Calibration And Adjustment - Thermo Scientific Finnpipette F1 Instructions For Use Manual

Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fi ll the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the
knob to the fi rst stop, making sure the tip is well
below the surface.
2. Release the knob slowly to the ready position.
This will fi ll the tip. Keep the tip in the solution.
3. Depress the knob to the fi rst stop and release
slowly. Keep repeating this procedure until the
interior wall of the tip is clear.
4. Finally, depress the knob all the way to the
second stop to completely empty the tip.

Calibration and adjustment

All Finnpipettes are factory calibrated and adjusted to give the volumes as specifi ed with
distilled or deionized water using the forward pipetting technique. It should be noted that the
use of other pipetting techniques may affect the calibration results. The pipettes are constructed
to permit re-adjustment for other pipetting techniques or liquids of different temperature and
viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:
Volume range
under 10 μl
10-100 μl
above 100 μl
Test liquid: Water, distilled or deionized, "grade 3" water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C
to 30°C. The relative humidity must be above 50%. Especially with volumes under 50 μl the
air humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
Procedure to check calibration
The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is fi rst pre-wetted 3-5 times and a series of ten pipettings is done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Use of
forward pipetting technique is recommended. The maximum permissible errors are designed for
forward method. It is recommended to calibrate all channels of the multichannel pipette, but a
suitable method is also to calibrate only the edge channels. Adjustment check prior calibration is
done using one of the middle channels. It is recommended to use the ISO8655 calibration limits
presented in the following tables or user-defi ned specifi cations.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
readable graduation
0.00 1 mg
0.01 mg
0.1 mg
8
1 2 3 4 5