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Knauer Liquid Handler LH 8.1 Instructions Manual

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Liquid Handler LH 8.1
Instructions
HPLC
HPLC
Document no. V6774

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  • Page 1 Liquid Handler LH 8.1 Instructions HPLC HPLC Document no. V6774...
  • Page 2 For the latest version of the instructions, visit our website: www.knauer.net/library. Copyright: This document contains confidential information and may not be repro- duced without written consent of KNAUER Wissenschaftliche Geräte GmbH. © KNAUER Wissenschaftliche Geräte GmbH 2025 All rights reserved.

  • Page 3: Table Of Contents

    Symbols and signs ............12 Liquid Handler LH 8.1 Instructions, V6774...
  • Page 4 Supported sample trays ......... . 63 Liquid Handler LH 8.1 Instructions, V6774...
  • Page 5 12.5 Taking the LH 8.1 out of service ......... . 101 Liquid Handler LH 8.1 Instructions, V6774...
  • Page 6 17.4 Metals..............111 Liquid Handler LH 8.1 Instructions, V6774...

  • Page 7: General

    These operating instructions are an integral part of the device. They have to be kept in the immediate vicinity of the device and accessible to the user at all times. You can download these and other instructions from the KNAUER web- site: www.knauer.net/library Signal words Possible dangers related to the device are distinguished in personal and material damages.

  • Page 8: Legal Information

    1.4.4 Warranty seal A blue or orange warranty seal is affixed to some devices. A blue seal is used by KNAUER’s Manufacturing or Customer Support ƒ for devices to be sold. After repair, service technicians attach an orange seal onto the ƒ...

  • Page 9: Basic Safety Instructions

    Operation in potentially explosive areas without special and additional ƒ explosion protection. Contact the KNAUER Customer Support for more information. User qualification The user is qualified to handle the device if all the following points apply: He has at least a basic knowledge of liquid chromatography.

  • Page 10: Operator Responsibility

    The autosampler consists of moving parts. A sound warning indicates ƒ a planned movement, and the operator has to stay in safe range out of the movement from the arm and the syringe. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 11: Working With Solvents

    (see chapter 12 on page 100). Specific environments 2.7.1 Explosive environment Never use the system in potentially explosive atmospheres without appropriate protective equipment. For more information, contact the KNAUER Customer Support. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 12: Cooling Room

    Devices which are shipped without the completed document “Service request form and decontamination report” will not be repaired. If you would like to return a device to KNAUER, make sure to enclose the com- pleted document: www.knauer.net/servicerequest Liquid Handler LH 8.1 Instructions, V6774...

  • Page 13: Product Information

    Supports Liquid Handler 8.1, 557 mm ver- sion, 55 × 65 cm desk size, for use with Robotic Cooler Liquid Handler 8.1, 887 mm ver- A5110 Supports sion, 85 × 65 cm desk size, for use with Robotic Cooler Liquid Handler LH 8.1 Instructions, V6774...

  • Page 14: Scope Of Delivery

    Product information Scope of delivery Note: Only use spare parts and accessories made by KNAUER or by a company authorized by KNAUER. Device Liquid Handler LH 8.1 ƒ Device modules for the LH 8.1 according to customer order ƒ Power supply cable ƒ...

  • Page 15: Inside View

    Product information 3.5.2 Inside view Legend Syringe support Syringe lock Plunger lock Plunger holder adapter Syringe cradle Needle guide Fig. 2: Inside view Liquid Handler LH 8.1 Liquid Handler LH 8.1 Instructions, V6774...

  • Page 16: Rear View

    Place the terminating resistor, see Fig. 4, into one of the ModuNet™ connectors when no ModuNet™ modules are attached. Do not place the terminating resistor in the LH 8.1 when modules are attached. See chap- ter 4.1.3.2 on page 22 for more details. Fig. 4: Terminating resistor Liquid Handler LH 8.1 Instructions, V6774...

  • Page 17: Meaning Of The Leds

    (quick) Firmware update low, high, low, high, low, high (during the whole process) Starting script or analytic function high, low (slow) (buzzer on motion is set) Show alive (locate sampler) high, low (4x, quick) Liquid Handler LH 8.1 Instructions, V6774...

  • Page 18: Symbols And Signs

    The manufacturer’s serial number so that a specific medical device can be identified. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 19: Installation And Start-Up

    4. Installation and start-up First time installation Before the Liquid Handler LH 8.1 can be used, it needs to be unpacked and installed. This guide helps you with the unpacking, installation of modules and interchanging of consumables. Teaching of modules and software instructions are explained in the software help function and in the corresponding user instruction section.
  • Page 20 The device weighs more than 18 kg. When lifting, carrying or installing the device, it may fall and cause injuries. Always take the main rail out of the shipping box together with another person. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 21 Always keep the complete shipping box, carton frames, moulded foam inlays and the transport lock in case the Liquid Handler needs to be shipped to another location. Bending of instrument parts may lead to permanent damage and non-functionality. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 22: Assembly Instructions

    1. Place the instrument on a table with sufficient space. Fig. 10: Instrument placed on a table 2. Place the instrument on its side with the tower on the desk side. Fig. 11: Instrument placed on its side Liquid Handler LH 8.1 Instructions, V6774...
  • Page 23 3. Remove the moulded foam inlay. Fig. 12: Instrument placed on its side, moulded foam inlay removed 4. Remove the moulded foam inlays and from the back and the front of the tower. Fig. 13: Front/rear inlays removed Liquid Handler LH 8.1 Instructions, V6774...
  • Page 24 Sliding the inlay along the rail Fig. 15: Inlay removed 6. Mount both supports , one on each side of the X-rail, by fastening the support screws with the TX20 screwdriver. Fig. 16: Supports mounted Liquid Handler LH 8.1 Instructions, V6774...
  • Page 25 8. Move the left support to the left side of the instrument. Fig. 18: Moving left support 9. Open the tower door and remove the transport lock with the TX20 screwdriver. Fig. 19: Transport lock Liquid Handler LH 8.1 Instructions, V6774...
  • Page 26 The tower may not move beyond the supports. This can cause the sampler to fall on one side, which can damage the device.  Make sure the supports are mounted on each side of the Liquid Handler. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 27: Installing The Modules

    3. Position the module on the desired position on the LH 8.1 by adjust- ing the clamp on the X-rail of the LH 8.1. 4. Tighten the clamp’s screw. The rear part of the clamp will move for- ward closing the clamp. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 28  Then, connect active modules to the LH 8.1 or disconnect them. Note: The maximum current drawn by attached ModuNet™ modules on the bus may not exceed 2 A. Note: If no modules are connected, insert the terminating resistor into the sampler. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 29 ModuNet™ terminating resistor inserted into an injection valve Legend Injection valve LH 8.1 rear panel ModuNet connectors Terminating resistor (placed at the end of the longest string) Dual Liquid Pump Power supply over ModuNet™ Fig. 22: Example of a ModuNet™ connection scheme Liquid Handler LH 8.1 Instructions, V6774...
  • Page 30 Terminating resistor (placed at the end of the longest string) Dual Liquid Pump Robotic Cooler Power supply over ModuNet™ Fig. 23: Example of a ModuNet™ connection scheme with Robotic Cooler, injection valve and dual liquid pump Liquid Handler LH 8.1 Instructions, V6774...

  • Page 31: Ambient Conditions

    Main connection: The electrical power supply at the operation site has ƒ to be connected directly to the nearest main power connection. Grounding: The connectors for the voltage have to be grounded ƒ accordingly. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 32: Installing The Plunger Adapter, Syringe Cradle And Syringe

    4.4.1 Recommended syringes Although a variety of commercially available syringes can be used with the LH 8.1, KNAUER recommends using only the approved models, which can be chosen in the software configuration. Note: When using a different syringe, the performance of the overall system can be very different, and it is not guaranteed that technical specifications are met.

  • Page 33: Syringe Adapter Configuration

    Plunger adapter Plunger adapter type engraving Syringe cradle Fig. 24: Plunger adapter and syringe cradle 4.4.2.1 Plunger adapter types Type ID Part number Syringe Plunger/ Image category plunger button Type A 060-700766 S - small Normal Liquid Handler LH 8.1 Instructions, V6774...
  • Page 34 Plunger protector Type C 060-700812 S - small Wide Plunger protector 4.4.2.2 Syringe cradle types Type ID Part number Syringe category Image Type 01 060-700342 S - small Type 03 060-700668 M - medium Liquid Handler LH 8.1 Instructions, V6774...

  • Page 35: Replacing The Syringe Cradle And Plunger Adapter

    A510548 Trajan Syringe SGE 100F-C/T-GT-LC Type 01 Contact the KNAUER Customer Support if your syringe is not listed in the table or if you are unsure whether your syringe is supported and which combination to use. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 36 2. Use one hand to hold the cradle lock in the unlocked position by pressing the blue button downwards. This re- quires some force. Fig. 26: Removing the syringe cradle Liquid Handler LH 8.1 Instructions, V6774...
  • Page 37 Follow the steps as shown in the table below. Process Figure 1. Lower the plunger holder until the adapter is free to be removed. 2. Remove the adapter by pulling it out. Fig. 28: Removing the adapter Liquid Handler LH 8.1 Instructions, V6774...
  • Page 38 3. Release the cradle lock, and check that the cradle is fixed to the dock. 4. Move the cradle back up into the tower until it is held by the magnet. The syringe cradle has been installed. Result ƒ Liquid Handler LH 8.1 Instructions, V6774...

  • Page 39: Exchanging Or Installing A Syringe

    1. Unlock the plunger by rotating the plunger lock to the left. 2. Unlock the syringe by rotating the syringe lock counterclockwise. 3. When both plunger and syringe locks are unlocked, remove the syringe carefully and move the plunger holder down. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 40 8. When the new syringe is installed, click on the OK button. The chromatography software will now accept this position as the new null position for the syringe. The syringe has been installed. Result ƒ Liquid Handler LH 8.1 Instructions, V6774...

  • Page 41: Disconnecting The Instrument

    Be aware that the lock is spring supported and can snap back in place 7. Remove the IO, USB, LAN and / or RS-232 connectors from the back side of the instrument. Fig. 32: Disconnecting the instrument from the power supply Liquid Handler LH 8.1 Instructions, V6774...

  • Page 42: Computer Control

    Installation and start-up Computer control Note: HPLC devices from KNAUER only work with IP addresses which have been assigned by IPv4. IPv6 is not supported. This chapter describes how to set up a chromatography system in a local area network (LAN) and how a network administrator can integrate this LAN into your company network.

  • Page 43: Connecting Devices To The Lan

    2. With additional patch cables, connect all devices individually with the router. 3. Use the power supply to connect the router to the mains power system. Next steps Set the router properties (see chapter 4.6.4 on page 38). Liquid Handler LH 8.1 Instructions, V6774...

  • Page 44: Configuring The Router

    Installation and start-up 4.6.4 Configuring the router The router is preset at the factory. Information about address, user name and password is noted in the router manual: www.knauer.net/en/ Support/Library/PC-hardware Process Procedure 1. To open the router configuration, start your Internet browser and enter the IP address (does not apply for all routers).

  • Page 45: Remote Control

    Reference point of the voltage at the signal inputs RDY IN Activated by a voltage of 0 V against GND INJECT OUT Output is active for 500 ms when the injection is done. PREP OUT Output is active for 500 ms when the program starts. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 46: Connecting The Pin Header

    Connecting cables to the multi-pin connector of a switched on device cau ses a short circuit. Turn off the device before connecting cables. Pull the power plug. Electronic defect Electrostatic discharge can destroy the electronics. Wear a protective bracelet against electrostatic discharge and ground. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 47 3. Pull out the operating tool. Note: The pin header configuration has to be set individually for each type of device. For more information, refer to the machine manuals for the pin header configurations. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 48: Operation

    The fourth movement, U, is related to the plunger of the syringe. Legend Supports Wash station mount- ed on the X-rail X-rail Tower with syringe holder X-beam Syringe door Sample tray holder mounted on the X-rail Fig. 38: Basic LH 8.1 overview Liquid Handler LH 8.1 Instructions, V6774...

  • Page 49: Teaching

    2. Move the tower with syringe and place it above the injection port Fig. 40: Tower above injection port Liquid Handler LH 8.1 Instructions, V6774...
  • Page 50 Step 2: Click Teach Depth in the chromatography software. The tower drives to the taught X- and Y-positions and places the needle guide on top of the injection port. If the position is not correct, click Cancel and repeat step 1. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 51: Sample Depth

    When the sample depth button is clicked, a window opens for teaching the sample depth in steps of 0.1–5 mm. Fig. 44: Dialogue window Teach Sample Depth Liquid Handler LH 8.1 Instructions, V6774...

  • Page 52: Priming The Syringe

    In the software direct control, the pump can be purged via a button. To this end, the solvent will be transported at 15 % pump power for 30 sec- onds. If needed, an additional pump step can be used. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 53: Modules

    Press this button to move the tower to its home position. Clear button Press this button to set the taught positions to 0.0 mm. The following chapters list additional functions that are important for the various modules that are present. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 54: Syringe

    Syringe type The SYRINGE TYPE drop-down box shows a selection of predefined syringes, which can be used with this sampler. KNAUER offers gas tight syringes that are tested and proven to work well within the specified ranges. Null position Indicates the relative position of the plunger at zero volume.

  • Page 55: Injection Valve

    Use the supplied ModuNet™ cable to connect the valve to the LH 8.1. See chapter 4.1.3.2 on page 22 on how to properly connect one or more ModuNet™ modules to the LH 8.1. Legend ModuNet™ bus connectors Fig. 47: Rear view of an injection valve Liquid Handler LH 8.1 Instructions, V6774...

  • Page 56: Connections Of The Injection Valve

    InjectionValve_X (where X is the number of valves). The configuration can be done in chromatography software like ClarityChrom. For teaching of the injection valve, see chapter 5.2 on page 43. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 57: Wash Stations

    6.4.1 Configuring the wash stations Fig. 48: Wash station configuration The following functions can be accessed in the configuration tool of the chosen software package and are similar for all wash stations. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 58 For some modules (Sample Racks in container or Fast Wash Station), the sample depth has to be carefully approached in order to avoid needle damage. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 59: Fast Wash Station

    Solvent 2 rinse port Solvent 1 rinse port Waste position Fig. 49: Fast Wash Station, top view Legend Solvent 2 supply Solvent 1 supply Waste outlet Additional waste outlets (1/16”) Fig. 50: Fast Wash Station, bottom view Liquid Handler LH 8.1 Instructions, V6774...
  • Page 60 Pump 2 output Fig. 51: Dual Liquid Pump, rear view Legend ModuNet™ bus connectors Pump 2 input Pump 1 input Fig. 52: Dual Liquid Pump, front view Note: All solvent connections are fingertight 1/8“ tube fittings. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 61 Do not place the wash solvents for example in the eluent tray of your HPLC. Note: Make sure to place the bottles so that the tubings are not in the way of the LH 8.1 arm. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 62 See chapter 4.1.3.2 on page 22 on how to properly connect one or more ModuNet™ modules to the LH 8.1. Re-attach the power cable and switch on the LH 8.1. The Fast Wash Sta- tion will be automatically recognized and added to the configuration as FastWashStation_X. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 63 The Dual Liquid Pump remains powered on when the LH 8.1 is switched off. After approximately 20 seconds, it switches to safety mode. This is indicated by red LEDs. It returns to normal mode once LH 8.1 is in its booting process. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 64: Passive Wash Stations

    Wash station 5 10 ml solvent + 1 10 ml waste Legend Solvent 2 Solvent 1 Reference vial, waste Connector for waste tubing × × Fig. 60: Wash station 2 60 ml Solvent + 1 10 ml waste Liquid Handler LH 8.1 Instructions, V6774...
  • Page 65 Vials for solvent 1–4 × × Fig. 61: Wash station 4 10 ml solvent + 1 10 ml waste × × Fig. 62: Wash station 4 10 ml solvent + 1 10 ml waste integrated in the sample tray holder Liquid Handler LH 8.1 Instructions, V6774...

  • Page 66: Sample Trays

    XY direction. If the position is not correct, check whether the right sample tray is selected and if the X- and Y-positions are correctly taught. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 67 4. Press the Set button to clear the distance to reference point. The reference position field will now show the sample depth of the vial, see Fig. 66. Fig. 66: Teaching the sample depth Liquid Handler LH 8.1 Instructions, V6774...
  • Page 68 Select FrontAndRear if the sample tray covers the entire drawer. Select Front or Rear if a sample tray covers half a drawer. Note that Front is the position just behind the drawer front plate. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 69: Supported Sample Trays

    Position 1 (A1) Fig. 69: Sample tray 10 x 6 (10x, 6y) Legend Reference vial Position 12 (A12) Position 96 (H12) Position 85 (H1) Position 1 (A1) Fig. 70: Sample tray MTP96 (deep well plate 96) Liquid Handler LH 8.1 Instructions, V6774...
  • Page 70 Position 1 (A1) Fig. 71: Sample tray MTP96 (microtiter plate 96) Legend Reference vial Position 24 (A24) Position 384 (P24) Position 361 (P1) Position 1 (A1) Fig. 72: Sample tray MTP384 (microtiter plate 384) Liquid Handler LH 8.1 Instructions, V6774...

  • Page 71: Robotic Cooler

    Fig. 74: Rear view Robotic Cooler 6.6.1 Scope of delivery Note: Only use spare parts and accessories made by KNAUER or by a company authorized by KNAUER. Robotic Cooler with optional ordered sample trays ƒ Power adapter 48 V, 50–60 Hz and power cord ƒ...

  • Page 72: Installation And Start-Up

    Electronic defect Electronic hazard when using an identically constructed power adapter from another manufacturer. Only use spare parts and accessories from KNAUER or a company authorized by KNAUER. 6.6.2 Installation and start-up 6.6.2.1 First time installation Before the Robotic Cooler can be used, it needs to be unpacked and installed first.
  • Page 73 1. Remove the boxes containing the tray(s) and power supply boxes. Fig. 76: Shipping box with removed accessories 2. Open the Robotic Cooler shipping box and remove the cardboard sheet. Fig. 77: Shipping box with removed accessories and opened Robotic Cooler shipping box Liquid Handler LH 8.1 Instructions, V6774...
  • Page 74 4. Gently move the Robotic Cooler out of its shipping box by grab- bing and pulling out the two bottom side protectors. Fig. 80: Remove Robotic Cooler from shipping box 5. Remove the paddings. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 75 Always keep the complete shipping box and the protection pad- ding in case the Robotic Cooler needs to be shipped to another location. Bending of instrument parts during transport can lead to permanent damage and non-functionality. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 76 Robotic Cooler or other connected devices.  If connectors need to be plugged in the Robotic Cooler, position the device in a way that the rear side is visible and accessible.  Make sure to plug in connectors correctly. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 77 Fig. 85: Touch buttons of the Robotic Cooler 3. Power up the LH 8.1 by con- necting it to the power supply and pressing the power button on the rear side. Fig. 86: LH 8.1 power button Liquid Handler LH 8.1 Instructions, V6774...

  • Page 78: Disconnecting The Robotic Cooler

    To remove the power connector, pull the power connector sleeve backwards to release the lock. Then, gently pull the power plug out of the power connector. 4. Remove the ModuNet™ cable from the back side of the instrument. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 79: Configuring The Robotic Cooler With Sample Trays

    LED color. LED color Meaning Remarks Mainboard is booting Yellow Positions Reference run (homing) required. unknown Touch to close drawer to position 0.0. Green Positions known Drawer is opened or closed. Blue Drawer moving Liquid Handler LH 8.1 Instructions, V6774...

  • Page 80: Operating Via Chromatography Software

    Maximum fan speed The user can decide to limit the maximum fan speed to a value in the range of 20 to 100 %. By default, the maximum fan speed is set to 70 %. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 81: Main Led Indicator Color

    Note: When cooling in a humid environment, it is possible that conden- sation is formed on the trays and the drawers. Make sure to keep humidi- ty as low as possible. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 82: Cleaning And Care Of The Device

    Remove all power connectors and insert the supplied slotted screwdriver in the opening at the back of the Robotic Cooler. Find the notch in the back of the motor and turn counterclockwise to open the drawer. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 83: Obstacle

    (mm) of this bay. This allows the tower to reach a defined position inside the obstacle region (white) while avoiding the teach posi- tion with a special trajectory (black arrows). The area beyond the bay inlet is forbidden (red). Liquid Handler LH 8.1 Instructions, V6774...
  • Page 84 There is a possibility that it will hit mechanical boundaries. This is normal behavior. Note: Make sure to take the dimensions of the tower into account when defining an obstacle with a bay inlet. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 85: Injection System Principle

    The maximum injection volume equals 50 % of the loop volume. The sy- ringe takes up 1.5 times the injection volume and fills the nominal injec- tion volume into the sample loop. This injection mode should be used for preparative applications. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 86: Full Loop Filling

    The software will ensure that the right syringe size is chosen so that the sample amount can be taken up. The user has to ensure that the sample volume in the vial is sufficient to ensure reproducible results. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 87 The overfill flows into the connected waste. Wash solvent Eluent Sample Syringe injector Syringe port waste Column Pump Detector Solvent waste Washing module Sample storage module Injection module Fig. 93: Full loop: filling the sample loop Liquid Handler LH 8.1 Instructions, V6774...
  • Page 88 Wash solvent Eluent Sample Syringe injector Syringe port waste Column Pump Detector Solvent waste Washing module Sample storage module Injection module Fig. 95: Full loop: washing Liquid Handler LH 8.1 Instructions, V6774...

  • Page 89: Sandwich Injection

    If programmed, the syringe starts with pre injection wash to clean and prime the syringe. Wash solvent Eluent Sample Syringe injector Syringe port waste Column Pump Detector Solvent waste Washing module Sample storage module Injection module Fig. 97: Sandwich injection: initial Liquid Handler LH 8.1 Instructions, V6774...
  • Page 90 Wash solvent Eluent Sample Syringe Syringe injector waste port Column Pump Detector Solvent waste Washing module Sample storage module Injection module Fig. 99: Sandwich injection: building the sandwich 2 Liquid Handler LH 8.1 Instructions, V6774...
  • Page 91 Wash solvent Eluent Sample Syringe injector Syringe port waste Column Pump Detector Solvent waste Washing module Sample storage module Injection module Fig. 101: Sandwich injection: filling the sample loop Liquid Handler LH 8.1 Instructions, V6774...
  • Page 92 Wash solvent Eluent Sample Syringe injector Syringe port waste Column Pump Detector Solvent waste Washing module Sample storage module Injection module Fig. 103: Sandwich injection: washing Liquid Handler LH 8.1 Instructions, V6774...

  • Page 93: Partial Loop Filling

    The syringe is quantitatively filled with the sample by aspirating 1.5 times the sample volume. Wash solvent Eluent Sample Syringe injector Syringe port waste Column Pump Detector Solvent waste Washing module Sample storage module Injection module Fig. 105: Partial loop: taking up sample Liquid Handler LH 8.1 Instructions, V6774...
  • Page 94 To stop any back-flush of sample, the syringe remains in the injection socket during valve switching. Wash solvent Eluent Sample Syringe injector Syringe port waste Column Pump Detector Solvent waste Washing module Sample storage module Injection module Fig. 107: Partial loop: injecting Liquid Handler LH 8.1 Instructions, V6774...

  • Page 95: Overlapped Injections

    In the time after the post injection cleaning steps while the previous measurement is still running, the LH 8.1 is in ready mode waiting for the end of run from other devices. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 96 Example for a timetable without and with overlapped injections Note: The programmed time after which the next injection should be prepared has to exceed the time needed for post injection cleaning steps. Note: When set to 0.0 min, overlapped injections are disabled. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 97: Connecting Capillary And Tubing

    Due to hardware limitations, the solvent storage height cannot exceed the height of the pump. Otherwise, the solvent will be pushed out due to gravity. Note: See also chapter 6.4.2 on page 53 for a detailed installation description. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 98: Connecting The Waste

    Make sure that the waste tube is not kinked so that the liquid can drain. Additionally, the injection valve has a waste connection – port 6 (see chapter 8.1 on page 91). Liquid Handler LH 8.1 Instructions, V6774...

  • Page 99: Connecting The Lh 8.1 To Other Devices

    Controlling the LH 8.1 with chromatography software The LH 8.1 is controlled directly via software, e. g. ClarityChrom® by KNAUER. Connect the LH 8.1 to the network using the LAN connection at the rear panel of the device. 9.1.1 Checking and configuring the modules and parameters of the LH 8.1...

  • Page 100: Flushing The System

    Flushing the system Flush the system before connecting a column. Note: KNAUER recommends using a mixture of water and isopropanol (80 % : 20 %) or the mobile phase as flushing solution. The following steps apply to ClarityChrom®. 9.2.1 System flushing with ClarityChrom®...

  • Page 101: Functionality Tests

    The test intervals depend on the usage of the device. Execution The test can be carried out either by the technical support of KNAUER or by a provider authorized by KNAUER. For further information visit our website:...

  • Page 102: Troubleshooting

    6. Replace the patch cable of the device to which no connection can be established. 7. Make sure that the IP port of the device matches the port in the chro- matography software. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 103: System Messages

    Clear the error and initialize the invalidated. sampler. Robotic Cooler drawer error. Check that it is not mechanically blocked. Manual control of a Robotic Cool- Unlock the Robotic Cooler to allow er drawer was blocked. manual control. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 104 Add and configure an injector. No sampletray configured. Add and configure a sample tray. Invalid washstation solvent vial. Add and configure a wash station. No fast washstation configured. Add and configure a wash station. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 105 Repeat the teaching. invalidated. Invalid teach XY-position too close Repeat the teaching. to position zero. View Last errors and clear them. General teach error. Specifics are reported prior to this. Positions are Repeat the teaching. invalidated. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 106: Maintenance And Care

    Proper maintenance of your HPLC device will ensure successful analyses and reproducible results. 12.3 Maintenance contract Opening the device or removing housing parts may only be performed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 107: Cleaning And Care Of The Device

    2. Pack the power supply cable together with the device. 3. Disconnect remaining electrical connections and dismount all accessories. Next steps Carefully prepare the device for transport or storage (see chapter 13 on page 102). Liquid Handler LH 8.1 Instructions, V6774...

  • Page 108: Transport And Storage

    Lifting: Grab the device around the center of both sides and lift it into ƒ the packaging. 13.2 Transporting the device Documents: If you want to return your device to KNAUER for repairs, ƒ enclose the “Service request form and decontamination report“, which can be downloaded from our website.

  • Page 109: Disposal

    14.1 AVV-Marking Germany According to the German “Abfallverzeichnisverordnung” (AVV) (January, 2001), old devices manufactured by KNAUER are marked as waste electri- cal and electronic equipment: 160214. 14.2 WEEE registration number All WEEE registrations of KNAUER and the corresponding categories can be viewed on our website: www.knauer.net/imprint...

  • Page 110: Technical Data

    Display Sample injection modes Full loop, Sandwich, Partial loop Maximum back pressure 1 240 bar Sample capacity 6 sample racks per Robotic Cooler ƒ Up to 4 Robotic Coolers ƒ 2 racks for manual rack holder ƒ Liquid Handler LH 8.1 Instructions, V6774...

  • Page 111: Communication

    0.005 % with chlorhexidin and Fast Wash Station Note: Always use the 48 V power adapter supplied by KNAUER. Other power adapters can harm the LH 8.1 autosampler. The LH 8.1 autosam- pler is protected via the supplied LPS power adapter. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 112: Repeat Orders

    Sample rack 60 × 1.5 ml vials for LH 8.1 Robotic Cooler A51041 or manual rack holder Sample rack 130 × 1.5 ml vials for LH 8.1 Robotic Cooler A51042 or manual rack holder Sample rack 384 MTP (384 samples microtiter plate) A51043 Liquid Handler LH 8.1 Instructions, V6774...
  • Page 113 Accessory kit Accessory kit LH 8.1 A5107 Tools AZURA® tool kit A1033 Power cable M1651 M1278 Switzerland M1597 Europe M1642 Documents Liquid Handler LH 8.1L Instructions V6774 Installation Qualification document VIQ-Device Operation Qualification document VOQ-AS VOQ-LH Liquid Handler LH 8.1 Instructions, V6774...

  • Page 114: Chemical Compatibility Of Wetted Materials

    The following list contains information about the chemical compatibility of all wetted materials which are used in devices made by KNAUER. The data bases on a literature research on the manufacturer specifications of the materials. The wetted materials of this device are listed in the chapter “Technical data”.
  • Page 115 This amorphous perfluorinated copolymer is inert against all commonly used solvents. However, it is soluble in perfluorinated solvents like Fluor- inert® FC-75 and FC-40, and Fomblin perfluoro polyether solvents from Ausimont. In addition, it is affected by Freon® solvents. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 116: Non-Metals

    Synthetic ruby is monocrystalline alumina and gets its red color by the addition of some chromium oxide. It is biocompatible and very resistant to corrosion and wear. The material is characterized by a high hardness and a high thermal conductivity. Liquid Handler LH 8.1 Instructions, V6774...

  • Page 117: Metals

    Titanium has a low weight and a high hardness and stability. It stands out due to its very high chemical compatibility and biocompatibility. Titan is applied when neither stainless steel nor PEEK are usable. Liquid Handler LH 8.1 Instructions, V6774...
  • Page 118 Customer approval Prior to installation at the customer site, the customer has reviewed the OQ document and agrees with the design and scope. Company name: Name Function Reviewed & approved Date Signature VIQ-Installation-Qualification-Device, Version 3.1 Date: Signature: Revision:...
  • Page 119 KNAUER Wissenschaftliche Geräte GmbH. Scope The customer can request the Installation Qualification. In case of a request, the technical support of KNAUER or a provider authorized by KNAUER performs this functionality test during the installation. The IQ is a standardized document and includes the following: ƒ...
  • Page 120 Installation Qualification Tests Test Description Specification Passed Failed Comment /LOR No. Identify the device. The name on the device matches the name on the delivery order. Check the device for No transport damage is transport damage. observed. Check the scope of The scope of delivery delivery.
  • Page 121 List of rectifications (LOR) Type of Description of Persons LOR No. Test No. Action plan Due date Date/signature deviation* the deviation responsible * Type of deviation: A = acceptable (e.g. not a GMP-critical deviation) N = not acceptable Continuation of qualification activities into the next qualification phase is only possible when deviation is rectified. T = temporarily acceptable a) Release and use of the system is possible, even when the deviation is not rectified.
  • Page 122 List of changes to the document Revision no. Description of change Additional information Date/signature VIQ-Installation-Qualification-Device, Version 3.1 Date: Signature: Revision:...
  • Page 123 Installation Qualification (IQ) for a Device 10. Certificate and approval A KNAUER employee or an employee authorized by KNAUER has checked the device and performed all tests described in the IQ. The IQ form has to be signed by an authorized person. The scope of the IQ meets the customer‘s requirements.
  • Page 124 Appendix: List of supporting documents Test no. Description VIQ-Installation-Qualification-Device, Version 3.1 Date: Signature: Revision:...
  • Page 125 Latest KNAUER instructions online: www.knauer.net/library KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Fax: +49 30 8015010 Hegauer Weg 37-38 E-mail: info@knauer.net 14163 Berlin Internet: www.knauer.net © KNAUER 2025...

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