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Summary of Contents for MRC PCR-IF-8C
- Page 1 Operation Manual PCR-IF-8C Isothermal Fluorescence PCR PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 mrc@mrclab.com MRC.2.22...
- Page 3 Opening check Please check the instrument and accessories with the packing list when you first open the packing case. If you find any wrong or missing, please contact the distributor or the manufacturer. .Company: MRC CO., LTD...
- Page 4 Important Guidelines important safety operation Please have a comprehensive understanding of how to use before the operation. Therefore, read this manual carefully before using it. Any improper operation before reading the manual is forbidden, otherwise instrument may cause injured or electric shock. Please read below safety tips and guidelines, and take precautions to ensure safety.
- Page 5 The sample holder and heat lid are hot and may cause the scald when the instrument is working, please make sure the temperature is cool when you touching them. The instrument should be kept in a place with low humidity and dust.
- Page 6 Clean the inspection hole and disinfect the instrument with ultraviolet light before transport please. Retain the original package for transport using to avoid damage during transportation. Warning label Warning: Hot surface, risk of scald.
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Page 7: Table Of Contents
Contents Chapter 1 Introduction ..................... 1 Chapter 2 Features ....................2 Chapter 3 Accessories List ..................4 Chapter 4 System structure and accessories ............5 Chapter 5 Operation Guide ..................8 Chapter 6 Fault analysis and solutions ..............17 Chapter 7 Maintenance, storage and transportation ..........17 Chapter 8 Abbreviations and Tags ................ -
Page 8: Chapter 1 Introduction
Operation Manual Chapter 1 Introduction Chapter 1 Introduction PCR-IF-8C Isothermal Fluorescence PCR is contain temperature control block, fordoptical path detection system, heating lid and analysis software. It is use d cannisothermal amplification and fluorescence real-time detection of samples, a s widelyimake quantitative and qualitative analysis of the amplification result. It i,gused in rapid detection of pathogenic microorganisms(viruses, bacteria, fun mycoplasma, parasites etc.), food hygiene inspection, environmental monitoring,... -
Page 9: Chapter 2 Features
Power supply: Input AC 100~240V, 50Hz~60Hz; Output DC15V, 8A; Ambient: Dry, clean, flat and firm horizontal surface, Away from direct sunlight, mechanical vibration and strong magnetic field interference. 2.2. Basic Parameters and Performance PCR-IF-8C Product model 0.2mL–8wells Capacity (Strip of 8 tubes) Technical 10-150μL... - Page 10 Operation Manual Chapter 2 Features Analysis of melting curve and melting Fusion function peak 1. positive/negative; 2. peak time; Result display mode Tm value Optional software PC software Software upgrade/Picture updated U disk upgrade/ U disk updated...
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Page 11: Chapter 3 Accessories List
After opening the package, please check each component against the standard configuration form below and check for missing or damaged. Name Spec/Model Unit Remark PCR-IF-8C Mainframe The plug form is selected Power cord according to the order Power adapter Operation manual... -
Page 12: Chapter 4 System Structure And Accessories
Operation Manual Chapter 4 System structure and accessories Chapter 4 System structure and accessories This chapter mainly introduces the structure of the instrument, kit installation as well as the preparatory work before starting up. Please have a comprehensive understanding about the instrument before the first time using. 4.1. - Page 13 Operation Manual Chapter 4 System structure and accessories 4.3. Power adapter 4.4. USB cable, USB flash disk 4.5. Thermal printer Disclaimer: the above picture is for indication only, subject to actual delivery.
- Page 14 Operation Manual Chapter 4 System structure and accessories 4.6. Applicable consumables Specification Applicable situation Non-optical/100ul optical PCR tube × 200uloptical PCR tube-Dome top single × tube/Dome top strip of 8 tubes 200uloptical PCR tube-Flat top single tube √ 200uloptical PCR tube-Flat top strip of 8 tubes √...
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Page 15: Chapter 5 Operation Guide
Operation Manual Chapter 5 Operation Guide Chapter 5 Operation Guide 5.1. Instrument start Connect the power adapter, power on switch on the side of the instrument. The instrument will automatically load the detection system, and the progress dot on the screen will display the loading progress in real time. ... - Page 16 Operation Manual Chapter 5 Operation Guide 5.3 Experiment setup and operation There are two ways for users to experiment, that are fast start experiment and new program experiment. Way 1 Fast start experiment The prerequisite for this way: The test interface has successfully stored the program file of the new setting or the program has been stored and run.
- Page 17 Operation Manual Chapter 5 Operation Guide 2) Negative and positive setting Click the default peak judgment blue button to set result judgment. Del button, cancel the judgment. Add button, add the judgment, and set the time, can combine a variety of results.
- Page 18 Operation Manual Chapter 5 Operation Guide 5.4. Program Running The program interface has five function interfaces: Running program, real-time temperature, amplification curve, anneal curve and results. In order to facilitate the user to view the data during the experiment, the switch operation can be carried out quickly.
- Page 19 Operation Manual Chapter 5 Operation Guide 3) Amplification curve It used to display the reagent amplification curves during the experiment in real time. The x-coordinate is time(Unit: min) and the y-coordinate is fluorescence intensity. Coordinate will be adjusted automatically. ...
- Page 20 Operation Manual Chapter 5 Operation Guide 5.5 Data review and management Click the <Data> interface on the right of <Test>. Rules for data storage: 1) Type of program file, which is classification. 2) Sort the data files in reverse order according to the time they have been saved, which is time sort.
- Page 21 Operation Manual Chapter 5 Operation Guide 5.6 Instrument function Click <Setting>, the interface included Experiment program new/manage, instrument setting and information, system setting function, etc. As shown in figure. Experiment setting function. please read section 5.3 Sample Name Library ...
- Page 22 Operation Manual Chapter 5 Operation Guide Color Management Enter to color management interface, select the sample graph and choose the color for it. Then the sample graph color will change. Save: save new setting color. Defult: Restore the original color of the instrument.
- Page 23 Operation Manual Chapter 5 Operation Guide System Setting System setting include Battery, Lid Temperature, Time, Screen, Update, Maintain. Maintain It is a professional option for maintenance personnel or instrument developers to maintain, and calibrate instruments. Please refer to the instrument maintenance manual for details and operation.
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Page 24: Chapter 6 Fault Analysis And Solutions
Operation Manual Chapter 6 Fault analysis and solutions Chapter 6 Fault analysis and solutions Faults phenomenon and interface prompts that may occur during the use of the instrument. Refer to this list for solutions to identify and resolve faults with your detector. - Page 25 Operation Manual Chapter 6 Fault analysis and solutions Software fault alarm table Fault type Name Alarm content Memory chip read failed. Memory read failed. Touch screen Touch chip read failed. Touch chip read failed. Acquisition Capture chip read failed. Capture chip read failed. board Module temperature sensor Module temperature sensor error.
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Page 26: Chapter 7 Maintenance, Storage And Transportation
Operation Manual Chapter 7 Maintenance, storage and transportation Chapter 7 Maintenance, storage and transportation During the use of the instrument, timely and regular maintenance is required to keep the instrument in a good state of use. Instrument cleaning Surface cleaning ... - Page 27 Operation Manual Chapter 7 Maintenance, storage and transportation Waste treatment After each experiment, there's a lot of amplification in the tube. To avoid contaminating laboratory and instruments, it should be dealt with according to regulations as soon as possible. Do not open the tube when removing it from the reaction module, otherwise the ...
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Page 28: Chapter 8 Abbreviations And Tags
Operation Manual Chapter 8 Abbreviations and Tags Chapter 8 Abbreviations and Tags Abbreviations The following Abbreviations are for reference and will appear in this operation manual. Ampere Alternating current Direct current Volt Hertz Watt Universal Serial BUS Kilogram Millimeter μL microliter ℃...
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