Accu-Scope EXC-120 Series Manual

EXC-120 Microscope Series
Phase Contrast Supplement
Alignment of the Phase Contrast Components
Components
100x Annulus
10x Annulus
Phase contrast consists of two components for each phase
contrast objective installed on the microscope:
Phase Retarder Ring inside each Ph objective. This gray
Phase ring is observed using the focusable Phase Centering
Telescope supplied with the system. In the following
instruction you will be advised when to substitute the
40x Annulus 20x Annulus
telescope for one of the microscope's eyepieces
Annulus Ring in the condenser. There is a different
Underside View of Phase Turret,
annulus for each objective—one ring corresponding to each
Variable Aperture Diaphragm
Ph objective. Its purpose is to shape the incoming light into
and 4 Annuli Holders, Adjustable (X-Y)
a hollow cylinder. The annuli are mounted in holders that are
individually movable in an X-Y manner— so as to be able to
align the image of each annulus with the gray phase ring in
each objective.
Installation
Install the Ph objectives on the microscope's nosepiece
starting with the 10x, and then rotate the nosepiece clockwise
and install the 20x, 40x and 100xR oil objectives in numerical
order.
Install the Phase Contrast Condenser/Turret into the
condenser carrier mounting ring and tighten the set screw
with the screwdriver that came with your microscope.
NOTE: The condenser mounting ring has been pre-centered
at the factory.
Select the B for brightfield position on the Condenser/Turret
by rotating it clockwise until the B clicks into place as shown.
Rotate the nosepiece and bring the 10x objective into
position.
Front Turret Installed and Positioned
Raise the Condenser/Turret to its highest position.
Currently in Optic Axis
Place a familiar "prepared, stained specimen" on the
stage and focus the 10x objective on this specimen.
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