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Applied Biosystems Micro Fluidic Card Getting Started Guide
To create a Micro Fluidic Card template or document for relative
quantification using the SDS setup file: (continued)
5. In the Instrument tab, make sure that the default thermal
cycling conditions for Micro Fluidic Cards are set:
AmpErase
UNG
Activation
50 ° C
2 min
100% ramp
6. Save the document or continue without saving, as explained
in the following table:
If you are running...
a single Micro Fluidic Card
the first Micro Fluidic Card in
a series with identical assay
configurations
Note:
These instructions are
for running Micro Fluidic
Cards manually. For
instructions on batch
automation, refer to Chapter 6
of the user guide for the
7900HT system.
7. Enter the sample names.
Sample names serve as keys to group samples for RQ
analysis.
8. Continue with
page
26.
June 23, 2003 9:39 am, MFC_QSG_text.fm
Preparing and Running the Micro Fluidic Cards (PCR)
AmpliTaq
Gold DNA
Polymerase
Activation
94.5 ° C
10 min
100% ramp
Then...
go to step 7.
Alternatively, you can save the
document for later use.
1. Save the Micro Fluidic
2. Create a new Micro Fluidic
3. Go to step 7.
"Performing the Micro Fluidic Card Run" on
DRAFT
Each of 40 Cycles
Melt
Anneal/Extend
97 ° C
59.7 ° C
30 sec
1 min
50% ramp
100% ramp
Card document as an SDS
template document (*.sdt).
Card document (*.sds)
from the template.
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