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Summary of Contents for Knauer BlueShadow Detector 10D
- Page 1 BlueShadow Detector 10D User Manual V7670 HPLC...
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Page 2: Table Of Contents
Setting the Zero Point ......... KNAUER... - Page 3 Declaration of Conformity........KNAUER Detector 10D User Manual V7670A, Version 2.3...
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Page 4: Intended Use
It is used in laboratories to analyze substance mixtures. In an HPLC system, the detector serves to detect substances in liq- uids and show their concentration. In laboratories the device can be used in the following areas: Separation of chiral substances KNAUER 10D User Manual V7670, Version 2.3... -
Page 5: Features
A test cell is preassembled to the detector. Before the detector can be used, the test cell has to be replaced by a flow cell. The fiber optics detector is delivered without a test cell. KNAUER 10D User Manual V7670, Version 2.3... -
Page 6: Eluents
Even small quantities of other substances, such as additives, modifiers, or salts can influence the durability of the materials. The solvent list contains the solvents KNAUER recommends for use. If there is any doubt, contact the Technical Support of the manufacturer. - Page 7 ® Halogenated hydrocarbons, e.g. Freon Mineral and organic acids (except in buffer solutions) ® Perfluorinated solvents, e.g. Fluorinert FC-75, FC-40 ® Perfluorinated polyether, e.g. Fomblin Solvents containing particles KNAUER 10D User Manual V7670, Version 2.3...
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Page 8: Scope Of Delivery
Scope of Delivery Scope of Delivery Note Only use original parts and accessories made by KNAUER or a company authorized by KNAUER. Delivery Detector with flow cell Power Cable Power unit Accessories kit UV-Detector 10D User manual (DE/EN) Installation Qualification Document (DE/EN) transportation securing device (standard detector) 1. -
Page 9: Safety For Users
Participation during an installation of a device or a training by the company KNAUER or an authorized company. If you do not belong to this or a comparable professional group, you may not perform the work described in this user manual under any circumstances. - Page 10 Never use the system in potentially explosive atmospheres with- device prohibited? out appropriate protective equipment. For further information, contact the Technical Support of KNAUER (see See “Contacting the Technical Support” on page 13.). Take the device out of At any time, take the device completely out of operation by...
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Page 11: Definition Of Personal And Material Damage
Type of damage Category Explanations Personal damages DANGER! Lethal or very serious injuries can occur. WARNING! Serious injuries can occur. Personal and material dam- CAUTION! Moderate injuries can occur. KNAUER 10D User Manual V7670, Version 2.3... -
Page 12: Symbols And Signs
UV-light hazard, eye injuries can occur. A device or system marked with CE fulfills the product specific requirements of Euro- pean directives. This is confirmed in a Declaration of Conformity. KNAUER 10D User Manual V7670, Version 2.3... -
Page 13: Unpacking And Setup
Only the supplied power unit is to be used to connect the device to the mains supply. Replace defective power unit only with original accessories from KNAUER. Only use power units with a permission for use from your coun- try. In case of queries contact the Technical Support. -
Page 14: Space Requirements
7. Check for damage caused during transportation. In case you notice any damage, contact the Technical Support. 8. Set up the device in its location. 9. Remove protective foil and transportation securing device (standard detector). KNAUER 10D User Manual V7670, Version 2.3... -
Page 15: Connectors On The Rear Side
Plug Connector Assignments Connector Function GROUND Reference point of the voltage at the signal inputs. AUTOZERO A signal (short-circuit to GROUND) sets the measuring signal to zero. KNAUER 10D User Manual V7670, Version 2.3... -
Page 16: Connecting Cables To The Pin Header
Short-circuit hazard. Turn off the device before connecting it to the pin header. Pull the power plug. CAUTION! Electrostatic discharge can destroy the electronics! Wear protective bracelet against electrostatic dis- charge and ground. KNAUER 10D User Manual V7670, Version 2.3... -
Page 17: Analog Port
The detector is not grounded if it is connected with a 24-V power supply to a 220-V outlet. Voltages, which may influence the measuring result, can be dis- charged if the device is detected to a grounded KNAUER device, e. g., a thermostat. Tool... - Page 18 3. Insert the first cable end. 4. Connect the second cable end to the port Ground on the remote connector of a grounded 220-V KNAUER device Result The physical connection is established. KNAUER 10D User Manual V7670, Version 2.3...
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Page 19: Initial Startup
5. Push the slide into the detector. 6. Insert the knurled-head screws and screw tight. Fig. 6 Assembling the flow cell Result Flow cell is assembled. The next step is connecting the capil- laries. KNAUER 10D User Manual V7670, Version 2.3... -
Page 20: Setting The Optical Path Length Of A Preparative Flow Cell
. Put the spacer aside. 3. Insert the cover. 4. Using the wrench, tighten the threaded ring. Extending the Optical To enlarge the optical path length, put in one or both spacers. Path Length KNAUER 10D User Manual V7670, Version 2.3... -
Page 21: Connecting The Capillaries
Fig. 9 Capillary and flow cell Note PEEK fittings withstand a maximum pressure of 50 bar. Result Capillaries have been connected and the detector is ready for operation. KNAUER 10D User Manual V7670, Version 2.3... -
Page 22: Startup Of The Device
When the self-calibration has fin- ished, the status display appears. If the self-calibration fails, switch the detector off and back on. Practical Tip Regularly check the capillaries for leaks. KNAUER 10D User Manual V7670, Version 2.3... -
Page 23: Operation
There are 3 buttons on the detector that can be used for basic operation. Using the buttons, you can monitor the device and change the settings. Note To avoid falsifying measuring values, KNAUER recommends to press the Autozero button before every measurement and after exchanging eluents. Figure Function ... -
Page 24: Menu Structure
Scaling R:0.3451 Lamp Operatin hours Control Control Control Control LAN DHCP 9600 ANALOG 115200 Serial number Firmware A-OUT 1V 0.01AU A-IN set-zero A-IN GLP-lamp 1234.1h DOA1 12345678 Firmware V01.00 Fig. 13 Menu structure KNAUER 10D User Manual V7670, Version 2.3... -
Page 25: Control
Fig. 15 Display Lamp 2. Let go of left arrow key. 3. Using both arrow keys, activate or deactivate the deuterium lamp. Result When setting is finished, the status display appears. KNAUER 10D User Manual V7670, Version 2.3... -
Page 26: Adjusting The Chromatogram
Fig. 17 Display Analog Out 2. Let go of left arrow key. 3. Using both arrow keys, set a value. Result When setting is finished, the status display appears. KNAUER 10D User Manual V7670, Version 2.3... -
Page 27: Checking On The Deuterium Lamp
Fig. 19 Display Analog-In set 2. Let go of the arrow zero keys. 3. Press Autozero. Result The applied voltage is set as the zero point and is adjusted to 0 nm wavelength. KNAUER 10D User Manual V7670, Version 2.3... -
Page 28: Entering A Scaling Value
System status can be checked in the software. To be able to control the device using chromatography soft- ware, the computer must be connected to the device either with a RS-232 cable or a LAN cable. KNAUER 10D User Manual V7670, Version 2.3... -
Page 29: Local Area Network (Lan)
Fig. 22 Display control 2. Let go of left arrow key. 3. Using the arrow key, select the baud rate. Result When setting is finished, the status display appears. KNAUER 10D User Manual V7670, Version 2.3... -
Page 30: Functionality Tests
Execution The test can carried out either by KNAUER's Technical Support or by a technical service authorized by KNAUER. The OQ documentation required for executing the OQ is with costs (once) and can be ordered separately from KNAUER. -
Page 31: Maintenance And Care
Reizung der menschlichen Netzhaut durch UV- Licht. Gebündeltes UV-Licht kann an der Durch- flusszelle oder am Lichtwellenleiter austreten. Gerät ausschalten und Netzstecker ziehen! VORSICHT! Wartungsarbeiten an eingeschalteten Geräten können zu Geräteschäden führen. Netzschalter ausschalten und Netzstecker ziehen. KNAUER 10D User Manual V7670, Version 2.3... -
Page 32: Cleaning The Flow Cell
Result Flow cell is clean. Next steps Check, if the baseline noise has disappeared. If the flushing does not have the desired effect, all flow cells can be disassembled to clean the lens. KNAUER 10D User Manual V7670, Version 2.3... -
Page 33: Cleaning The Lens Of An Analytical Flow Cell
6. Using the allen screwdriver, tighten the threaded ring. Result Flow cell can be assembled. What to do when... If the cleaning of the lens does not have the desired effect, the lens has to be replaced. KNAUER 10D User Manual V7670, Version 2.3... -
Page 34: Cleaning The Light Guide Of A Preparative Flow Cell
7. Using the screwdriver, tighten the threaded ring Result Flow cell can be assembled. What to do when... If the cleaning does not have the desired effect, the light guide has to be replaced. KNAUER 10D User Manual V7670, Version 2.3... -
Page 35: Replacing The Flow Cell
Do not touch the ends of the fiber optics with your fingers, as this could falsify the measurement. Handle the fiber optics with care, avoid impacts or hard actions. Move the fiber optics carefully without using pressure or bending it. KNAUER 10D User Manual V7670, Version 2.3... -
Page 36: Removing The Fiber Optics
2. Seal the fiber optic con- nectors with caps Fig. 28 Cap fitting on the fiber optics 3. Manually unscrew the fiber optic from the detector. Fig. 29 Fitting of the fiber optics on the detector KNAUER 10D User Manual V7670, Version 2.3... -
Page 37: Technical Data
1 x ± 2.5 V scalable, 20 bit Control Digital: RS-232, LAN-DHCP, remote connector Analog: wavelength control Manual: front panel Technical parameters Lamp operating hours Display Ambient conditions Temperature range 4 – 40 °C, 39.2 – 104 °F KNAUER 10D User Manual V7670, Version 2.3... - Page 38 121 x 129 x 187 mm (Width × Height × 121 x 129 x 236 (with flow cell) Depth) Weight 1.5 kg Protection type IP 20 Height above sea level maximum 2000 meters KNAUER 10D User Manual V7670, Version 2.3...
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Page 39: Legal Information
Damage caused by negligence or improper operation of the device and damage caused by clogged capillary Packaging and transport damage In the event of device malfunctions, directly contact the manu- facturer. KNAUER Wissenschaftliche Geräte GmbH Hegauer Weg 38 14163 Berlin, Germany Phone: +49 30 809727-111 Telefax:... -
Page 40: Disposal
(January, 2001), old devices manufactured by KNAUER are marked as waste electrical and electronic equipment: 160214 WEEE registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number belongs to category 8. -
Page 41: Index
Deuterium lamp Maximum operating hours Operation Status Arrow keys Display Autozero A-OUT Handling Control Operation qualification Firmware GLP lamp Int-Time Pin header LAMP Autozero Menu structure Start-IN port Signal and reference value Port KNAUER 10D User Manual V7670, Version 2.3... - Page 42 Flushing solution Spare parts Spring strip ANALOG-IN port Error-IN/OUT port Start IN, see pin header Supply voltage, see power supply Technical Support Test cell Tests intervals Time constant Transport damage UV light Warranty Wavelength KNAUER 10D User Manual V7670, Version 2.3...
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Page 43: Declaration Of Conformity
WEEE directive 2002/96/EC (2003) on waste electrical and electronic equipment The product was tested with a typical configuration. The mark of conformity has been applied to the rear panel: Date Berlin, 2012-11-01 Alexanda Knauer (CEO and owner) - Page 44 KNAUER Wissenschaftliche Geräte GmbH © All rights reserved. Technical data are subject to change without notice. Please check our website for latest updates and changes. Translation of the original German edition of this manual, version 2.3 Last manual update: 2015-02-05 Printed in Germany on environmentally friendly paper from sustainable forests.
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