Beadchip And Scan Errors - illumina NextSeq 550Dx Instrument Reference Manual

NextSeq 550Dx Research Mode Instrument Reference Guide
7 Select Next to proceed to the pre-run check and start the run.

BeadChip and Scan Errors

Software Cannot Read the BeadChip Barcode
When the barcode error dialog box appears, select from the following options:
Select Rescan. The software attempts to read the barcode again.
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Select text field and enter the numeric barcode as shown in the image. Depending on the
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BeadChip, barcode numbers have up to 12 digits. Select Save. The barcode image is stored in
the output folder.
Select Cancel. The imaging compartment door opens to unload the BeadChip adapter.
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BeadChip Scan Failure
Images are registered after they are scanned. Registration identifies beads by correlating locations on
the scanned image with information provided in the bead map, or DMAP folder.
Sections that fail registration are indicated in red on the BeadChip image.
Figure 28 BeadChip Showing Failed Sections
After the scan is complete and scanning data are written to the output folder, the Rescan button
becomes active.
When Rescan is selected, the software performs the following steps:
Rescans samples that contain failed sections using an increased tile-to-tile overlap.
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Generates output files in the original output folder.
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Overwrites previous output files for failed sections.
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Increments the scan counter by 1 for each rescan, but does so in the background. The software
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does not rename the output folder.
Rescan or Start New Scan
1 Select Rescan to scan samples that contain failed sections.
2 If the scan continues to fail, end the scan.
3 Remove the BeadChip and adapter and inspect the BeadChip for dust or debris. Use canned air or
other compressed dusting method to clear the debris.
4 Reload the BeadChip and start a new scan.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
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