Princeton Instruments PhotonMAX System Manual page 34

34 PhotonMAX System Manual
16-bit A/D is at full scale when the brightest parts of the image reach an
intensity of 65535. Adjust the aperture to where it is just slightly smaller
(higher f-stop) than the setting where maximum brightness on any part of
the image occurs.
•
Set the focus adjustment of the lens for maximum sharpness in the
viewed image.
3. After you have focused the camera, you can stop Focus mode, continue
Focus mode, begin Acquire mode, or wait for the CCD to reach the
operating temperature before going to Acquire mode.
Version 1.B