Appendix A Troubleshooting; Introduction; Troubleshooting Files - illumina NextSeq 550Dx Instrument Reference Manual

Appendix A Troubleshooting

Introduction

Troubleshooting Files

Resolve Automatic Check Errors
Spent Reagents Container is Full
Rehybridization Workflow
BeadChip and Scan Errors
Custom Recipes and Recipe Folders
RAID Error Message
Configure System Settings
Introduction
For technical questions, visit the NextSeq 550Dx instrument support pages on the Illumina website. The
support pages provide access to documentation, downloads, and frequently asked questions.
Log in to your MyIllumina account for access to support bulletins.
For run quality or performance problems, contact Illumina Technical Support. See
on page 69.
Consider sharing a link to the run summary in BaseSpace with Illumina Technical Support to facilitate
troubleshooting.
Troubleshooting Files
An Illumina Technical Support representative might request copies of run-specific or scan-specific files
to troubleshoot issues. Typically, the following files are used for troubleshooting.
Troubleshooting Files for Sequencing Runs
Key File
Run information file
(RunInfo.xml)
Run parameters file
(RunParameters.xml)
RTA configuration file
(RTAConfiguration.xml)
InterOp files (*.bin)
Log files
Error log files
(*ErrorLog*.txt)
Global log files
(*GlobalLog*.tsv)
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
Folder Description
Root Contains the following information:
• Run name
folder
• Number of cycles in the run
• Number of cycles in each read
• Whether the read is an indexed read
• Number of swaths and tiles on the flow cell
Root Contains information about run parameters and run components. Information
folder includes the RFID, serial number, part number, and expiration date.
Root Contains the RTA configuration settings for the run.
The RTAConfiguration.xml file is created at the beginning of the run.
folder
InterOp Binary reporting files used for Sequencing Analysis Viewer.
InterOp files are updated throughout the run.
Logs Log files describe each step performed by the instrument for each cycle, and list
software and firmware versions used with the run. The file named
[InstrumentName]_CurrentHardware.csv lists the serial numbers of instrument
components.
RTA logs Log of RTA errors.
Error log files are updated whenever an error occurs.
RTA logs Log of all RTA events.
Global log files are updated throughout the run.
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Technical Assistance
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