Daily Work With The Instrument; Specimen Freezing; Quick-Freeze Shelf; Inserting The Specimen Discs - Leica CM1510S Instruction Manual

6.

Daily work with the instrument

6.1

Specimen freezing

6.1.1 Quick-freeze shelf

Fig. 20
6.2

Inserting the specimen discs

1
Fig. 21
26
The cryochamber is equipped with a quick-freezing shelf (Fig. 20) for up
to 10 specimen discs.
When working inside the cryochamber, keep hands away from
gilled evaporator – high risk of injury from sharp edges!
Specimen freezing
• Cut the specimen to size.
The specimen block should not be any bigger than 2 x 2 cm.
• Use a specimen disc at room temperature.
• Apply a sufficient quantity of cryocompound to the disc.
• Place the specimen on the disc and orient.
• Remove the cover from the quick-freeze shelf.
• Place the specimen disc with the specimen in one of the bores of the
quick-freeze shelf and freeze the specimen.
Specimen freezing on the quick-freeze shelf can be accelerated
by additionally using a mobile or a stationary heat extractor.
• Once the specimen is frozen, insert the specimen disc in the speci-
men head and start sectioning.
Specimen discs are available in three sizes (ø 20, 25, 30 mm). The
grooved surface ensures firm contact with the specimen.
• A black dot (1) helps orienting the specimen.
Instruction manual V 1.4 – 04/2010