Flow Cell Flushing - Agilent Technologies InfinityLab LC Series User Manual

9 Maintenance and Repair

Flow Cell Flushing

Flow Cell Flushing

When If flow cell is contaminated
Tools required Description
Glass syringe
Adapter
Parts required #
1
1
Dangerous solvents
WAR N IN G
The strong solvents used in this procedure are toxic and flammable and
proper precautions are necessary.
Wear protective gloves and goggles.

Don't expose yourself to the vapors.

Aqueous solvents can build up algae and therefore should do not be left in the
NOTE
flow cell for long periods. Add a small percentage of organic solvents (e.g.
acetonitrile or methanol ~ 5 %).
The strong solvent should dissolve any potential contaminants in the flow cell.
NOTE
For example water for aqueous mobile phase buffers, chloroform or
tetrahydrofuran for not water soluble contaminants.
Do not exceed the flow cell pressure limit of 5 bar (0.5 MPa).
NOTE
In case the cell is contaminated, follow the procedure below.
1 Use the purge mode and flush with the strong solvent.
2 Leave this solution in the cell for about one hour.
3 Flush with mobile phase.
Agilent InfinityLab LC Series RID User Manual
Description
Strong solvent
Tubings to waste
134