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Summary of Contents for Knauer BlueShadow 40D HPLC
- Page 1 BlueShadow Detector 40D User Manual V7646 HPLC...
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Page 2: Table Of Contents
Table of Contents Table of Contents Note For your own safety, read the manual and always observe the warnings and safety information on the device and in the manual! Intended Use ............Device Overview . - Page 3 Table of Contents Status Display Settings ........... Main Menu .
- Page 4 +49 30 8015010 Thank you. A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit contacter KNAUER par email ou par fax avec le no. de série: support@knauer.net +49 30 8015010 Merci beaucoup.
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Page 5: Intended Use
Intended Use Intended Use Note: Only use the device for applications that fall within the range of the intended use. Otherwise, the protective and safety equipment of the device could fail. Device Overview The touchscreen, the LED light for standby mode, and the flow cell can be found on the detector's front side. -
Page 6: Operating Range
Flexible options for use in the entire field of LC applications due to a comprehensive range of flow cells for the KNAUER detectors, from nano HPLC cells with flow rates » 100 nl/min to preparative flow cells with 10 l/min. -
Page 7: Eluents
Dilute sodium hydroxide (1M) Water 1.valid for the specified temperature range Scope of Delivery Note: Only use original parts and accessories made by KNAUER or a com- pany authorized by KNAUER. Delivery Detector with test cell Accessories kit User manual (DE/EN) - Page 8 At any time, take the device completely out of operation by either switching Device Securely off the power switch or by pulling the power plug. Opening the Device The device may be opened by the KNAUER Technical Support or any com- pany authorized by KNAUER only. 40D user manual V7646, version 2.0...
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Page 9: Signal Words
Symbols and Signs Signal Words Possible dangers related to the device are divided into personal and material damage in this user manual. Lethal injuries will occur. Serious or moderate injuries can occur. Minor injuries can occur. Device defects can occur. Decontamination Contamination of devices with toxic, infectious or radioactive substances poses a hazard for all persons during operation, repair, sale, and disposal of... -
Page 10: Unpacking And Setup
Only the supplied power cable is to be used to connect the device to the mains supply. Replace defective power cables only with original accessories from KNAUER. Only use power cables with a permission for use from your country. In case of queries contact the Technical Support. -
Page 11: Unpacking
Unpacking and Setup Unpacking At the factory, the device was carefully packed for safe transport. Carefully store all packaging material. Keep the included packing list for repeat orders. Tool Utility knife Procedure 1. Check for damage caused during transportation. In case you notice any damage, contact the technical support and the forwarder company. -
Page 12: Control Via Remote Connector
Unpacking and Setup Control via Remote Connector Assignment Connection Function Relay contact EV 1 The contact is on a floating basis. Its setting depends on (Event 1) the settings in the Control Unit or software. Steady-rate signal: passive = open relay contact ... -
Page 13: Analog Control
Unpacking and Setup Assignment Connection Function External Allows external analog control of the detector when the option ANALOG has been selected in the SETUP menu. The control voltage must be applied against AGND. Voltage range: 0-10 V The scaling can be changed by the user. Reference point of the voltage at the input external l ... -
Page 14: Integrator Port
Inserting the Flow Cell The supply configuration of this detector only includes a test cell (dummy cell) from KNAUER, which does not have connections for solvent. It is used, for example, to check the lamp intensity, as a dirty flow cell could distort this value. -
Page 15: Changing The Optical Path Length Of The Preparative Flow Cell
Startup Prerequisite In case necessary, the optical wavelength has been set. The device has been switched off. The power plug has been pulled. Procedure 1. Unscrew the knurled-head screws ( ) of the cover plate ( ). Hold the flow cell securely with your hand during this procedure. -
Page 16: Connecting The Capillaries
Startup 2. Remove the stainless steel cover and light guide, together with the seal ring, with tweezers. 3. To extend the path length, push the light guide out about 1 mm. Please use a clean cloth and avoid touching the light guide with your fingers. 4. -
Page 17: Configuring The Lan Settings
Startup Connecting a Device in a Local Area Net- work (LAN) to a Computer This chapter describes how to set up a local area network (LAN) and how a network administrator can integrate this LAN into a company network. The ®... -
Page 18: Configuring The Router
Startup tional patch cable is required, which is also not supplied within the scope of delivery. Legend Modules Router LAN port WAN port Workstation Fig. 8 Cabling system LAN Prerequisite Computer is on. There is a patch cable for each device and the computer. Procedure 1. -
Page 19: Switching The Detector On
Operation Note: The port ist set to 10001 at the factory. The IP port numbers in the device configuration of the chromatography software must be identical to those in the device, otherwise the connection fails. Procedure 1. Change the port number of the device. 2. -
Page 20: Handling
Operation Handling With the touchscreen it is possible to input data and commands by tapping certain areas on the screen with a finger or with a blunted object. These areas are highlighted in gray. Tapping such buttons often calls up a menu with further, self explanatory buttons. -
Page 21: Graphical User Interface Structure
Operation Button Function Explanation Confirm Month month Year Confirm the year Autozero Carry out zero balance Disable Deactivate, skip Create a new pro- gram line with time indicator Load Load program Edit Edit the program Del/Delete Deleting the pro- gram Table Program line display... -
Page 22: Status Display Settings
Operation Detector -0.01127 λ: Event Autozero Menu Program Setup Link Standby Setup Menu > Setup Menu > GLP Network Event Instrument Validation Lamps Analog Date Lamps Analog T-const. Components Intensity Fraction Calibration Fig. 11 User interface structure (2/2) Status Display Settings The following settings can be made in the status display: Signal options There are two options for signal output. -
Page 23: Program Menu
Operation The individual menus are opened by tapping the correspondingly labeled buttons. Program Menu The detector can store ten programs with 99 program lines each. Program 10 (WU) is reserved for a wake-up program for a time-delayed execution of the links or programs. The individual programs are labeled with numbers. -
Page 24: Creating Program Lines
Operation Program list Edit Edit: Program 1 Line: 1 λ: Event 0.0 min New program line - Time: 0.1 min > Time Fig. 15 Creating a Program Creating Program Lines New program lines can be created while creating a program. Tap Tab in the program's editing window to display the program lines. -
Page 25: Running A Program
Operation Running a Program 1. Tap the Program menu in order to display the program list. 2. Tap the desired program number and Load to load the program. should be tapped to stop the program. should be tapped to interrupt the program. should be tapped to stop the program. -
Page 26: Link Menu
Operation should be tapped to go to the superordinate level, or hold for two seconds to go to the Main menu. Edit: Program WakeUp 01: 05: 2010 Date: (DD:MM:YYYY) 11: 05: 00 Time: (hh:mm:ss) Program WakeUp: Fig. 18 Program > WU > Edit WU >... -
Page 27: Executing A Link
Operation should be tapped to save the settings. 7. Select the desired option for wait (diagram C). should be tapped to go to the superordinate level, or hold for two seconds to go to the Main menu. Link List Edit Edit: Link Line: 1... -
Page 28: Deleting A Link
Operation Deleting a Link 1. Tap the Link menu to display the Link list. 2. Tap the desired link number and Delete to delete the link. 3. Confirm the query. should be tapped to go to the superordinate level, or hold for two seconds to go to the Main menu. - Page 29 Operation Lamps The deuterium lamp can be switched and calibrated. Setup: Lamps D2 lamp: Halogen lamp: Procedure: Calibration Fig. 24 Setup > Lamps T-const. Here a time constant can be selected from prescribed values from 0.1 s to 10.0 s, to smooth the analog output signal. Smaller time constants can only be set using LAN control with the chromatography software.
- Page 30 Operation The external input on the back of the device enables external control of Analog in the detector through a positive analog voltage that is applied against AGND. By selecting Set to zero a voltage can be defined as the spectral zero point for the wavelength 000 nm.
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Page 31: Glp Menu
Operation Setup: Event check Start-IN: Error-IN: AutoZero-IN: Active only: Event 3 Fig. 30 Setup > Event By tapping the gray field in the active only line, the condition of the event connections can be checked. Setup: Date Date: 02.05.2010 (DD.MM.YYYY) 12:48:23 Time: (hh:mm:ss) -
Page 32: Standby
Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge. In case of a request, the Technical Support of KNAUER or from a provider authorized by KNAUER performs this functionality test during the installation. The Installation Qualification is a standardized document that comes as part of the delivery and includes the following: ... -
Page 33: Possible Problems And Rectifications
Troubleshooting Possible Problems and Rectifications Problem Solution Baseline drift Maintain constant temperature conditions during the measurement Device will not turn Inspect the power cable to be sure it is plugged into the power supply Device cannot be 1. Fasten the knurled-head screws on the flow cell calibrated to prevent incursion from interfering light or an electronics error. -
Page 34: System Messages
Troubleshooting 4. If the router is integrated into a company network, pull out the patch cable from the WAN port. Can the devices communicate with the computer, even though the router is disconnected from the company network? 5. In case you own a Control Unit, check the settings in the menu Setup >... - Page 35 Troubleshooting System message Solution Data acquisition No entries are possible. First stop acquiring mea- active surement data, afterwards you can make a new entry. Filter move error Switch the device off and on. If the system message appears again, notify the manufacturer for technical support.
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Page 36: Maintenance And Care
Proper maintenance of your HPLC device will ensure successful analyses and reproducible results. The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract: ... -
Page 37: Cleaning And Caring For The Device
Maintenance and Care Cleaning and caring for the device Device defect Intruding liquids can cause damage to the device. Place solvent bottles next to the device or in a solvent tray. Moisten the cleaning cloth only slightly. Surface All smooth surfaces of the device can be cleaned with a mild, commercially available cleaning solution, or with isopropanol. -
Page 38: Cleaning The Lens Of An Analytical Flow Cell
Maintenance and Care Cleaning the Lens of an Analytical Flow Cell Legend PTFE seal Lens Compression part Threaded ring Fig. 33 Flow cell with lens and compression part Prerequisite The device has been switched off. The flow cell has been removed. ... -
Page 39: Cleaning The Light Guide Of A Preparative Flow Cell
Maintenance and Care Cleaning the Light Guide of a Preparative Flow Cell The preparative flow cells have a rod-shaped light guide instead of the con- cave lens of the analytic cells. Legend Threaded ring Stainless steel cover PEEK spacer Seal holder (compres- sion bushing) Light guide with PTFE seal ring... -
Page 40: Replacing The Flow Cell
Maintenance and Care Replacing the Flow Cell UV light will cause the flow cells to become blind with time (solarization), making them no longer suitable for use. Legend Inlet Outlet Knurled-head screws Cover plate Slide Fig. 35 Replacing the Flow Cell ... -
Page 41: Removing The Fiber Optics
Technical Data Removing the Fiber Optics Prerequisites The device has been switched off. Auxiliary material Blind fittings for the flow cell Note: Do not touch the ends of the fiber optics with your fingers, as this could falsify the measurement. Procedure Process Figure... -
Page 42: Storage
Storage Linearity 2,0 AU at 270 nm (ASTM E1657-98) Time constants 0.1/ 0.2/ 0.5/0.1/ 1.0/ 2.0/5.0/ 10.0 s Integration time Automatic Communication Maximum data rate 50 Hz (LAN), 20 Hz (Analog), 10 Hz (RS-232) Inputs Error (IN), Start (IN), Autozero, 0–10 V Analog IN Outputs Events 1–3, +5 V, 24 V Valve Analog outputs... -
Page 43: Declaration Of Conformity
2001), old devices manufactured by KNAUER are marked as waste electrical and electronic equipment: 160214. WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number belongs to cate- gory 8 and 9, which, among others, comprise laboratory equipment. - Page 44 Abbreviations and Terminology Terminology Meaning Gradient Time-dependent composition of solvent (mobile phase) on low-pressure or high-pressure side of system. HPLC High pressure liquid chromatography (HPLC). Integration time The integration time determines how fast the detector reacts to changes in absorbance. IP address Unique address of transmitter or receiver in local network or Internet (Internet protocol).
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Page 45: Index
Index Index Abbreviations Hotline Additives Europe Ambient conditions Analog in Initial Startup Analog out Installation Analog port Integrator, see analog port Example Intended Use AVV marking Intensity IP port Buttons Capillaries Keys Capillary Touchscreen Clamping ring Keys, see buttons Fitting Torque Lamps Care... - Page 46 Index Switching the detector on Symbols Replacement System Messages Replacing the flow cell T-const. Safety Technical Data safety equipment Technical Support Salts Terminology Scan menu test Scope of Delivery Installation Qualification Setup menu Operation Qualification Simulation cell Touchscreen solvent Keys flammability transport damage line...
- Page 47 KNAUER Wissenschaftliche Geräte GmbH © All rights reserved. Technical data are subject to change without notice. Please check our website for latest updates and changes. Translation of the original German edition of this manual, version 2.0 Last manual update: 2016-04-20 Printed in Germany on environmentally friendly paper from sustaina- ble forests.
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