User-Supplied Consumables And Equipment - illumina NextSeq 550Dx Instrument Reference Manual

Research mode
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NextSeq 550Dx Research Mode Instrument Reference Guide
Upon instrument initialization
u
When a run is started
u
When certain errors occur
u
When user interaction is required
u
When a run has finished
u
4
Select Save to save settings and exit the screen.
Set Run Setup Options
1
From the Manage Instrument screen, select System Customization.
2
Select the Use Advanced Load Consumables checkbox to enable the option to load all run
consumables from a single screen.
3
Select the Skip Pre-Run Check Confirmation checkbox to start sequencing or scanning
automatically after a successful automatic check.
4
Select Save to save settings and exit the screen.
Set Automatic Purge Option
1
From the Manage Instrument screen, select System Customization.
2
Select the Purge Consumables at End of Run checkbox to purge unused reagents from the
reagent cartridge to the spent reagents container automatically after each run.
NOTE
Purging consumables automatically adds additional time to the workflow.
3
Select Save to save settings and exit the screen.

User-Supplied Consumables and Equipment

The following consumables and equipment are used for consumables preparation, sequencing, and
instrument maintenance.
User-Supplied Consumables for Sequencing Runs
Consumable
1 N NaOH
(sodium hydroxide)
200 mM Tris-HCl, pH7
Alcohol wipes, 70% Isopropyl
or
Ethanol, 70%
Lab tissue, low-lint
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
Supplier
General lab supplier
General lab supplier
VWR, catalog # 95041-714
(or equivalent)
General lab supplier
VWR, catalog # 21905-026
(or equivalent)
Purpose
Library denaturation, diluted to 0.2 N
Library denaturation
Flow cell cleaning and general purpose
Flow cell cleaning and general purpose
13

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