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B.1.6
Glucose
Glucose measurement is based on the level of H
produced during the enzymatic reaction between glucose
and oxygen molecules in the presence of the glucose oxidase
enzyme. The reaction is described by the following equation:
Glucose + O
At a constant potential of 0.70 volts, electroactive H
oxidized at the surface of the platinum anode as follows:
H
O
––––––––> 2H
2
2
The current generated by the flow of electrons at the
surface of the platinum sensor is proportional to the glucose
concentration of the sample.
B.1.7
Lactate
Lactate measurement is based on the level of H
during the enzymatic reaction between lactate and oxygen
molecules in the presence of the lactate oxidase enzyme.
The reaction is described by the following equation:
Lactate + O
At a constant potential of 0.70 volts, electroactive H
oxidized at the surface of the platinum anode as follows:
H
O
––––––––> 2H
2
2
The current generated by the flow of electrons at the
surface of the platinum sensor is proportional to the lactate
concentration of the sample.
Glucose Oxidase
––--–––––––––––> Gluconic acid + H
2
+
Lactate Oxidase
–––––––––––––> Pyruvate acid +
2
H
O
2
+
-
+ O
+ 2e
2
2
-
+ O
+ 2e
2
Appendix B
O
2
2
O
2
2
Equation 9
O
is
2
2
Equation 10
O
produced
2
2
Equation 11
O
is
2
2
Equation 12
B-5
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