Calibration And Adjustment - Thermo Scientific F1-ClipTip 4641170 Instructions For Use Manual

Picture 6
When the pipette is not used for a period of time, the plunger movement may become stiff due to
hardening of grease. In rare cases this can affect the functionality of the super blow-out piston,
which can become temporarily jammed. To ensure proper functionality it is recommended to press
the plunger several times to the fi nal stop (step 3 in picture) before dosing the sample liquid. This
releases the piston and re-distributes the lubricant.
NOTE! If the super blow-out piston is jammed, increased force might be required to release it. Ensure
that plunger moves to the down position (Picture 6).
For more information about different pipetting techniques, check the Good Laboratory Pipetting
Guide available at www.thermoscientifi c.com/GLP.
5.6 Tip ejection
To release the tip, point the pipette at a suitable waste receptacle and press the tip ejector with
your thumb.

6. Calibration and Adjustment

6.1 Factory calibration limits
F1-ClipTip pipettes are factory calibrated and adjusted to give the volumes as specifi ed with distilled
or deionized water using the forward pipetting technique. Manufacturer specifi cations are shown on
the pipette calibration certifi cate and in Appendix 2.
Cautions for calibration and pipette adjustment
It should be noted that the use of different pipetting techniques may affect the calibration
results. The pipettes are constructed to permit re-adjustment for other pipetting techniques
or liquids of different temperature and viscosity.
The performance of a new and serviced pipette must be checked and documented.
The manufacturer's specifi cations should be used as guidelines and the user should establish
acceptable imprecision and inaccuracy performance limits (ISO 8655).
The user determines the performance and applicability of the pipette and tip combination
for a particular application and determine the required interval for performance checking.
Before starting the actual dosing, pre-rinse the tip with the liquid by fi lling and emptying the
tip three to fi ve times to improve accuracy and precision.
6.2 Test conditions and equipment
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:
Volume range
under 10 μl
10 100 μl
–
above 100 μl
The test liquid is deionized distilled "grade 3" water in compliance with ISO 3696. Tests are done in a
draft-free room at a constant (±0.5°C) temperature between 15°C to 30°C. The relative humidity must
be above 50%. Especially with volumes under 50 μl, air humidity should be as high as possible to reduce
the effect of evaporation loss. Special accessories, such as an evaporation trap, are recommended.
Plunger in up position
Readable graduation
0.001 mg
0.01 mg
0.1 mg
Plunger in down position
9