate the the display display during during program program execution execution Display during cycling program execution Display Display Display during during during the the cycling cycling cycling program program...
  • Page 29 access access calibrate calibrate internal internal clock clock 6. 6. 6. 6. access access and and calibrate calibrate the the internal internal clock clock The format of data displays is’ ’ from left is year, month and day in sequence, the format of time is‘...

  • Page 30: Maintenance Maintenance

    Chapter Chapter Chapter 5 5 5 5 Maintenance Maintenance Maintenance Troubleshooting Troubleshooting Chapter Maintenance and and Troubleshooting Troubleshooting 1. 1. 1. 1. Maintenance Maintenance Maintenance Maintenance 1 1 1 1 Regular Regular Clean Clean Regular Regular Clean Clean mild soap solution. A．...
  • Page 31 2. 2. 2. 2. Failure Failure Analysis Troubleshooting Failure Failure Analysis Analysis Analysis and and Troubleshooting Troubleshooting Troubleshooting Phenomenon Possible cause Processing Processing P P P P rocedure rocedure Phenomenon Phenomenon Phenomenon Possible Possible Possible cause cause cause Processing Processing rocedure rocedure Disconnected power...
  • Page 32 3. 3. 3. 3. Phenomenon Phenomenon Phenomenon of of of of Failure Failure Failure Phenomenon Failure Failure and and Its Its Failure Failure Failure No. Error Error Error Error No. Failure Failure Failure Failure reason reason reason reason Processing Processing Processing Processing procedure procedure...
  • Page 33 APPENDIX APPENDIX APPENDIX 1 1 1 1 CONNECTION CONNECTION CONNECTION DIAGRAM DIAGRAM APPENDIX CONNECTION DIAGRAM DIAGRAM （This figure only for reference, it is subject to change without prior notice） Input Power AC220V 250VA 50Hz Switch AC250V 10A Fuse AC250V 2.5A Block Hotlid ∅5×20...