Agilent Technologies InfinityLab LC Series User Manual page 109

8 Test Functions
Refractive Index Calibration
Preparing the Calibration
When
Recommended after exchange of the optical unit or RIM board.
Tools required Description
Laboratory balance
Parts required #
1
1
1
1
1
1 Preparation of the sucrose calibration solution.
2 Preparing the pump.
3 Flush the degasser and pump.
Agilent InfinityLab LC Series RID User Manual
p/n Description
DAB/Ph Eur/BP/JP/NF/USP Grade Sucrose
9301-1446 Syringe
9301-0407 Needle
5190-5111 Syringe filter, 0.45 µm, 100/pk
0100-1516 Fingertight fitting PEEK, 2/pk
a To prepare 25 ml of the calibration solution 87.5 mg of the Sucrose
sample is required.
b Add the weighed amount of sample into a suitable volumetric flask.
c Dispense 10 ml of LC grade water into the flask and shake or stir to
dissolve.
d Dilute the contents of the flask to volume with LC grade water.
Wait five minutes and shake again. The solution is now ready for use.
a Fill a suitable solvent bottle with LC grade water.
b Connect this bottle to Channel A of the pump, A1 if a binary pump.
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