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Thermo Scientific Accela Pump Hardware Manual

Thermo Scientific Accela Pump Hardware Manual

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Thermo
Accela Pump
Hardware Manual
60157-97000 Revision E
December 2013

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Summary of Contents for Thermo Scientific Accela Pump

  • Page 1 Thermo Accela Pump Hardware Manual 60157-97000 Revision E December 2013...
  • Page 2 © 2013 Thermo Fisher Scientific Inc. All rights reserved. Accela, ChromQuest, Foundation, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States. Super Flangeless is a trademark of Upchurch Scientific. PEEK is a trademark of Victrex PLC. The following are registered trademarks in the United States and other countries: Windows is a registered trademark of Microsoft Corporation.

  • Page 3: Regulatory Compliance

    Thermo Fisher Scientific or one of its authorized representatives. Accela Pump EMC Directive 89/336/EEC, 92/31/EEC, 93/68/EEC EMC compliance has been evaluated by TUV Rheinland of North America, Inc.

  • Page 4: Notice On The Susceptibility To Electromagnetic Transmissions

    Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument.

  • Page 5: Weee Compliance

    WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
  • Page 6 Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté...
  • Page 7 San Jose products. im Unklaren sind, setzen Sie sich, bevor Sie póngase en contacto con el personal de servicio técnico l’assistance technique pour les produits Thermo Scientific fortfahren, mit dem technischen Support für de los productos Thermo Scientific San Jose.
  • Page 8 Technical Support for Thermo Scientific im unklaren sind, setzen Sie sich, bevor Sie póngase en contacto con el personal de servicio técnico l’assistance technique pour les produits Thermo Scientific San Jose products. fortfahren, mit Ihrer lokalen technischen de los productos Thermo Scientific San Jose.
  • Page 9 す。この標識記号は機器にも表示されています。この危険の詳細につい is not included in the other categories. 危险的详细信息，参阅适当的仪器手册。若对任何步骤的安全事项有疑 ては、機器のマニュアルを参照してください。 This symbol also appears on the 问，联系 Thermo Scientific San Jose 产品的技术支持中心。 手順の安全性にご不明な点がある場合は、Thermo Scientific San Jose 製品の instrument. For details about the hazard, テクニカルサポートまでお問い合わせください。 refer to the instrument manual. When the safety of a procedure is questionable, contact Technical Support for Thermo Scientific San Jose products.
  • Page 10 作業の障害物 : 床にあるコード、ホース、その他の物体に注意してく 绊倒危险：注意地面上的线、管或其他物品。 or other objects located on the floor. ださい。 When the safety of a procedure is 手順の安全性にご不明な点がある場合は、Thermo Scientific San Jose 製品の 如对安全程序有疑问，联系 Thermo Scientific San Jose 产品的技术支持 questionable, contact Technical Support テクニカルサポートまでお問い合わせください。 中心。 for Thermo Scientific San Jose products.

  • Page 11: Table Of Contents

    Safety and Special Notices ........xvi Special Precautions for the Accela Pump......xvi Good Laboratory Practices .
  • Page 12 Pump Troubleshooting Guide........84 Accela Pump Hardware Manual...
  • Page 13 Other Parts and Assemblies ........98 Appendix A Discontinued Versions of the Accela Pump......101 Installing the Check Valves in the Discontinued LDA.

  • Page 15: Preface

    Preface This manual describes how to install, maintain, and troubleshoot the Accela Pump. You operate the Accela Pump from a computer running the Xcalibur™ data system (version 2.0 or higher) or the ChromQuest data system (version 4.2 or higher). Contents •...

  • Page 16: Safety And Special Notices

    Tip Highlights helpful information that can make a task easier. Special Precautions for the Accela Pump Note the caution sticker on the front panel of the Accela Pump shown below. The drive arm mechanism that controls the movement of the pistons contains moving parts that can pinch your fingers.

  • Page 17: Good Laboratory Practices

    Careful comparison of retention times, peak shapes, peak sensitivity, and baseline noise can provide valuable clues to identifying and solving future problems. Thermo Scientific Accela Pump Hardware Manual xvii...

  • Page 18: Sample Preparation

    LC system. However, if a leak occurs, correct it as soon as possible. Always wear eye and skin protection when operating or maintaining an LC system. Always shut down the system and return it to atmospheric pressure before attempting any maintenance. xviii Accela Pump Hardware Manual Thermo Scientific...

  • Page 19: Contacting Us

    To suggest changes to documentation or to Help • Complete a brief survey about this document by clicking the link below. Thank you in advance for your help. • Send an e-mail message to the Technical Publications Editor at techpubs-lcms@thermofisher.com. Thermo Scientific Accela Pump Hardware Manual...

  • Page 21: Introduction

    Introduction The Accela Pump is a member of the Accela™ family of ultra high performance liquid chromatography instruments. This chapter provides an introduction to the Accela Pump shown in Figure Contents • Functional Description • Specifications Figure 1. Accela Pump...

  • Page 22: Functional Description

    Note Earlier versions of the Accela Pump do not have the dynamic mixer. If you have an older version of the Accela Pump, a Thermo Fisher Scientific field service representative can install the dynamic mixer on site for a service charge and the cost of the Accela Pump Dynamic Mixer Upgrade Kit.

  • Page 23: Vacuum Degasser Assembly

    The proportioning valves of the Accela Pump incorporate a finely machined ball and seat design similar to a check valve. This ball and seat design controls the flow of solvents to a...

  • Page 24: Purge Valve

    LDA to the mixer and the mixer to the autosampler. Figure 3. View of the purge valve Connection to Purge valve autosampler Dynamic mixer PU RGE PULSE DAMPENER FLUSH Accela Pump Hardware Manual Thermo Scientific...

  • Page 25: Pulse Dampening Assembly

    LC system. The priming solvent in the loop does not interfere with the purity of the mobile phase, and its composition does not need to match the mobile phase. Thermo Scientific Accela Pump Hardware Manual...

  • Page 26: Dynamic Mixer

    If you have an older version of the Accela Pump, a Thermo Fisher Scientific field service representative can install the dynamic mixer on site for a service charge and the cost of the Accela Pump Dynamic Mixer Upgrade Kit.

  • Page 27: Status Leds

    Figure Table 1 describes these LEDs. Figure 5. Front panel LEDs Power Communication Degas Table 1. Status descriptions for the Accela Pump LEDs Status Meaning Power Green The pump is on and is receiving power. Communication Amber The pump is not communicating with the data system PC.

  • Page 28: Specifications

    100/120 V ac or 220/240 V ac, 50 to 60 Hz, 500 VA max Operating temp: + 10 °C to + 40 °C Storage temp: - 40 °C to + 70 °C Operating humidity: 5 to 95% relative, non-condensing Accela Pump Hardware Manual Thermo Scientific...

  • Page 29: Installation

    Installation This chapter provides instructions for the initial installation of your Accela Pump, including connections to other modules in the system. Use the Installation Checklist on the back of this page as a quick reference. Make a copy of the checklist and fill it out when you have successfully completed the installation.

  • Page 30: Installation Checklist

    Installation Installation Checklist Installation Checklist For the proper installation of your Accela Pump, complete these steps and keep this checklist in your maintenance records. Unpack and inspect your instrument (see “Unpacking and Inspecting the Instrument”  page 11). Read the safety precautions.

  • Page 31: Unpacking And Inspecting The Instrument

    The shipping container contains the pump, a power cable appropriate for the shipping destination, and an accessory kit. Table 2 lists the items in the Accela Pump Accessory Kit. Table 2. Accela Pump Accessory Kit Description Part number...

  • Page 32: Making The Initial Instrument Preparations

    Note Earlier versions of the Accela Pump do not have a digital I/O port. In addition, the fan vents on earlier versions of the Accela Pump are located on the bottom of the pump, instead of the back panel.

  • Page 33: Connecting The Power Cable

    Installation Making the Initial Back Panel Connections Figure 6. Back panel of the Accela Pump Input and output signal pins USB port This device complies with FCC Rules, Part 15. CAUTION 355 River Oaks Parkway Operation is subject to the following two conditions: San Jose, CA 95134 U.S.A.

  • Page 34: Connecting The Usb Cable

    Installation Making the Initial Back Panel Connections Connecting the USB Cable The Accela Pump Accessory Kit contains a USB cable that is approximately 3 m long.  To connect the pump to the computer with the USB cable 1. Connect the series A plug of the USB cable to an available USB port on the computer.

  • Page 35: Connecting The System Interconnect Cable

    IMPORTANT You can use the older 5-connector version of the system interconnect cable with Accela LC system. If you use the cable that has 5-combicon connectors, attach the connector that has a blue, LC PUMP label to the Accela Pump. Thermo Scientific...
  • Page 36 Making the Initial Back Panel Connections Figure 9. Schematic of the connections for the system interconnect cable Accela PDA Detector System interconnect cable Accela Autosampler Accela Pump RS232 1 2 3 4 5 6 7 8 Accela Pump Accela Pump Hardware Manual Thermo Scientific...

  • Page 37: Connecting The Low-Pressure Solvent Lines

    Four low-pressure solvent lines connect the solvent bottles in the solvent platform to the pump. Connect these solvent lines before you operate the pump. Because the Accela Pump contains very small, precision-machined parts that can be affected by small particles, solvents should always be HPLC-quality and free of particulate matter.

  • Page 38: Assembling The Solvent Reservoir Bottles

    5. Position the bottle in the Accela solvent platform, allowing the solvent inlet line to hang down along the left side of the Accela system. Accela Pump Hardware Manual Thermo Scientific...

  • Page 39: Installing The Super Flangeless Fittings

    Figure 11. Super Flangeless fitting components Compression ring and ferrule Tubing (with square-cut end) Figure 12. Drawing of the compression ring sides Angled side Flattened side Thermo Scientific Accela Pump Hardware Manual...
  • Page 40 Hold the tubing to the bottom of the tool while tightening down the nut. Figure 14. Using a compression tool to swage the fitting onto the tubing Compression tool (internal union and Super Flangeless nut assembly) Accela Pump Hardware Manual Thermo Scientific...
  • Page 41 6. Pass the solvent inlet line through the openings along the left side of each Accela module to complete the installation. 7. Repeat the above steps for the B, C, and D solvent inlet lines. Figure 16. Vacuum degas connections Vacuum degasser inlets and outlets Thermo Scientific Accela Pump Hardware Manual...

  • Page 42: Connecting The Pump To The Autosampler

    Pump to the heat exchanger of the Accela Autosampler. If you have an older version of the Accela Pump that does not have an integrated mixer, you can order the Accela Pump Dynamic Mixer Upgrade Kit to upgrade the pump, or you can use the precut, 20 cm length of high-pressure tubing to connect the outlet port of the inline filter holder directly to the heat exchanger of the Accela Autosampler.
  • Page 43 Figure 18. Connection between the pump and the autosampler Connection to inlet of heat LDA outlet exchanger 10 cm length of high-pressure tubing 20 cm length of high-pressure tubing Mixer outlet PU RGE Inline filter holder PULSE DAMPENER FLUSH Thermo Scientific Accela Pump Hardware Manual...

  • Page 44: Powering On For The First Time

    “Pump Troubleshooting Guide” page 84. Call your Thermo Fisher Scientific service representative if you require assistance. Before you can operate the Accela Pump, first add it to the data system configuration for your instrument (see Chapter “Data System Instrument Configuration”...

  • Page 45: Data System Instrument Configuration

    Data System Instrument Configuration To add the Accela Pump to the data system instrument configuration and to ensure that the Accela Autosampler recognizes the input signals issued by the pump, follow the procedures in this chapter. Contents • Adding the Pump to the Xcalibur Instrument Configuration •...
  • Page 46 Data System Instrument Configuration Adding the Pump to the Xcalibur Instrument Configuration Figure 19. Instrument Configuration window (for Xcalibur versions lower than 2.1) Accela Pump Hardware Manual Thermo Scientific...
  • Page 47 The installed instruments appear as buttons in the Available Devices pane. 3. In the Available Devices pane, double-click the Accela Pump button. A copy of the Accela Pump button appears in the Configured Devices pane. 4. In the Configured Devices pane, double-click the Accela Pump button.

  • Page 48: Configuring The Signal Polarity For The Accela As In Xcalibur

    Figure 22). If you do not select these two check boxes, the autosampler does not recognize the input signals from the Accela Pump, and it does not begin an injection sequence when you start a sequence. After you submit a run, the Accela Autosampler waits for a Pump Ready signal from the pump.
  • Page 49 3. Click the Signal polarity tab. The Signal Polarity page appears (see Figure 22). 4. Make sure that the Pump ready active high check box and the Injection hold release active high check box are selected. Thermo Scientific Accela Pump Hardware Manual...

  • Page 50: Configuring An Accela Instrument In Chromquest

    Right-click The Enterprise button in the left pane of the Main Menu window. b. From the shortcut menu, choose New > Instrument. A New Instrument button appears in the right pane of the Main Menu window. Accela Pump Hardware Manual Thermo Scientific...
  • Page 51 • To modify the configuration of an Accela system, do not select an instrument type. • To create a new instrument, select Accela. A message appears warning you that this selection erases the current configuration (see Figure 24). Click OK. Figure 24. Warning message Thermo Scientific Accela Pump Hardware Manual...
  • Page 52 Figure 25. Accela dialog box 5. In the Available modules pane, do the following: a. Double-click the Accela Pump button. A copy of the button appears in the Configured modules pane. b. Double-click the Accela AS button. A copy of the button appears in the Configured modules pane.
  • Page 53 Figure 27). Figure 27. Accela Pump Configuration dialog box (ChromQuest) Note In ChromQuest 5.0, the Accela Pump Configuration dialog box includes a Handle Degasser Feedback check box. This feature is reserved for future use. Thermo Scientific Accela Pump Hardware Manual...
  • Page 54 In the Default pressure units list, select the pressure units that you prefer: bar, PSI, or MPa. c. Click OK to accept the entries and close the Accela Pump Configuration dialog box. 7. Configure the Accela Autosampler: a. In the Configured modules pane, double-click the Accela AS button.
  • Page 55 In the Detector name box, type an identifying name for the PDA detector. c. In the Detector type list, select PDA. In the Acquisition source list, ChromQuest automatically selects PDA Plus ACQ. d. Click to open the PDA Plus dialog box. Thermo Scientific Accela Pump Hardware Manual...
  • Page 56 Click OK to close the Detector Configuration dialog box. 9. Click OK to close the Accela dialog box. 10. For ChromQuest SI, click Close in the ChromQuest SI Configuration dialog box to return to the computer desktop. Accela Pump Hardware Manual Thermo Scientific...

  • Page 57: Preparing The Pump For Operation

    Before you operate the Accela Pump for the first time, remove air from the solvent lines and prime the pulse dampener.

  • Page 58: Using The Data System To Start And Stop The Solvent Flow

    You can start and stop the solvent flow by using the data system start and stop controls: • Using Xcalibur to Start and Stop the Solvent Flow • Using ChromQuest to Start and Stop the Solvent Flow Accela Pump Hardware Manual Thermo Scientific...

  • Page 59: Using Xcalibur To Start And Stop The Solvent Flow

    2. In the view bar, click the Accela Pump button. The Instrument Setup view for the Accela Pump opens. 3. Choose Accela Pump > Direct Control to open the Direct Control dialog box shown in Figure Figure 30. Xcalibur Direct Control dialog box for the Accela Pump 4.

  • Page 60: Using Chromquest To Start And Stop The Solvent Flow

    The Instrument Status window appears with the Accela Pump page displayed (see Figure 31). Use the Accela Pump page to view the pump status or to start an isocratic solvent flow. Figure 31. Accela Pump page of the Instrument Status window 3.

  • Page 61: Priming The Pulse Dampener

    7. Close the pulse dampener flush valve by turning the valve clockwise. Unless you need to refill the dampener loop of the pulse dampener with fresh solvent, keep the pulse dampener flush valve closed. Thermo Scientific Accela Pump Hardware Manual...

  • Page 63: Diagnostics

    The pump pulsation should be less than 1% at backpressures greater than 100 bar (1450 psi). Note Because the Accela Pump does not use electronic pulse damping, the Accela Pump pressure fluctuation often appears to be higher than that for conventional pumps.

  • Page 64: Using Xcalibur To Monitor The Pump Status

    2. To display the Status page, click the Status tab. 3. To display the status pages for the Accela Pump, click the Accela Pump listing in the directory tree. Check the value for the standard deviation of the pressure listed on the General page (see Figure 32).

  • Page 65: Using Chromquest To Monitor The Pump Status

    Error A generic run-time error has occurred.  To check the status of the Accela Pump from the ChromQuest data system 1. Open the online Instrument window. 2. Choose Control > Instrument Status. The Instrument Status window appears with the Accela Pump page displayed...

  • Page 66: Testing The Pump Proportioning

    Diagnostics Testing the Pump Proportioning Figure 33. Pump Status area of the Accela Pump page (ChromQuest) Pump status System backpressure and pressure standard deviation 3. Check the status of the Accela Pump. 4. Check the system backpressure and the pressure SD (based on pressure readings taken over a 30 second period).
  • Page 67 100.0 60.0 100.0 The gradient program described in Table 4 should create a gradient profile containing five steps of equal height as shown in Figure 34. Inaccuracies in the step heights indicate proportioning errors. Thermo Scientific Accela Pump Hardware Manual...
  • Page 68 “Cleaning the LDA Components without Disassembling the LDA” page 56. If this fails to restore proper pump performance, disassemble and clean the LDA and replace the primary seals as described “Replacing the Primary Pistons Seals” page Accela Pump Hardware Manual Thermo Scientific...

  • Page 69: Determining The Delay Volume Of The Pump

    The gradient delay volume of the Accela Pump consists of the wetted components that lie in the flow path between the solvent proportioning valves of the pump and the outlet of the LDA.
  • Page 70 Determining the Delay Volume of the Pump 4. From the Xcalibur data system, do the following: a. In the Direct Control dialog box for the Accela Pump, select the Take the pump under control check box (see “Using Xcalibur to Start and Stop the Solvent Flow”...
  • Page 71 Determining the Delay Volume of the Pump Figure 35. Graphical display of the baseline shift that occurs as the spiked mobile phase reaches the detector Solvent proportioning valve to detector Baseline shift Inject Time (min) Thermo Scientific Accela Pump Hardware Manual...

  • Page 73: Maintenance

    • Replacing the Primary Pistons Seals • Assembling the Liquid Displacement Assembly • Connecting the LDA to the Pump • Servicing the Proportioning Valves • Replacing the Solvent Reservoir Filters • Replacing a Fuse Thermo Scientific Accela Pump Hardware Manual...

  • Page 74: Flushing The Piston Guide Bushings

    1. If the outer piece of PTFE tubing has not been cut, cut it through the middle. 2. Connect a syringe filled with distilled water to the bottom piece of outer tubing and gently flush the water through the system. Figure 36. Flushing the piston guide bushings Accela Pump Hardware Manual Thermo Scientific...

  • Page 75: Minimizing Pump Pulsation

    Air trapped in the piston chambers causes an excessive variation in the backpressure. Note All low-pressure HPLC pumps require eluents that have been properly degassed. The Accela Pump has a built-in vacuum degasser. Plumbing the pump to bypass this degasser introduces a potentially unacceptable level of air into the piston chambers.

  • Page 76: Cleaning The Lda Components Without Disassembling The Lda

    2. Set up the system to pump the same solvent on all four channels by connecting all four solvent reservoir lines to one solvent bottle. 3. Attach a 10 mL disposable syringe to the tubing attached to the purge valve. The syringe is supplied in the Accela Pump Accessory Kit (see “Accela Pump Accessory Kit ” page 97).
  • Page 77 Water 6. Close the purge valve. 7. Retest the pressure variation by returning to the initial conditions that displayed the problem. Make sure that the backpressure at these conditions is at least 40 bar. Thermo Scientific Accela Pump Hardware Manual...

  • Page 78: Cleaning The Lda Components After Disassembling The Lda

    CAUTION Do not use an ultrasonic bath to clean the check valves. Sonicating the check valves can damage their small ruby balls. Cleaning the Pistons If precipitate is visible on the pistons, clean them with a soft tissue and cleaning paste. Then rinse the pistons with methanol. Accela Pump Hardware Manual Thermo Scientific...

  • Page 79: Cleaning The Displacement Chambers And The Inlet Module

    4. After cleaning the displacement chambers and the inlet module, reinstall the PEEK film seals if they do not show signs of damage. 5. Replace the primary and secondary piston seals before assembling the LDA. Thermo Scientific Accela Pump Hardware Manual...

  • Page 80: Removing The Lda From The Pump

    LDA (see Figure 39). Note There are two versions of the LDA for the Accela Pump. In the original version of the LDA, the lower displacement chamber had an etched flow-direction arrow. Figure 39 shows the current version of the LDA.
  • Page 81 The damage might not be apparent until you attempt to run the pump. Figure 40. View of the actuating coils with their nylon screws Nylon screw White, insulated, electrical wire Thermo Scientific Accela Pump Hardware Manual...
  • Page 82 8. Using a 4 mm L-hex wrench, turn the two clamping screws 3 to 4 turns to loosen the piston spring clamps (see Figure 42). Figure 42. Loosening the clamping screws 9. Remove the two piston spring clamps as shown Figure Figure 43. Removing the piston spring clamps (retaining clips) Accela Pump Hardware Manual Thermo Scientific...
  • Page 83 Pinching a connecting wire can irreversibly damage the thin electrical wire housed within the white insulated coating. The damage might not be apparent until you attempt to run the pump. Figure 45. Pulling the LDA off the mounting pins Mounting pins Thermo Scientific Accela Pump Hardware Manual...

  • Page 84: Disassembling The Lda

    You can detach the holding hooks completely with approximately nine turns on the screw fasteners. Figure 46. Loosening the fastening screws that secure the holding hooks Fastening screws Accela Pump Hardware Manual Thermo Scientific...
  • Page 85 If you are replacing the primary piston seals but do not need to clean the stacked components of the LDA, go to “Replacing the Primary Pistons Seals” page 67. You can remove the primary piston seals from the displacement chambers without removing the displacement chambers from the LDA body. Thermo Scientific Accela Pump Hardware Manual...

  • Page 86: Removing The Stacked Components From The Lda Body

    Figure 49 shows the stacked components of the current LDA. For information on the previous version of the LDA, see Appendix A, “Discontinued Versions of the Accela Pump.” Accela Pump Hardware Manual Thermo Scientific...

  • Page 87: Replacing The Primary Pistons Seals

    Carefully insert a piston into the hole in the center of the seal, but do not push the piston further into the bore of the displacement chamber. b. Gently pry the seal out of the bore. Thermo Scientific Accela Pump Hardware Manual...
  • Page 88 Secondary piston seal Piston Holding hook 6. Slide the guide bushing onto the piston (see Figure 51). Figure 51. View of the holding hook, piston, secondary piston seal, and guide bushing assembly Guide bushing Accela Pump Hardware Manual Thermo Scientific...
  • Page 89 • If you did not remove the stacked components from the LDA, go to “Installing the Holding Hooks and the Piston Assemblies” page • If you removed the stacked components from the LDA, go to “Inserting the Components of the LDA Stack into the LDA Body” page Thermo Scientific Accela Pump Hardware Manual...

  • Page 90: Assembling The Liquid Displacement Assembly

    2. Slide the top rim of the quaternary inlet module into the bracket on the bottom of the LDA body (see Figure 54). Figure 54. Connecting the outlet module to the LDA body Lower bracket Quaternary inlet module LDA body Accela Pump Hardware Manual Thermo Scientific...

  • Page 91: Inserting The Check Valves Into The Lower Displacement Chamber

    Assembling the Liquid Displacement Assembly Inserting the Check Valves into the Lower Displacement Chamber Note There are two versions of the LDA. For information on the discontinued version, Appendix A, “Discontinued Versions of the Accela Pump.”  To install the check valves 1.
  • Page 92 Lower displacement chamber with check valves 4. Slide the compression lid into the upper bracket of the LDA body (see Figure 57). Figure 57. Sliding the compression lid into the LDA body Compression lid Accela Pump Hardware Manual Thermo Scientific...

  • Page 93: Installing The Holding Hooks And The Piston Assemblies

    To install the holding hooks and piston assemblies 1. Slide each holding hook and piston unit into its designated slot (see Figure 59). Figure 59. Sliding the holding hook and piston assembly into the LDA body Thermo Scientific Accela Pump Hardware Manual...
  • Page 94 Figure 60. View of the piston pushed into the displacement chamber 3. Using a 4 mm L-hex wrench, tighten the fastening screws (see Figure 61). Figure 61. Tightening the fastening screws Fastening screws Accela Pump Hardware Manual Thermo Scientific...

  • Page 95: Connecting The Lda To The Pump

    CAUTION Although the damage is not visible, pinching one of these connecting wires can irreversibly damage the internal copper wire, which conducts electricity to magnetize the coil. This damage causes the proportioning valve to stop functioning. Thermo Scientific Accela Pump Hardware Manual...
  • Page 96 Figure 64. Pushing the piston heads towards the drive mechanism arms Bracket Piston Drive arm head mechanism 4. Insert the two piston retaining clips between the piston heads and the brackets of the drive mechanism arms. Accela Pump Hardware Manual Thermo Scientific...

  • Page 97: Servicing The Proportioning Valves

    “Diagnostics.” Servicing the Proportioning Valves Each proportioning valve of the Accela Pump contains an electromechanically actuated ball and seat valve. The ruby ball portion of the valve sits on one end of a magnetic piston that floats within the body of the valve. The sapphire seat where the ball rests is located on the...
  • Page 98 Push the tool (which is a piece of stainless steel wire) into the valve until you contact the piston, and then gently push the piston away from the valve face. Figure 66. Proportioning valve, shown with the valve tool Valve tool Accela Pump Hardware Manual Thermo Scientific...

  • Page 99: Replacing The Solvent Reservoir Filters

    10 micron pore size stainless steel filter (P/N A4929-010 Figure 67 shows the Teflon filter. Figure 67. Solvent reservoir filter and solvent line End of solvent line that has been scored by the solvent reservoir filter Solvent reservoir filter Thermo Scientific Accela Pump Hardware Manual...

  • Page 100: Replacing A Fuse

    If the replacement fuse fails in a short time, you have a serious electrical problem. Leave the blown fuse in place and contact your Thermo Fisher Scientific service representative. Figure 68. Power entry module Release Fuses Fuse holder Accela Pump Hardware Manual Thermo Scientific...

  • Page 101: Troubleshooting

    Troubleshooting If properly maintained, the Accela Pump can operate free of trouble for many years. You can avoid most pump problems with periodic maintenance, as described in Chapter 6, “Maintenance.” However, in the event that an error message is displayed or you suspect a mechanical or electrical failure, you might be able to diagnose the source of the problem.

  • Page 102: General System Troubleshooting

    2. Refer to the troubleshooting guide for your autosampler. 3. Check sample retention with chromatographic conditions. 4. See problem 8 on page  Contaminating/ghost peaks 1. Clean the system and the column. 2. Refer to the troubleshooting guide for your autosampler. Accela Pump Hardware Manual Thermo Scientific...
  • Page 103 2. Refer to the troubleshooting guide for your autosampler.  No instrument or device control 1. Check the cable connections. 2. Check the system configuration. 3. Refer to the troubleshooting guide for each instrument. Thermo Scientific Accela Pump Hardware Manual...

  • Page 104: Pump Troubleshooting Guide

    Contact Thermo Fisher Scientific service drive circuit representative 5. Pump does not a. Incorrect connection of a. Reconnect synchronization cable (see start or stop synchronization cable “Connecting the System Interconnect Cable” remotely page Accela Pump Hardware Manual Thermo Scientific...
  • Page 105 Excessive air in the solvent lines b. Remove the air from the solvent lines (see “Removing Air from the Solvent Lines” page c. Open venting valve c. Close the venting valve on the left side of the Thermo Scientific Accela Pump Hardware Manual...
  • Page 106 1. Using a #2 Phillips screwdriver, remove the screw that secures the LED cover to the inner-left door, and then remove the cover. Screw 2. Reconnect the LED cable to the board. LED cable 3. Secure the LED cover to the door. Accela Pump Hardware Manual Thermo Scientific...

  • Page 107: Pump Pulsation

    The pump continuously monitors the system pressure and bases its pressure readout on a 30 second interval. Under normal operating conditions, the Accela Pump shows very small variation in pressure. At pressures greater than 100 bar (10 MPa, 1450 psi), the pump pulsation should be less than 1 percent.

  • Page 108: Baseline Noise At Low Pressures

    UHPLC pumps are optimized to deliver a constant flow with a minimum of pressure fluctuations when they are set up to operate at an appropriate backpressure. The Accela Pump provides stable flow in both operating modes: low pressure and high pressure. When your LC system is set up to operate at a backpressure below 7000 psi (483 bar), make sure that you select the low-pressure operating mode (0 to ~7000 psi) for your instrument control method.

  • Page 109: Insufficient Solvent Flow From The Pump Outlet

    3. Repeat steps 1 and 2 for each channel. If one or more of the values are low, clean the LDA by flushing it the appropriate solvents, as described in “Cleaning the LDA Components without Disassembling the LDA” page Thermo Scientific Accela Pump Hardware Manual...

  • Page 110: Blocked Check Valve

    Contamination of the proportioning valves is likely to cause inaccuracy in the steps. Clean the LDA by flushing it with the appropriate solvents, as described in “Cleaning the LDA Components without Disassembling the LDA” page Accela Pump Hardware Manual Thermo Scientific...

  • Page 111: Chapter 8 Accessories And Replacement Parts

    This chapter contains a list of replaceable parts and their corresponding part numbers. To place an order, contact your local Thermo Fisher Scientific service representative. Contents • Liquid Displacement Assembly (LDA) Parts • Accela Pump Accessory Kit • Accela System Kit • Solvent Interconnect Kit •...

  • Page 112: Liquid Displacement Assembly (Lda) Parts

    Check valve (inlet) 00950-01-00273 Ti/ruby/sapphire Inlet holder, check valve cartridge, and PEEK seal for the Accela Pump Cartridge, blind, inlet, Accela Pump 00950-01-00120 PEEK Lid, compression w/set screw, Accela Pump 00950-01-00125 Piston guide bushing 00950-01-00127 Ti/zirconia Quaternary inlet module 00950-01-00119 Piston, sapphire...
  • Page 113 (4) Primary piston seal (Fluoroloy 907) (2) PEEK film seal (5) Outlet holder and check valve (6) Lower displacement chamber (4) Primary piston seal (Fluoroloy 907) (7) Inlet holder and check valve (8) Inlet blind cartridge Thermo Scientific Accela Pump Hardware Manual...
  • Page 114 (4) Primary piston seal (Fluoroloy 907) (2) PEEK film seal (5) Check valve and PEEK film seals (6) Lower displacement chamber (4) Primary piston seal (Fluoroloy 907) (7) Check valve and PEEK film seals (8) Inlet blind cartridge Accela Pump Hardware Manual Thermo Scientific...
  • Page 115 (9) Compression lid LDA body (10) Guide bushing Holding hook (11) Quaternary inlet module (12) Piston (sapphire) (14) Secondary piston seal (15) Actuating coil (13) Nylon set screw (17) Proportioning valve (16) Proportioning valve seal ring Thermo Scientific Accela Pump Hardware Manual...
  • Page 116 Figure 72. View of assembled LDA and additional replaceable parts (18) Pressure sensor and pulse dampener unit (19) Wing screw for purge valve (20) Purge valve spindle (21) Crossover tube (23) Piston spring clips (22) Z-arm screw set Accela Pump Hardware Manual Thermo Scientific...

  • Page 117: Accela Pump Accessory Kit

    Accela Pump Accessory Kit The following items are contained in the Accela Pump Accessory Kit (P/N 60157-62001). Cable, assembly, RS232, shielded, external PC ......72011-63008 Cable, USB, 3 m length, ROHS .

  • Page 118: Solvent Interconnect Kit

    Kit, maintenance, Accela Pump........60157-62002...
  • Page 119 Tubing, no-ox (sold by the foot) ......00028-01-00033 Thermo Scientific Accela Pump Hardware Manual...

  • Page 121: Appendix A Discontinued Versions Of The Accela Pump

    Installing the Components of the Discontinued LDA Stack Installing the Check Valves in the Discontinued LDA There are two types of check valves. The version of the Accela Pump that does not have an integrated dynamic mixer originally shipped with check valves that screwed into the lower displacement chamber.

  • Page 122: Installing The Check Valves And Holders

    Discontinued Versions of the Accela Pump Installing the Check Valves in the Discontinued LDA Installing the Check Valves and Holders This procedure describes how to install the current version of the check valves in the discontinued LDA.  To install the check valves and their holders 1.

  • Page 123: Installing The Screw-In Check Valves (Discontinued Version)

    Discontinued Versions of the Accela Pump Installing the Check Valves in the Discontinued LDA Installing the Screw-In Check Valves (discontinued version)  To install the check valves that screw into the lower displacement chamber 1. Install the PEEK film seals and check valves into the lower displacement chamber as...

  • Page 124: Installing The Components Of The Discontinued Lda Stack

    Discontinued Versions of the Accela Pump Installing the Components of the Discontinued LDA Stack 3. Remove the displacement chamber from the LDA body. Rotate it and repeat step Figure 75. Tightening the outlet check valve fitting Installing the Components of the Discontinued LDA Stack If they are installed correctly, the inner components of the LDA form a perfect stack that ensures smooth movement of the pistons.
  • Page 125 Discontinued Versions of the Accela Pump Installing the Components of the Discontinued LDA Stack Figure 76. Installing the stacked components of the LDA LDA body PEEK sealing rings Outlet module Upper displacement chamber Lower displacement chamber 4. Slide the compression lid into the upper bracket of the LDA body (see Figure 77).
  • Page 126 Discontinued Versions of the Accela Pump Installing the Components of the Discontinued LDA Stack 5. Using a 4 mm L-hex wrench, tighten the compression lid as shown in Figure Figure 78. Tightening the compression lid Accela Pump Hardware Manual Thermo Scientific...

  • Page 127: Index

    Accela Pump configuring the signal polarity settings instrument status page for the Accela Pump connecting to pump starting and stopping the solvent flow from the pump clamping screws, loosening...
  • Page 128 Super Flangeless low flow rates flow rate lower displacement chamber, installing minimum and maximum flow restrictor 47, 49, fuses blown part number and description replacing Accela Pump Hardware Manual Thermo Scientific...
  • Page 129 (figure) cleaning replacing components ruining contamination 43, 55, 84, secondary (figure) freezing soaking in methanol opening shipping container, contents problems sinker frit pulse dampener description solvent interconnect kit parts outlet priming Thermo Scientific Accela Pump Hardware Manual...
  • Page 130 Troubleshooting Guide tubing connecting pump to autosampler connecting the pump to the autosampler low-pressure solvent lines unpacking your pump upper displacement chamber, installing USB cable vacuum degasser description specifications Accela Pump Hardware Manual Thermo Scientific...

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