Fisher Scientific accuSpin 1 Operating Instructions Manual
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Fisher Scientific accuSpin 1 Operating Instructions Manual

Fisher Scientific accuSpin 1 Operating Instructions Manual

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accuSpin™ 1 / 1R
OPERATING
INSTRUCTIONS

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Summary of Contents for Fisher Scientific accuSpin 1

  • Page 1 accuSpin™ 1 / 1R OPERATING INSTRUCTIONS...
  • Page 2 How to use this manual Use this manual to get acquainted with your centri- fuge and its accessories. The manual helps you to avoid inappropriate han- Overleaf you will find a graphic dling. Make sure to keep it always close to the centri- representation of the control panel fuge.
  • Page 3 acceleration / deceleration (only for instruments with refrigeration unit) profiles run time temperature speed/RCF Quick run open lid stop start rpm/RCF Bucket selection switch key display "set" keys "set" keys Before switching on the centrifuge please read this manual...
  • Page 4 Control panel of the accuSpin™ 1 / 1R Keys start : normal start of the centrifuge Display panels stop : manual stop of a run Program selection open lid: open lid (possible only with the instrument switched on) acceleration / deceleration profiles: Quick run: short-term operation of the centrifuge as long as 1 = slow acceleration –...

  • Page 5: Table Of Contents

    Contents Contents For your safety..........3 Aerosol-tight operation ........... 27 Checking of aerosol-tight bio-containment ..28 Proper use..............3 Improper use ............3 Operation ..........29 Centrifuging hazardous materials ......3 Switching on the centrifuge ........29 Handling the centrifuge ..........4 Actuating the lid............
  • Page 6 Emergency lid release..........55 Imbalance display........... 43 Error troubleshooting..........57 Changing the settings during the run ..... 44  Contacting Fisher Scientific Service..... 63 Stopping the centrifuge .......... 44 Technical Data .......... 65 Stopping with preset run time ......44 Stopping with continuous operation ....

  • Page 7: For Your Safety

    For your safety For your safety Proper use  Fisher Scientific centrifuges are manufactured accord- The centrifuge is designed to separate liquid- ing to current technical standards and regulations. suspended materials having different densities and Nonetheless, centrifuges may pose danger to individu-...

  • Page 8: Handling The Centrifuge

    • Changes in mechanical or electrical components of protective tubes. the centrifuge may be carried out only by individuals  authorized by Fisher Scientific Handling the centrifuge • Use only original accessories for the centrifuge. The only exception are common glass or plastic centri- fuge tubes, if they are approved for the rotor speed and RCF values.

  • Page 9: Conformity To Current Standards

    For your safety Conformity to current standards Safety instructions in this manual  This symbol denotes potential hazards to Fisher Scientific centrifuges are manufactured and persons. tested according to the following standards and regula- tions: This symbol denotes potential damage to...
  • Page 10 For your safety Notes...

  • Page 11: The Accuspin™ 1 / 1R

    ™ Description of the accuSpin The accuSpin™ 1 / 1R Description The figure below shows a accuSpin™ 1R with the lid The accuSpin™ 1R is a general-purpose tabletop cen- trifuge for biotechnological and pharmaceutic research open and a swinging bucket rotor installed. that moves high capacity centrifugation onto the fast track.

  • Page 12: Safety Systems

    Description of the accuSpin ™ Safety systems Parts supplied The accuSpin™ is equipped with a number of safety Accessories supplied with the centrifuge are: systems: − power cord • Housing and lid is constructed of 8 mm steel. • Lid with window −...

  • Page 13: Function And Features

    ™ Description of the accuSpin Function and features Basic unit/ function Description / feature Cabinet / frame galvanized steel Chamber stainless steel Drive Brushless induction drive Key pad and display key pad and display elements covered by an easy-care continues surface Control Microprocessor driven by "Easycontrol II"...
  • Page 14 Description of the accuSpin ™ The Easycontrol user interface Function Feature Acceleration / deceleration profile 1 = slow acceleration – brake off 2 = fast acceleration – brake on Setting speed by rpm adjustable from 300 rpm to 15 000 rpm (depending on the rotor), - in 10 rpm increments RCF selection upon activation of RCF switch , the RCF value can then be entered...

  • Page 15: Before Use

    Before use Before use The centrifuge can be damaged by Centrifuge transport and installation jolting during the transport! After opening the box remove the protective materials. Transport the centrifuge only in the upright position using proper con- tainment and secure it properly. Han- When transporting centrifuge,...

  • Page 16: Main Connection

    Before use • The centrifuge must be protected from heat and Main connection direct sunlight. Connect the centrifuge only to an grounded main • The location must be well ventilated at all times. power supply. Make sure that the power cord is com- patible with the safety regulations valid, and that your main voltage and frequency correspond to the specifi- cations on the instrument label.

  • Page 17: Rotors And Accessories

    Rotors and accessories Rotors and accessories A rotor is not included as part of a accuSpin™ 1 or 1R centrifuge. In addition, there are adapters and reduction sleeves for a variety of commercially available tubes and bot- tles. Please consult our sales documentation for a complete collection of accessories including technical data and order numbers.

  • Page 18: Rotors For The Accuspin™ 1

    Rotors and accessories Rotors for the accuSpin™ 1 Table 1: Rotor designation Swinging Bucket Rotor 7500 3450 With bucket Round bucket Rectangular bucket Carrier for 400 ml 150 ml microplates Order no. 7500 3451 7500 3452 7500 3453 Maximum permissible load [ g ] 4 x 600 4 x 510 4 x 450...
  • Page 19 Rotors and accessories Table 1: Sealed Rotor Rotor designation 48 x 15 ml 7500 3454 Order no. Maximum permissible load [ g ] 4 x 510 Maximum speed n [ rpm ] 6000 Maximum RCF value at n 6238 Radius (max.) [ cm ] 15.5 Tube angle...

  • Page 20: Rotors For The Accuspin™ 1R

    Rotors and accessories Rotors for the accuSpin™ 1R Table 2: Rotor designation Swinging Bucket Rotor 7500 3450 With bucket Round buckets Rectangular buckets Carrier for 400 ml 150 ml microplates Order no. 7500 3451 7500 3452 7500 3453 Maximum permissible load [ g ] 4 x 600 4 x 510 4 x 450...
  • Page 21 Rotors and accessories Table 2: Sealed Rotor Micro Liter Rotor Rotor designation 48 x 15 ml 18 x 1.5 / 2.0 ml 7500 3454 7500 3455 Order no. Maximum permissible load [ g ] 4 x 510 18 x 4 Maximum speed n [ rpm ] 6000...
  • Page 22 Rotors and accessories Adapter Table 3: Adapter (1) * max. tube length with aerosol-tight cap max. tube Adapter and accessories for tubes color order no. dimensions diameter round buckets 7500 3451 x length / * [ mm ] rotor [ mm ] ®...
  • Page 23 Rotors and accessories Table 3: Adapter (2) * max. tube length with aerosol-tight cap max. tube tubes color order no. Adapter and accessories for dimensions diameter rectangular buckets 7500 3452 x length / * [ mm ] rotor [ mm ] ®...
  • Page 24 Rotors and accessories Table 3: Adapter (3) max. tube tubes color order no. Adapter and accessories for dimensions diameter sealed rotor 7500 3454 x length / [ mm ] rotor [ mm ] ® Centri-Lab Adapter type A 40 x 1.5 / 2 ml micro liter tube 11.0 x 45 black 7500 5335...

  • Page 25: Handling The Rotor

    Handling the rotor Handling the rotor Handling microplates Remove the microplate holder from carrier to load and Swinging Bucket Rotor 7500 3450 unload the microplate carrier 7500 3453. Make sure, before loading, that the rubber bottom is placed in the cut-outs of the bottom of plate carrier.

  • Page 26: Sealed Rotor 7500 3454

    Handling the rotor Sealed Rotor 7500 3454 These instructions must be read and It is essential that these instructions observed closely before each sealed are followed to prevent the centrifuge rotor is put into operation! and the rotor from being damaged and in turn causing damage to equipment and objects as well as injuries or worse still deaths.
  • Page 27 Handling the rotor Ensure all metal weights on sealed rotor are in Fit all buckets and tube racks their correct positions The sector-shaped metal weights in rotor 7500 3454 The sealed rotor must not be oper- must remain at the positions defined for these through- ated under any circumstances when out the centrifugation process to ensure stability is tube racks or buckets are being re-...
  • Page 28 Handling the rotor Filling the tubes Check the condition of the rotor lid When filling the tubes do not rely on the levels of the The life of the lid is limited to a period samples to assess weight distribution as the thickness of 5 years of the sides of the tubes and their volumes can vary.

  • Page 29: Check List For Operation With A Sealed Rotor

    Handling the rotor Insert all buckets and all tube racks. Observe the top speed of the rotor All buckets must be loaded! Load buckets evenly and symmetrically The top speeds of the sealed rotors should not be exceeded under any Do not exceed permitted weight differences: circumstances.
  • Page 30 Handling the rotor Notes...

  • Page 31: Aerosol-Tight Operation

    Handling the rotor Aerosol-tight operation Use the special lubricant 7600 3500 only to grease the seals ! Aerosol-tight rotors and tubes are only to be opened in an approved safety Spare parts are delivered with the rotor or may be or- work bench when centrifuging danger- dered separately.

  • Page 32: Checking Of Aerosol-Tight Bio-Containment

    Handling the rotor Checking of aerosol-tight bio-containment As a quick test there is the possibility to check the aerosol-tight buckets and fixed angle rotors according The checking of the rotor type and bucket was done to the following procedure: according to the dynamic microbiological test proce- dure with regard to EN 61010-2-020 appendix AA.

  • Page 33: Operation

    Operation Operation Switching on the centrifuge Locate the main power switch on the back of the centri- fuge, and set it to the ON (I) position. For a couple of Actuating the lid seconds the following reading appears in the control panel: Opening the lid Press the "open lid"...

  • Page 34: Installing The Rotor

    Operation Proceed as follows: Installing the rotor 1. Open the lid and make sure that the rotor chamber is clean. Clean any dust, foreign material or sample Improper or improperly combined residues out of chamber before use. accessories may cause severe dam- 2.
  • Page 35 Operation 4. Check whether the collet chuck is loose (collet 8. Place the rotor cover on the rotor and tighten se- chuck moves freely on the spindle). If not, loosen curely. the rotor seat using the socket wrench supplied. 5. Place the rotor on top of the collet chuck so that the Regularly check the proper positioning rotor is located precisely above the center.

  • Page 36: Loading The Rotor

    Operation Loading the rotor Maximum loading If you wish to centrifuge samples that together with the Overloading can result in destruction adapters exceed the maximum permissible load, you and severe damage to the centrifuge. must either reduce the sample volume or calculate the permissible speed n according to the following for- perm...

  • Page 37: Filling The Centrifuge Tubes

    Operation Maximum permissible load difference Filling the centrifuge tubes The smaller the imbalance of the centrifuge, the better the separation effect, because as Check carefully whether your tubes imbalance is minimized, so is the resultant are approved for the respective RCF vibration that could affect separation quality.

  • Page 38: Inserting The Centrifuge Tubes

    Operation Inserting the centrifuge tubes Fixed-angle rotors: The rotor must be loaded symmetri- cally. Failure to do so can cause rotor imbalance, which may lead to noisy operation, affect separation quality, or result in imbalance detection shut- down, as well as introduce significant detrimental wear to the motor and drive system.
  • Page 39 Operation Swinging bucket rotors: Improper loading These examples are to be applied to the other rotors in an analogous manner! Proper loading...

  • Page 40: Loading Instructions For Sealed Rotor

    Operation Loading instructions for Sealed Rotor Special loading instructions must be observed when using the Sealed Ro- tor 7500 3454! The buckets of rotor 7500 3454 should be loaded with Centri-Lab racks or inserts of identical exterior dimen- sions and weight. The rotor is intended to accommodate tubes 100 mm in length.
  • Page 41 Operation The unique construction of the Sealed 1. Attach the rotor correctly Rotor requires special precautions when operating. The following rules 2. Ensure each counter-weight is in its own must be absolutely observed in every position 1 – 4 detail. 3.
  • Page 42 Operation Notes...

  • Page 43: Entering Parameters

    Operation The value is accepted when the change back to the Entering parameters speed RCF display has occurred. Rotor / Bucket selection Deceleration curves The corresponding type of bucket The accuSpin™ offers 2 acceleration / deceleration must be selected! Otherwise the profiles for optimal centrifuging samples and gradients.

  • Page 44: Selecting Speed

    Operation 2. Push one of the "set" keys for speed. When Selecting speed doing so, the display for speed shows the actual The centrifuge speed can be set to a minimum of 300 brake cut-out speed. All other displays are blank. rpm and to a maximum of 15 000 rpm (depending on 3.

  • Page 45: Switching From Speed To Rcf Display

    Operation For faster operation, you may shift the flash- More about the RCF value ing cursor in the speed/RCF and the run The relative centrifugal force (RCF) is given in multi- time panels: just press both ples of the earth gravity g. It is a dimensionless number simultaneously.

  • Page 46: Selecting Run Time

    Operation If you keep the selected key pressed, the dis- Selecting run time play changes at first slowly and after a few You can select a run time between 1 min and 9 h 59 seconds at an accelerated pace. min and 10 h up to 99 h (from 10 h in one-hour steps), Release the key as soon as you have reached or continuous operation (hLd).

  • Page 47: Selecting The Temperature

    Operation Selecting the temperature Starting the centrifuge (accuSpin 1R only) Once the rotor is properly installed, the main switch turned on and the lid is closed, you can start the centri- You can select the temperature in the range of 0°C to fuge.

  • Page 48: Changing The Settings During The Run

    Operation in the control panel. The centrifuge starts deceleration Changing the settings during the run with the preset deceleration profile. You can change all settings during a run. By pressing As soon as the speed reaches zero, you can open the once any one of the "set"...

  • Page 49: Removing The Rotor

    Operation Removing the rotor Audible alarm 1. Open the centrifuge lid. Accompanying all error messages, a warning signal is given out which only is silenced upon pressing any key. 2. Remove the rotor cover. 3. Unscrew the clamping sleeve counterclockwise using the socket wrench supplied with the instru- Turning the centrifuge off ment until no resistance exists.
  • Page 50 Operation Notes...

  • Page 51: Maintenance And Care

    Maintenance and care Maintenance and care Cleaning Maintenance to be performed by the Pull mains plug before cleaning the customer instrument! For the protection of persons, environment and mate- rial you are obliged to clean the centrifuge regularly Clean the casing, the rotor chamber, the rotor and the and to disinfect it if necessary.

  • Page 52: Disinfection

    Maintenance and care Disinfection If a centrifuge tube containing infectious material leaks During cleaning liquids and espe- during a run, you have to disinfect the centrifuge im- cially organic solvents should not mediately. come into contact with the drive shaft and the ball bearing. Organic solvents may decompose Infectious material could enter the centri- the lubricant of the motor bearing.
  • Page 53 Maintenance and care Rotor and rotor chamber must be treated with a neu- 4. Remove the centrifuge tubes and adapters, and tral, universal disinfectant. Best suited for this purpose disinfect them or dispose of them as necessary. are disinfectant sprays, ensuring that all rotor and ac- 5.

  • Page 54: Decontamination

    Maintenance and care Decontamination For general radioactive decontamination, use a solu- Chemical additives to the steam are not tion of equal parts of 70% ethanol, 10% SDS and wa- permitted. ter. Follow this with ethanol rinses, then de-ionized water rinses, and dry with a soft absorbent cloth. Dis- pose of all washing solutions in appropriate radioactive Never exceed the maximum permis- waste containers!

  • Page 55: Sealed Rotor 7500 3454

    Maintenance and care Sealed Rotor 7500 3454 Action in the event of glass breakage Cleaning The sealed rotor can be washed and rinsed as a com- against risk of injury! plete unit. Care must be taken to ensure the central bore where the rotor rests on the chuck is dried imme- It is strongly advised that rubber diately after cleaning to prevent corrosion.

  • Page 56: Decontamination

    Maintenance and care • Removal of locked rotor Removal of swing buckets After the inside of the rotor has been rinsed with warm Removal of the unopened rotor from the centrifuge by water it should be dried. In order to do this the buckets loosening the chuck.
  • Page 57 Maintenance and care • Autoclaving • Disinfection The sealed rotor can be autoclaved for 30 minutes at If need be, the rotor can be disinfected chemically with +121°C with or without samples. a disinfectant solution instead of being autoclaved. However, only an expert who knows the type, composi- tion, character etc of the sample can decide ultimately - In principle the rotor lid should be if this action can be taken.
  • Page 58 Maintenance and care Notes...

  • Page 59: Troubleshooting

    Troubleshooting Troubleshooting During a power failure it is impossible Emergency lid release to lock the lid once the emergency lid release has been used. Never stop the In case of a power failure the lid could not be opened rotor using your hands or tools! normally using the electrical lid unlocking mechanism To permit unloading in this case, the centrifuge is equipped with an emergency override release.
  • Page 60 Troubleshooting 4. After finished, push the cord back into the instru- ment and reinserting the plastic plug. Once the power is restored, you can connect the in- strument to the main supply and turn it on. Following the self test of the centrifuge, the lid may be closed and locked with the motor.

  • Page 61: Error Troubleshooting

    Troubleshooting Error troubleshooting If problems other than those described in the following tables arise, you must contact  Fisher Scientific service. Error Behavior of the Possible cause(s) and measures to be taken centrifuge Displays remain The motor stops. Mains failure or not connected...
  • Page 62 Troubleshooting Error Behavior of the Possible cause(s) and measures to be taken centrifuge Centrifuge is exception- 1. Stop the centrifuge by pressing the "stop" key , in case of ally noisy. emergency pull mains plug. 2. Wait until the centrifuge stands still. 3.
  • Page 63 Troubleshooting Error Behavior of the Possible cause(s) and measures to be taken centrifuge Start impossible. A) Lid not properly closed Display "OPEN" appears al- Press the front of the lid firmly down. though lid is B) Overtemperature protection of the motor has been trig- closed .
  • Page 64 Troubleshooting Error Behavior of the Possible cause(s) and measures to be taken centrifuge E-00 Motor does not start. Motor or rotor blocked. 1. Switch instrument off and on again using the mains switch. 2. Open the lid. 3. Check whether the rotor can turn freely. If you cannot thus relieve the malfunction, call our Service.
  • Page 65 Troubleshooting Error Behavior of the Possible cause(s) and measures to be taken centrifuge E-06 Communication error between keyboard and main processor. Rotor stops without brak- ing to standstill. Switch the instrument off and on again. If the error persists, call Instrument cannot be our Service.
  • Page 66 Troubleshooting Error Behavior of the Possible cause(s) and measures to be taken centrifuge E-15 Check sum in NV-RAM wrong. Rotor stops without brak- ing to standstill. Instrument cannot be operated. E-17 Lid does not open. Lid blocked or jammed. Press the front part of the lid centrally down once, and press the "open lid"...

  • Page 67: Contacting Fisher Scientific  Service

    Troubleshooting Subsequently, the following readings will be dis-  Contacting Fisher Scientific Service played for 5 seconds each: Should you require our Service, please advise us of the (numbers are examples) catalog and serial number of your instrument. You will...
  • Page 68 Troubleshooting Notes...

  • Page 69: Technical Data

    26.0 kNm (19 177 ft.lb) accuSpin 1R 26.0 kNm (19 177 ft.lb) noise at maximum speed accuSpin 1 < 70 dB (A) accuSpin 1R < 64 dB (A) (sound pressure level of emission according to DIN EN ISO 11 201) Temperature set range accuSpin 1R 0°C to +40°C (32°F to 104°F)
  • Page 70 Technical Data Features specification Dimension (H x W x D) accuSpin 1 410 mm x 460 mm x 540 mm ( 16.1 x 18.1 x 21.3 inches ) 410 mm x 710 mm x 580 mm ( 16.1 x 27.9 x 22.8 inches )

  • Page 71: Electrical Connections / Fuses

    15 A 15 AT 120 V 60 Hz 8.0 A 700 W accuSpin 1 7500 3448 16 A 16 AT 230 V 50/60 Hz 4.5 A 700 W accuSpin 1 7500 3446 accuSpin 1R...

  • Page 73: Appendix

    Appendix Acceleration / deceleration profiles On the following pages you find acceleration and deceleration profiles for each rotor type respectively.
  • Page 74 acceleration profiles Swinging bucket rotor 7500 3450 4500 4000 3500 3000 2500 2000 1500 1000 time [min]...
  • Page 75 deceleration profiles Swinging bucket rotor 7500 3450 4500 4000 3500 3000 2500 2000 1500 1000 time [min]...
  • Page 76 acceleration profiles Sealed rotor 7500 3454 7000 6000 5000 4000 3000 2000 1000 time [min]...
  • Page 77 deceleration profiles Sealed rotor 7500 3454 7000 6000 5000 4000 3000 2000 1000 time [min]...
  • Page 78 acceleration profiles Micro Liter Rotor 7500 3455 16000 14000 12000 10000 8000 6000 4000 2000 0,05 0,15 0,25 0,35 0,45 time [min]...
  • Page 79 deceleration profiles Micro Liter Rotor 7500 3455 16000 14000 12000 10000 8000 6000 4000 2000 time [min]...

  • Page 81: Speed/Rcf Diagrams

    Speed/RCF diagrams = max. speed Speed/RCF diagram Swinging Bucket Rotor 7500 3450 10000 Round bucket (Rectangular bucket) = 4000 rpm = 3345 1000 Micro plate swing = 4000 rpm = 2773 1000 10000 speed (rpm)
  • Page 82 Speed/RCF diagram Sealed Rotor 7500 3454 10000 = 6000 rpm = 6238 1000 1000 10000 speed (rpm)
  • Page 83 Speed/RCF diagram Micro Liter Rotor 7500 3455 100000 = 15 000 rpm = 17 000 10000 1000 1000 10000 100000 speed (rpm)
  • Page 84 Notes...

  • Page 85: Index

    Index acceleration and deceleration curves · 67 centrifuge exceptionally noisy · 58 acceleration profiles · 7 Centrifuge transport and installation · 11 Acceleration/decelaration profile · 10 changing settings actual values · 40 during run · 44 adapter · 18 cleaning · 47 aerosol-tight operation ·...
  • Page 86 deceleration profiles · 7 E-06 · 61 decontamination · 48 E-19 · 62 Description · 7 E-22 · 62 Description / feature · 9 error codes Diagnostic messages · 10 „bAl“ · 58 disinfectant · 49 E-15 · 62 disinfection · 4 E-17 ·...
  • Page 87 symbol · 5 hLd · 42 Main connection · 1, 12 maintenance · 47 Maintenance to be performed by the customer · 47 manual lid unlocking · 55 icons manual stopping · 44 for denoting dangers and potential damage · 5 max.
  • Page 88 Plastic sample tubes approved · 30 problems run profile · 7 handling · 57 run time protective tubes range · 42 for corrosive substances · 4 selection · 42 quick run function · 44 safety instructions · 3 Quick run mode · 10 Safety instructions ·...
  • Page 89 short-time operation · 44 The printed documents consist of the delivery notes and software check this Manual · 8 internal · 29 time software version counted forward in quick run mode · 44 determination · 63 tools for installing the rotor · 30 speed of centrifugation toxins for calculation of RCF value ·...
  • Page 90 For Ordering or Technical Information In the interest of continuous product development, we reserve the right to make changes without express notice. ©2003 Fisher Scientific All Rights Reserved. Printed in Germany 20057883 accuSpin 1-1R_US Rev. 05/03...

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Accuspin 1r

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