illumina NextSeq 550Dx Instrument Reference Manual page 42

NextSeq 550Dx Research Mode Instrument Reference Guide
Laboratory-grade water (125 ml)
u
5 Add 125 ml wash solution to the center reservoir of the buffer wash cartridge.
6 Select Perform Wash, and then select Manual Post-Run Wash.
Prepare for a Quick Wash
You can prepare for a quick wash as described below as an alternative to
Run Wash on page 36.
User-Supplied
Consumables
100% Tween 20
Laboratory-grade water
1 Combine the following volumes to result in a 0.05% Tween 20 wash solution:
100% Tween 20 (20 µl)
u
Laboratory-grade water (40 ml)
u
2 Add 40 ml wash solution to the center reservoir of the buffer wash cartridge.
3 Select Perform Wash, and then select Quick Wash.
Load a Used Flow Cell and the Wash Cartridges
1 If a used flow cell is not present, load a used flow cell. Select Load, and then select Next.
2 Remove the spent reagents container and discard the contents in accordance with applicable
standards.
WARNING
This set of reagents contains potentially hazardous chemicals. Personal injury can
occur through inhalation, ingestion, skin contact, and eye contact. Wear protective
equipment, including eye protection, gloves, and laboratory coat appropriate for risk of
exposure. Handle used reagents as chemical waste and discard in accordance with
applicable regional, national, and local laws and regulations. For additional environmental,
health, and safety information, see the SDS at support.illumina.com/sds.html.
3 Slide the empty spent reagents container into the buffer compartment until it stops.
4 Remove the used buffer cartridge from the previous run, if present.
5 Load the buffer wash cartridge containing wash solution.
6 Remove the used reagent cartridge from the previous run, if present.
7 Load the reagent wash cartridge.
8 Select Next. The prewash check begins automatically.
Start the Wash
1 Select Start.
2 When the wash is complete, select Home.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
Volume and Description
Used to make 40 ml 0.05% Tween 20 wash solution
Loaded onto buffer wash cartridge (center reservoir)
Prepare for a Manual Post-
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