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June 23, 2003 9:39 am, MFC_QSG_text.fm
To create a Micro Fluidic Card template or document for relative
quantification using the SDS setup file: (continued)
3. Select all wells.
4. Specify the task (Target or Endogenous Control) for each
detector.
When you import the SDS setup file, mandatory controls are
designated Endogenous Control; all other detectors are
designated Target.
SDS 2.1 software does not calculate the relative
Note:
quantity for detectors designated as endogenous controls.
If you want to designate other detectors as endogenous
controls:
a. Select all wells by clicking the top left-hand corner of
the plate view.
The entire plate is highlighted.
b. Locate the Setup tab (right-hand side of your screen).
c. In the Detector column, identify a gene
symbol/Applied Biosystems assay number that serves
as an endogenous control.
d. Select the task for the detector by clicking in the Task
column for the detector and selecting Endogenous
Control.
e. Change the task for the mandatory controls to Target.
These changes apply only to the current analysis.
Note:
Applied Biosystems Micro Fluidic Card Getting Started Guide
DRAFT
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