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Summary of Contents for Knauer BlueShadow Detector 40D
- Page 1 Detector 40D Instructions HPLC HPLC Document no. V7647...
- Page 2 Support: Do you have questions about the installation or the operation of your instrument or software? International Support: Contact your local KNAUER partner for support: www.knauer.net/en/Support/Distributors-worldwide Support in Germany (Austria & Switzerland on case-to-case basis): Phone: +49 30 809727-111 (workdays 9-17h CET)
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Page 3: Table Of Contents
Symbols and signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 BlueShadow Detector 40D Instructions V7647... - Page 4 Parameters in the Device-/Lamps GLP menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 BlueShadow Detector 40D Instructions V7647...
- Page 5 Storing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 BlueShadow Detector 40D Instructions V7647...
- Page 6 Titanium, titanium alloy (TiA16V4) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 BlueShadow Detector 40D Instructions V7647...
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Page 7: General
These operating instructions are an integral part of the device . It must be kept in the immediate vicinity of the device and accessible to the user at all times . You can download these and other instructions from the KNAUER web- site: www .knauer .net/library . Signal words Possible dangers related to the device are distinguished in personal and material damages . -
Page 8: Legal Information
.knauer .net/terms 1.4.4 Warranty seal A blue or orange warranty seal is affixed to some devices . A blue seal is used by KNAUER’s Manufacturing or Customer Support ƒ for devices to be sold . After repair, service technicians attach an orange seal onto the identi- ƒ... -
Page 9: Basic Safety Instructions
. Operation in potentially explosive areas without special and additio- ƒ nal explosion protection . Contact the KNAUER Customer Support for more information . User qualification The users are qualified to handle the device if all of the following points apply: They have at least a basic knowledge of liquid chromatography . -
Page 10: Operator Responsibility
Protective gloves in accordance with the prevailing ambient conditions ƒ and used solvents (e .g . heat, cold, protection against chemicals) Lab coat ƒ Personalised protective safety equipment which is specified in the ƒ particular laboratory . BlueShadow Detector 40D Instructions V7647... -
Page 11: Working With Solvents
In case of leakage: Switch off the system . Only take the device into ƒ operation if the cause of the leak has been resolved (see „8 Mainte- nance and care“, p . 51) . BlueShadow Detector 40D Instructions V7647... -
Page 12: Specific Environments
ƒ system in order to avoid dead volumes, not exactly fitting connections and spreading contamination . Safety features: The device may only be opened by the KNAUER Cus- ƒ tomer Support of KNAUER or any company authorized by KNAUER (see „1 .4 .1 Liability limitation“, p . 2) . -
Page 13: Service Request Form And Decontamination Report
Devices which are shipped without the completed document “Service request form and decontamination report” will not be repaired . If you would like to return a device to KNAUER, make sure to enclose the com- pleted document: www .knauer .net/servicerequest . -
Page 14: Product Information
ƒ Flexible options for use in the entire field of LC applications due to a ƒ comprehensive range of flow cells for the KNAUER detectors, from nano HPLC cells with flow rates ≈ 100 nl/min to preparative flow cells with 10 l/min . -
Page 15: Views
. Legend Serial number Fan opening Power switch Mains power connection Pin header RS-232 port LAN connector Integrator output Fig. 2: Rear view BlueShadow Detector 40D Instructions V7647... -
Page 16: Symbols And Signs
Testing seals in Canada and the USA at nationally recognized testing centers (NRTL) . The certified device or system has successfully passed the qual- ity and security tests . The device complies with the product-specific requirements of the United Kingdom . BlueShadow Detector 40D Instructions V7647... -
Page 17: Installation And Initial Startup
. Allow the device to accli- mate for 3 h, before it is connected to the power supply and taken into operation . BlueShadow Detector 40D Instructions V7647... -
Page 18: Space Requirements
Country-specific plugs: Before switching on the device, check whether ƒ the supplied plug is approved for your country . Overview of the de- vice- and country-specific plug types from KNAUER: www .knauer .net/plugs . Power strips: If several devices are connected to one power strip, al- ƒ... -
Page 19: Inserting The Flow Cell
The supply configuration of this detector only includes a test cell (dum- my cell) from KNAUER, which does not have connections for solvent . It is used, for example, to check the lamp intensity, as a dirty flow cell could distort this value . -
Page 20: Changing The Optical Path Length Of The Preparative Flow Cell
1 .25 mm . To further shorten down to 0 .5 mm, the PEEK spacer on the other cell side Fig. 4: Shortening the optical must also be removed . path length BlueShadow Detector 40D Instructions V7647... - Page 21 0 .75 mm . The PTFE seal does not need to be repla- ced when the path length is changed . Next step Insert the flow cell into the detector . BlueShadow Detector 40D Instructions V7647...
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Page 22: Connecting Capillaries
Fig. 6 Capillary fitting 3. Manually, screw together the capillary and the flow cell . Tighten the fitting with the wrench . Fig. 7 Capillary and flow cell Result The capillary has been connected . BlueShadow Detector 40D Instructions V7647... -
Page 23: Computer Control
Fig. 8: Connectors on rear side Note: HPLC devices from KNAUER only work with IP addresses, which have been assigned by IPv4 . IPv6 is not supported . This chapter describes how to set up a chromatography system in a local area network (LAN) and how a network administrator can integrate this LAN into your company network . -
Page 24: Configuring Lan Settings
. To connect the router to a LAN, an additional patch cable is required, which is not supplied within the scope of delivery . Legend Devices Router LAN ports WAN/Internet connection Computer Fig. 9: Connect devices with LAN BlueShadow Detector 40D Instructions V7647... -
Page 25: Configuring The Router
Set the router properties (see section „4 .6 .4“) . 4.6.4 Configuring the router The router is preset at the factory . Information about address, user name and password is noted in the router manual: www .knauer .net/router . Process Procedure 1. -
Page 26: Integrating Lan Into The Company Network
. Process 1. Find out port number and change it on the device . Procedure 2. Enter the port number in the chromatography software . Result The connection is established . BlueShadow Detector 40D Instructions V7647... -
Page 27: Remote Control
Closed relay contact for at least 1000 ms ƒ Permissible load of the relay contact: 1 A/ 24 V DC EV 2 TTL output (Event 2) Levels: passive 5 V ƒ active 0 V ƒ Pulse: 0 V for at least 1000 ms ƒ BlueShadow Detector 40D Instructions V7647... - Page 28 ANALOG has been selected in the SETUP menu . The control voltage must be applied against AGND . Voltage range: 0-10 V The scaling can be changed by the user . AGND Reference point of the voltage at the input external λ . BlueShadow Detector 40D Instructions V7647...
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Page 29: Connecting The Pin Header
3. Pull out the depressor tool . Check whether the cables are tightly attached . Press the pin header onto Next steps the connector . Finish the installation . Bring the device into operation . BlueShadow Detector 40D Instructions V7647... -
Page 30: Analog Control
1. Insert the plug into the male Procedure connector at the rear panel . 2. Plug the other end into an electric socket . 3. Switch on the device at the power switch Fig. 12 Power switch with male connector BlueShadow Detector 40D Instructions V7647... -
Page 31: Operation
If the device is part of an HPLC system, you should also note the following: The network connection to the router is established ƒ The chromatography software has been installed by KNAUER or a ƒ com pany authorized by KNAUER . The capillaries have been connected . -
Page 32: Status Led
(idle status) . Green flashing Device is running . Yellow Firmware upload in progress . Yellow flashing Device initialization . A serious error occured . Red flashing An acknowledgeable error occured . Blue Device in standby . BlueShadow Detector 40D Instructions V7647... -
Page 33: Operating With The Keypad
2. After turning the device on, the detector displays the following, consecutive status messages: „Initialize“ - self test of electronic com- ƒ ponents „Lamp heating“ - preheating and ignition ƒ of the D2 lamp „Calibrating“ - optical calibration ƒ BlueShadow Detector 40D Instructions V7647... -
Page 34: Selecting A Wavelength
1. Position the cursor into the field for the signal output . 2. Use the vertical arrow keys to invert the absorption value . The inversion will be indicated by „-“ in front of the signal output . Fig. 14 Signal output option „-“ BlueShadow Detector 40D Instructions V7647... -
Page 35: Menu Structure
. If you want to use the numeric pad, simply press the confirmation button to activate numeric pad . A decimal point is available via the standby key in case the numeric pad is activated . BlueShadow Detector 40D Instructions V7647... -
Page 36: Menu Structure: Overview
Operation 5.4.2 Menu structure: overview Fig. 15: Menu structure: overview BlueShadow Detector 40D Instructions V7647... -
Page 37: Device Setting Parameters
10 s) to smooth the analog out- put signal . The larger the value of the selected time constant, the stronger the applied signal smoothing . Date/Time Change real time clock date and time of the detector . BlueShadow Detector 40D Instructions V7647... - Page 38 Further, the intensity of the signal channel allows you to draw conclusions about the measurement situation of the optical flow cell path . (instal- led flow cell type, solvent usage, bubble free, etc . ) . BlueShadow Detector 40D Instructions V7647...
- Page 39 Event 3 (TTL output via pin header „EV3“) — 24V (+24V via pin header „+24V valve“) Analog output Here the offset (constant baseli- setup ne shift) and the scale (in au/V) of the integrator output can be set . BlueShadow Detector 40D Instructions V7647...
- Page 40 . 23 for details on permissi- ble signal voltages, loads, etc . Display setup Adapt Brightness and Contrast of the display, as well as the brightness of the status LED via LED power . BlueShadow Detector 40D Instructions V7647...
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Page 41: Parameters In The Device-/Lamps Glp Menu
(maximum order filter absorbance) . Firmware Currently installed Firmware Ver- Version/Display sions for detector and detector firmware display . Install. Date Display of the initial installation Service Date and last service . BlueShadow Detector 40D Instructions V7647... -
Page 42: Program Menu
. 4. Change a value, using the vertical arrow keys or the confirma- tion key combined with the numeric pad . Use the confirmation button to save the settings . BlueShadow Detector 40D Instructions V7647... -
Page 43: Creating A Program
6. Edit time, wavelength and events as done be- fore (see also step 3) . 7. Press the menu key for 2 seconds to exit the submenu . The program is automatically saved . BlueShadow Detector 40D Instructions V7647... -
Page 44: Running A Program
8. To do so, navigate the cursor to the program status symbol and use the downward key to clear the status . Also, you might press the upward key to return to hold and run mode . BlueShadow Detector 40D Instructions V7647... -
Page 45: Changing A Program
(e .g ., P01) . 4. You will be asked whether you really want to delete the program . Confirm with the confirma- tion key or deny with the menu key BlueShadow Detector 40D Instructions V7647... -
Page 46: Creating And Running A Program With Preselectable Start Time
6. Before running the program, the detector will perform a self-calibration . 7. By pressing the standby button for 2 seconds the device will immediately start to recalibrate, interrupting the standby and deactivating the wake-up program . BlueShadow Detector 40D Instructions V7647... -
Page 47: Device Links
6. Move the cursor via the horizontal arrow keys to the double arrow on the lower left corner . 7. Use the vertical arrow keys to navigate to the next line or create a new line . BlueShadow Detector 40D Instructions V7647... -
Page 48: Creating A Link
. 9. Confirm the new line number via the confirma- tion key . Now, you can edit the values of the new line, as done before . BlueShadow Detector 40D Instructions V7647... -
Page 49: Running A Link
. 11. To view the progress of the currently running link navigate to the menu Device Links . Use the horizontal arrow keys to navigate to the third display within this menu . BlueShadow Detector 40D Instructions V7647... -
Page 50: Changing A Link
3. After pressing the confirmation key , you will be asked whether you really want to delete the selected link . 4. Confirm once more with the confirmation key or deny with the menu key BlueShadow Detector 40D Instructions V7647... -
Page 51: Functionality Tests
Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge . In case of a request, the technical support of KNAUER or a provider authorized by KNAUER performs this functionality test during the installation . -
Page 52: Troubleshooting
6. Replace the patch cable to the device with that no connection could be established . Has this been successful? ƒ 7. Make sure that the IP port of the device matches the port in the chro- matography software . BlueShadow Detector 40D Instructions V7647... -
Page 53: Possible Problems And Solutions
. The relationship Rinsing the Flow Cell . ƒ of the signal to the Clean the flow cell window . ƒ light path reference Exchange lamp (spectrum source) . ƒ is very low BlueShadow Detector 40D Instructions V7647... -
Page 54: System Messages
. ”Instrument remote This entry is not executable . Quit software . controlled“ ”Invalid command” Check the cable connections . Change the entry . ”Invalid Check the validity of the parameters . parameter(s)“ BlueShadow Detector 40D Instructions V7647... - Page 55 . If you try to quit a non-existent pro- gram sequence, this message appears . ”Time table is not First load the program, then start the program . loaded“ ”Time table line is Edit the program line . empty“ BlueShadow Detector 40D Instructions V7647...
- Page 56 Troubleshooting System message ”Too many lines in Check the number of program lines . program“ A maximum of 100 program lines are possible . ”Wrong Line Change the entry in the program line . number“ BlueShadow Detector 40D Instructions V7647...
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Page 57: Maintenance And Care
Customer Support . Maintenance contract The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a sepa rate maintenance contract: Opening the device or removing housing parts . -
Page 58: Cleaning And Caring For The Device
Replacing the flow cell against the detector again . 7. Screw the knurled-head screws back into the cover and tighten . plate Result The flow cell has been assembled . Next step Connect the capillaries . BlueShadow Detector 40D Instructions V7647... -
Page 59: Cleaning The Flow Cell
The flow cell has been rinsed . Next steps Check if the baseline noise has decreased . ƒ If the rinsing does not have the desired effect, disassemble the flow ƒ cell to clean the lens . BlueShadow Detector 40D Instructions V7647... -
Page 60: Cleaning The Lens Of An Analytical Flow Cell
The flow cell lens has been cleaned . Note: If the cleaning of the lens does not have the desired effect, the lens has to be replaced . Next step Mount the flow cell onto the detector . BlueShadow Detector 40D Instructions V7647... -
Page 61: Cleaning The Light Guide Of A Preparative Flow Cell
6. Assemble the flow cell . Make sure that the new seal ring does not interrupt the light path . 7. Using the allen screwdriver, tighten the threaded ring with a torque of 1 .1 Nm . Result The light guide has been cleaned . BlueShadow Detector 40D Instructions V7647... -
Page 62: Replacing The Fiber Optics
. 4. To mount new fiber optics onto the flow cell, repeat step 1-3 in reverse order . Fig. 21 Fiber optics on the detector Result The fiber optics have been replaced . BlueShadow Detector 40D Instructions V7647... -
Page 63: Transport And Storage
. Transporting the device Carefully prepare the device for transport or storage . If you want to return your device to KNAUER for repairs, enclose the Service Request Form which can be downloaded from our website . Device data For a secure transport, note the weight and dimensions of the device (see „Technical data“... -
Page 64: Disposal
160214 . 10.2 WEEE registration number KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR) . The number classifies to category 8 and 9, which, among others, comprises laboratory equipment . -
Page 65: Technical Data
11.2 Communication Maximum data rate 50 Hz (LAN) ƒ 20 Hz (Analog) ƒ 10 Hz (RS-232) ƒ Inputs Error (IN) ƒ Start (IN) ƒ Autozero ƒ 0–10 V Analog IN ƒ Outputs Events 1–3 ƒ +5 V ƒ 24 V Valve ƒ BlueShadow Detector 40D Instructions V7647... -
Page 66: General
Overvoltage category II: Energy consumers are supplied by a fixed device . Permitted pollution degree Temperature range 4-40 °C (39 .2-104 °F) Air humidity Below 90 %, non-condensing Operating height Max . 2 000 Meters above sea level BlueShadow Detector 40D Instructions V7647... -
Page 67: Chemical Compatibility Of Wetted Materials
. The following list contains information about the chemical compatibility of all wetted materials which are used in devices made by KNAUER . The data bases on a literature research on the manufacturer specifications of the materials . The wetted materials of this device are listed in the chapter “Technical data”... -
Page 68: Polyimide (Vespel®)
This amorphous perfluorinated copolymer is inert against all commonly used solvents . However, it is soluble in perfluorinated solvents like Fluo- rinert® FC-75 and FC-40, and Fomblin perfluor-polyether solvents from Ausimont . In addition, it is affected by Freon® solvents . BlueShadow Detector 40D Instructions V7647... -
Page 69: Polychlortrifluorethylene (Pctfe, Kel-F®)
Synthetic ruby is monocrystalline alumina and gets its red color by the addition of some chromium oxide . It is biocompatible and very resistant to corrosion and wear . The material is characterized by a high hardness and a high thermal conductivity . BlueShadow Detector 40D Instructions V7647... -
Page 70: Mineral Wool
Titanium has a low weight and a high hardness and stability . It stands out due to its very high chemical compatibility and biocompatibility . Titan is applied when neither stainless steel nor PEEK are usable . BlueShadow Detector 40D Instructions V7647... - Page 71 Appendix: Installation Qualification (IQ)
- Page 72 KNAUER Wissenschaftliche Geräte GmbH . Scope The customer can request the Installation Qualification . In case of a request, the Technical Support of KNAUER or a provider authorized by KNAUER performs this functionality test during the installation . The IQ is a standardized document and includes the following: ƒ...
- Page 73 Installation Qualification (IQ) Packing list, list of hardware and software Please compare the article numbers listed on the packing list to the provided products . Note deviations here: Position Device/accessory Order number Comment Initial startup Step Action Result Passed Failed Comment Identify the device .
- Page 74 Date/signature Certificate A KNAUER employee or an employee authorized by KNAUER has checked the device and performed all tests described in the IQ . The IQ form has to be signed by a person who is authorized by the customer‘s head of laboratory .
- Page 75 Latest KNAUER instructions online: www.knauer.net/library KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Fax: +49 30 8015010 Hegauer Weg 38 E-Mail: info@knauer.net 14163 Berlin Internet: www.knauer.net © KNAUER 2021...
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