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Summary of Contents for Knauer BlueShadow Detector 50D
- Page 1 Detector 50D Instructions HPLC HPLC Document no. V7659...
- Page 2 Support: Do you have questions about the installation or the operation of your instrument or software? International Support: Contact your local KNAUER partner for support: www.knauer.net/en/Support/Distributors-worldwide Support in Germany (Austria & Switzerland on case-to-case basis): Phone: +49 30 809727-111 (workdays 9-17h CET)
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Page 3: Table Of Contents
Symbols and signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 BlueShadow Detector 50D Instructions V7659... - Page 4 Program menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 BlueShadow Detector 50D Instructions V7659...
- Page 5 Storing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60 BlueShadow Detector 50D Instructions V7659...
- Page 6 Titanium, titanium alloy (TiA16V4) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 BlueShadow Detector 50D Instructions V7659...
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Page 7: General
These operating instructions are an integral part of the device . It must be kept in the immediate vicinity of the device and accessible to the user at all times . You can download these and other instructions from the KNAUER web- site: www .knauer .net/library . Signal words Possible dangers related to the device are distinguished in personal and material damages . -
Page 8: Legal Information
For information on warranty please refer to our general terms and condi- tions on the website: www .knauer .net/terms 1.4.4 Declaration of conformity The declaration of conformity is enclosed as a separate document with the product and can be obtained online: www .knauer .net/en/Support/Declarations-of-conformity BlueShadow Detector 50D Instructions V7659... -
Page 9: Basic Safety Instructions
. Operation in potentially explosive areas without special and additio- ƒ nal explosion protection . Contact the KNAUER Customer Support for more information . User qualification The users are qualified to handle the device if all of the following points apply: They have at least a basic knowledge of liquid chromatography . -
Page 10: Operator Responsibility
Protective gloves in accordance with the prevailing ambient conditions ƒ and used solvents (e .g . heat, cold, protection against chemicals) Lab coat ƒ Personalised protective afety equipment which is specified in the parti- ƒ cular laboratory BlueShadow Detector 50D Instructions V7659... -
Page 11: Working With Solvents
In case of leakage: Switch off the system . Only take the device into ƒ operation if the cause of the leak has been resolved (see „8 Mainte- nance and care“, p . 56) . BlueShadow Detector 50D Instructions V7659... -
Page 12: Specific Environments
ƒ system in order to avoid dead volumes, not exactly fitting connections and spreading contamination . Safety features: The device may only be opened by the KNAUER Cus- ƒ tomer Support of KNAUER or any company authorized by KNAUER (see „1 .4 .1 Liability limitation“, p . 6) . -
Page 13: Service Request Form And Decontamination Report
Devices which are shipped without the completed document “Service request form and decontamination report” will not be repaired . If you would like to return a device to KNAUER, make sure to enclose the com- pleted document: www .knauer .net/servicerequest . -
Page 14: Product Information
Flexible options for use in the entire field of LC applications due to ƒ a comprehensive range of flow cells for the detectors, from nano HPLC cells with flow rates ≈ 100 nl/min to preparative flow cells with 10 l/min . BlueShadow Detector 50D Instructions V7659... -
Page 15: Scope Of Delivery
Product information Scope of delivery The following items should be included in the scope of delivery: BlueShadow Detector 50D with test cell ƒ BlueShadow Detector 50D accessory kit ƒ Power cable ƒ Transport protection ƒ Valid documents: BlueShadow Detector 50D Instructions V7654 ƒ... -
Page 16: Rear View
Declaration of Conformity . Testing seals in Canada and the USA at nationally recognized testing centers (NRTL) . The certified device or system has successfully passed the qual- ity and security tests . BlueShadow Detector 50D Instructions V7659... -
Page 17: Installation And Initial Startup
. Allow the device to accli- mate for 3 h, before it is connected to the power supply and taken into operation . BlueShadow Detector 50D Instructions V7659... -
Page 18: Space Requirements
Country-specific plugs: Before switching on the device, check whether ƒ the supplied plug is approved for your country . Overview of the de- vice- and country-specific plug types from KNAUER: www .knauer .net/plugs . Power strips: If several devices are connected to one power strip, al- ƒ... -
Page 19: Inserting The Flow Cell
Inserting the flow cell screws back into the cover plate and tighten . 7. Connect the incoming and outgoing capillaries at the inlet and out let of the measuring cell . Next step Connect the capillaries . BlueShadow Detector 50D Instructions V7659... -
Page 20: Changing The Optical Path Length Of The Preparative Flow Cell
1 .25 mm . To further shorten down to 0 .5 mm, the PEEK spacer on the other cell side must also be removed . Fig. 4 Shortening the optical path length BlueShadow Detector 50D Instructions V7659... - Page 21 0 .75 mm . The PTFE seal does not need to be repla- ced when the path length is changed . Next step Insert the flow cell into the detector . BlueShadow Detector 50D Instructions V7659...
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Page 22: Connecting Capillaries
3. Manually, screw together the capillary and the flow cell . Tighten the fitting with the wrench . Fig. 7 Capillary and flow cell Result Result Capillaries have been connected and the detector is ready for operation . BlueShadow Detector 50D Instructions V7659... -
Page 23: Computer Control
Fig. 8: Connectors on rear side Note: HPLC devices from KNAUER only work with IP addresses, which have been assigned by IPv4 . IPv6 is not supported . This chapter describes how to set up a chromatography system in a local area network (LAN) and how a network administrator can integrate this LAN into your company network . -
Page 24: Configuring Lan Settings
. To connect the router to a LAN, an additional patch cable is required, which is not supplied within the scope of delivery . Legend Devices Router LAN ports WAN/Internet connection Computer Fig. 9: Connect devices with LAN BlueShadow Detector 50D Instructions V7659... -
Page 25: Configuring The Router
Set the router properties (see section „4 .6 .4“) . 4.6.4 Configuring the router The router is preset at the factory . Information about address, user name and password is noted in the router manual: www .knauer .net/router . Process Procedure 1. -
Page 26: Controlling Several Systems Separately In Lan
. Process 1. Find out port number and change it on the device . Procedure 2. Enter the port number in the chromatography software . Result The connection is established . BlueShadow Detector 50D Instructions V7659... -
Page 27: Remote Control
. Permanent state signal: passive = open relay contact ƒ activ = closed relay contact ƒ Pulse: Closed relay contact for at least 1000 ms ƒ Permissible load of the relay contact: 1 A/ 30 V BlueShadow Detector 50D Instructions V7659... - Page 28 250 mA fuse . This output can be used to operate a fraction valve . External λ Allows external analog control of the detector wave- length in single channel mode . . The control voltage must be applied against AGND . BlueShadow Detector 50D Instructions V7659...
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Page 29: Connecting The Pin Header
Turn off the device before connecting cables . Pull the power plug . Electronic defect Electrostatic discharge can destroy the electronics . Wear a protective bracelet against electrostatic discharge and ground . Fig. 11: Depressor tool BlueShadow Detector 50D Instructions V7659... -
Page 30: Analog Control
1. Insert the plug into the male connector at the rear panel . 2. Plug the other end into an electric socket . 3. Switch on the device at the power switch Fig. 12 Power switch with male con- nector BlueShadow Detector 50D Instructions V7659... -
Page 31: Operation
If the device is part of an HPLC system, you should also note the following: The network connection to the router is established ƒ The chromatography software has been installed by KNAUER or a ƒ com pany authorized by KNAUER . The capillaries have been connected . -
Page 32: Led Status
The keypad consists of 15 buttons, which allow to operate the device . Function Explanation Numeric pad Activation of numeric ƒ pad by pressing the confirmation button at input fields . Changing values . ƒ BlueShadow Detector 50D Instructions V7659... -
Page 33: Switch-On And Self-Test
2. After turning the device on, the detector displays the following, consecutive status messages: „Initialize“ - self test of electronic com- ƒ ponents „Lamp heating“ - preheating and ignition ƒ of the D2 lamp „Calibrating“ - optical calibration ƒ BlueShadow Detector 50D Instructions V7659... -
Page 34: Selecting A Wavelength
The absorption ratio of the measurement can thus be generated to check the chromatographic separation quality at two wavelengths . The ratio values are displayed by the symbols 1/2 or 2/1 at the decimal point of the absorption value . BlueShadow Detector 50D Instructions V7659... -
Page 35: Menu Structure
. If you want to use the numeric pad, simply press the confirmation button to activate numeric pad . A decimal point is available via the standby key in case the numeric pad is activated . BlueShadow Detector 50D Instructions V7659... -
Page 36: Menu Structure: Overview
Operation 5.4.2 Menu structure: overview Fig. 15: Menu structure: overview BlueShadow Detector 50D Instructions V7659... -
Page 37: Device Setting Parameters
10 s) to smooth the analog out- put signal . The larger the value of the selected time constant, the stronger the applied signal smoothing . Date/Time Change real time clock date and time of the detector . BlueShadow Detector 50D Instructions V7659... - Page 38 Via Integrator ch . the output ƒ channel for the scan can be selected . The parameter Speed defines ƒ the rate (wavelength/time) at which the spectral data are send as an analog signal to the integrator port . BlueShadow Detector 50D Instructions V7659...
- Page 39 . For the recording of possi- ble negative absorption values, an according offset has to be predifined, since the integrator is providing positive voltage le- vels only . Otherwise all absorp- tion values/voltage levels below zero will be clipped . BlueShadow Detector 50D Instructions V7659...
- Page 40 . 28 for details on permissi- ble signal voltages, loads, etc . Display setup Adapt Brightness and Contrast of the display, as well as the brightness of the status LED via LED power . BlueShadow Detector 50D Instructions V7659...
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Page 41: Glp Menu
(H-beta spectral line) . Firmware Currently installed Firmware Ver- Version/Display sions for detector and detector firmware display . Install. Date Display of the initial installation Service Date and last service . BlueShadow Detector 50D Instructions V7659... -
Page 42: Program Menu
6. To create a new line, set the start time for the new program step using the vertical arrow keys or the confirmation key combined with the numeric keypad . Use the confirmation key to continue BlueShadow Detector 50D Instructions V7659... -
Page 43: Creating A Program
6. Edit wavelength and events as done before (see also step 3) . 7. Press the menu key for 2 seconds to exit the submenu . The program is automatically saved . BlueShadow Detector 50D Instructions V7659... -
Page 44: Running A Program
8. To do so, navigate the cursor to the program status symbol and use the downward key to clear the status . Also, you might press the upward key to return to hold and run mode . BlueShadow Detector 50D Instructions V7659... -
Page 45: Changing A Program
(e .g ., P01) . 4. You will be asked whether you really want to delete the program . Confirm with the confirma- tion key or deny with the menu key BlueShadow Detector 50D Instructions V7659... -
Page 46: Creating And Running A Program With Preselectable Start Time
6. Before running the program, the detector will perform a self-calibration . 7. By pressing the standby button for 2 seconds the device will immediately start to recalibrate, interrupting the standby and deactivating the wake-up program . BlueShadow Detector 50D Instructions V7659... -
Page 47: Device Links
6. Move the cursor via the horizontal arrow keys to the double arrow on the lower left corner . 7. Use the vertical arrow keys to navigate to the next line or create a new line . BlueShadow Detector 50D Instructions V7659... -
Page 48: Creating A Link
. 9. Confirm the new line number via the confirma- tion key . Now, you can edit the values of the new line, as done before . BlueShadow Detector 50D Instructions V7659... -
Page 49: Running A Link
“E” (End) . Navigate the cursor to the link status and press the upward key return to hold and run mode . 11. Use the downward key to clear the status . BlueShadow Detector 50D Instructions V7659... -
Page 50: Changing A Link
3. After pressing the confirmation key , you will be asked whether you really want to delete the selected link . 4. Confirm once more with the confirmation key or deny with the menu key BlueShadow Detector 50D Instructions V7659... -
Page 51: Scan Menu
. 3. The progress of the output will be indicated by the continuing rise in the wavelength . BlueShadow Detector 50D Instructions V7659... -
Page 52: Functionality Tests
Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge . In case of a request, the technical support of KNAUER or from a provider authorized by KNAUER performs this functionality test during the installation . -
Page 53: Troubleshooting
6. Replace the patch cable to the device with that no connection could be established . Has this been successful? ƒ 7. Make sure that the IP port of the device matches the port in the chro- matography software . BlueShadow Detector 50D Instructions V7659... -
Page 54: Possible Problems And Solutions
. The relationship Rinsing the flow cell . ƒ of the signal to the Clean the flow cell window . ƒ light path reference Exchange lamp (spectrum source) . ƒ is very low BlueShadow Detector 50D Instructions V7659... -
Page 55: System Messages
Switch the device off and on . If the system message appears again, notify Technical Support . ”Instrument remote This entry is not executable . controlled“ Quit software . ”Invalid command” Check the cable connections . Change the entry . BlueShadow Detector 50D Instructions V7659... - Page 56 Correct the time entry . exists“ ”Time table is not The device is in Standalone mode, no pro gram active“ is running . If you try to quit a non-existent pro- gram sequence, this message appears . BlueShadow Detector 50D Instructions V7659...
- Page 57 Edit the program line . empty“ ”Too many lines in Check the number of program lines . program“ A maximum of 100 program lines are possi ble . ”Wrong Line Change the entry in the program line . number“ BlueShadow Detector 50D Instructions V7659...
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Page 58: Maintenance And Care
Switch off the detector or the lamps . Maintenance contract The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a sepa rate maintenance contract: Opening the device or removing housing parts . -
Page 59: Cleaning And Caring For The Device
2. Inject it into the inlet of the flow cell and allow it to act for 5 minutes . 3. Fill the syringe with water and rinse the inlet . 4. Use a nitrogen stream to dry the flow cell . BlueShadow Detector 50D Instructions V7659... -
Page 60: Cleaning The Lens Of An Analytical Flow Cell
5. Assemble the flow cell . Make sure that the new seal ring does not interrupt the light path . 6. Using the allen screwdriver, tighten the threaded ring Result The flow cell lens is clean . BlueShadow Detector 50D Instructions V7659... -
Page 61: Cleaning The Light Guide Of A Preparative Flow Cell
Power plug has been pulled . ƒ Flow cell has been removed . ƒ Tools Tweezers ƒ Allen screwdriver, size 3 ƒ Threaded ring Cover Spacer Pressure bushing Light guide with seal ring Fig. 17: Preparative flow cell BlueShadow Detector 50D Instructions V7659... - Page 62 The flow cell lens is clean . Note: If the cleaning of the light guide does not have the desired effect, the light guide has to be replaced . Next steps Mount the flow cell onto the detector . BlueShadow Detector 50D Instructions V7659...
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Page 63: Replacing The Flow Cell
. 7. Screw the knurled-head screws back into the cover Fig. 18 Replacing the flow cell plate and tighten . Result The flow cell is assembled . Next step Connect the capillaries . BlueShadow Detector 50D Instructions V7659... -
Page 64: Transport And Storage
. Transporting the device Carefully prepare the device for transport or storage . If you want to return your device to KNAUER for repairs, enclose the Service Request Form which can be downloaded from our website . Device data For a secure transport, note the weight and dimensions of the device (see „Technical data“... -
Page 65: Disposal
160214 . 10.2 WEEE registration number KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR) . The number classifies to category 8 and 9, which, among others, comprises laboratory equipment . -
Page 66: Technical Data
10 Hz (RS-232) 1 channel ƒ Inputs Error (IN) ƒ Start (IN) ƒ Autozero ƒ 0–10 V Analog IN ƒ Outputs Events 1–3 ƒ +5 V ƒ 24 V Valve ƒ Analog outputs 2 × 0–5 V scalable, 20 bit, offset adjustable BlueShadow Detector 50D Instructions V7659... -
Page 67: General
Line voltage fluctuations do not exceed ± 10 % of normal voltage . Overvoltage category II: Energy consumers are supplied by a fixed device . Permitted pollution degree Temperature range 4-40 °C (39 .2-104 °F) Air humidity below 90 %, non-condensing Operating height max . 2000 meters above sea level BlueShadow Detector 50D Instructions V7659... -
Page 68: Chemical Compatibility Of Wetted Materials
. The following list contains information about the chemical compatibility of all wetted materials which are used in devices made by KNAUER . The data bases on a literature research on the manufacturer specifications of the materials . The wetted materials of this device are listed in the chapter “Technical data”... -
Page 69: Polyimide (Vespel®)
This amorphous perfluorinated copolymer is inert against all commonly used solvents . However, it is soluble in perfluorinated solvents like Fluo- rinert® FC-75 and FC-40, and Fomblin perfluor-polyether solvents from Ausimont . In addition, it is affected by Freon® solvents . BlueShadow Detector 50D Instructions V7659... -
Page 70: Polychlortrifluorethylene (Pctfe, Kel-F®)
Synthetic ruby is monocrystalline alumina and gets its red color by the addition of some chromium oxide . It is biocompatible and very resistant to corrosion and wear . The material is characterized by a high hardness and a high thermal conductivity . BlueShadow Detector 50D Instructions V7659... -
Page 71: Mineral Wool
Titanium has a low weight and a high hardness and stability . It stands out due to its very high chemical compatibility and biocompatibility . Titan is applied when neither stainless steel nor PEEK are usable . BlueShadow Detector 50D Instructions V7659... - Page 72 Latest KNAUER instructions online: www.knauer.net/library KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Fax: +49 30 8015010 Hegauer Weg 38 E-Mail: info@knauer.net 14163 Berlin Internet: www.knauer.net © KNAUER 2020...
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