Chapter 1 Overview; About This Guide; Introduction - illumina NextSeq 550Dx Instrument Reference Manual

Chapter 1 Overview

About this Guide

Introduction

Additional Resources
Instrument Components
Sequencing Consumables Overview
About this Guide
This instrument reference guide provides instructions on the use of the NextSeq 550Dx instrument in
research (RUO) mode.
Introduction
®
The Illumina NextSeq
between high-throughput sequencing and array scanning.
Sequencing Features
High-throughput sequencing—The NextSeq
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genomes, and transcriptomes and supports TruSeq
Flow cell types—Flow cells are available in configurations for high output and mid output. Each flow
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cell type is kitted with a compatible prefilled reagent cartridge.
Real-Time Analysis (RTA)—Integrated analysis software performs on-instrument data analysis,
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which includes image analysis and base calling. The NextSeq 550Dx uses an implementation of
RTA called RTA v2, which includes important architecture and feature differences. For more
information, see Real-Time Analysis on page
®
BaseSpace integration—The sequencing workflow is integrated with BaseSpace, the Illumina
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genomics computing environment for data analysis, storage, and collaboration. For instruments
configured for BaseSpace, library information and run parameters are specified on the BaseSpace
Prep tab. Runs that were set up in BaseSpace appear on the instrument interface during run setup.
As the run progresses, output files are streamed in real time to BaseSpace or BaseSpace Onsite.
Array Scanning Features
Integrated array scanning in control software—The NextSeq 550Dx instrument allows you to
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transition between array scanning and high-throughput sequencing on the same instrument using the
same control software.
Extended imaging capability—The imaging system in the NextSeq 550Dx instrument includes
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software and stage modifications that enable imaging of a larger surface area to accommodate
BeadChip scanning.
BeadChip types—Compatible BeadChip types include CytoSNP-12, CytoSNP-850K, and Karyomap-
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12.
BeadChip adapter—A reusable BeadChip adapter enables easy loading of a BeadChip onto the
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instrument.
Data Analysis—Use the BlueFuse
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Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
™
550Dx instrument is a single solution that provides a seamless transition
®
Multi software to analyze array data.
™
550 instrument enables sequencing of exomes, whole
™ ™
and Nextera libraries.
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