illumina NextSeq 550Dx Instrument Reference Manual page 28

NextSeq 550Dx Research Mode Instrument Reference Guide
2 Select Next.
3 Confirm run parameters.
Run Name—Name of the run as assigned in BaseSpace.
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Library ID—Name of the pooled libraries as assigned in BaseSpace.
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Recipe—Name of the recipe, either NextSeq High or NextSeq Mid depending on the reagent
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cartridge used for the run.
Read Type—Single Read or Paired End.
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Read Length—Number of cycles for each read.
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[Optional] Custom Primers, if applicable.
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4 [Optional] Select the Edit button to change run parameters. When finished, select Save.
Run parameters—Change the number of reads or number of cycles per read.
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Custom primers—Change the settings for custom primers. For more information, see NextSeq
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Custom Primers Guide (document # 15057456) .
Purge consumables for this run—Change the setting to purge consumables automatically after
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the current run.
5 Select Next.
Enter Run Parameters (Standalone Configuration)
1 Enter a run name of your preference.
2 [Optional] Enter a library ID of your preference.
3 Select a read type, either Single Read or Paired End.
4 Enter the number of cycles for each read in the sequencing run.
Read 1—Enter a value up to 151 cycles.
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Index 1—Enter the number of cycles required for the Index 1 (i7) primer.
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Index 2—Enter the number of cycles required for the Index 2 (i5) primer.
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Read 2—Enter a value up to 151 cycles. This value is typically the same number of cycles as
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Read 1.
The control software confirms your entries using the following criteria:
Total cycles do not exceed the maximum cycles allowed
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Cycles for Read 1 are greater than the 5 cycles used for template generation
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Index Read cycles do not exceed Read 1 and Read 2 cycles
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5 [Optional] If you are using custom primers, select the checkbox for the primers used. For more
information, see NextSeq Custom Primers Guide (document # 15057456) .
Read 1—Custom primer for Read 1.
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Index 1—Custom primer for Index 1.
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Index 2—Custom primer for Index 2.
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Read 2—Custom primer for Read 2.
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6 [Optional] Select the Advanced Settings
From the Recipe drop-down list, select a recipe. Only compatible recipes are listed.
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Output folder location—Change the output folder location for the current run. Select Browse to
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navigate to a network location.
Included file—Select files to include in the Output Folder that can be useful if further analysis is
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required. For example, manifest files and sample lists.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
button to change run parameters.
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