Related Manuals for Thermo Scientific Centra-W Cell Washer
Summary of Contents for Thermo Scientific Centra-W Cell Washer
- Page 1 SERVICE MANUAL SM2392 Revision 1 Centra-W Cell Washer Cat. No. 2392 -- For 100/120/220/240 VAC, 50/60 Hz...
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Page 2: Table Of Contents
4.3 Guard Bowl Removal ....................21 4.4 Pump Tubing Replacement ..................21 4.5 Decontamination ......................23 4.6 Cover Interlock Bypass ....................24 WARRANTY ........................25 5.1 Condition Of Returned Equipment ................25 6 SPECIFICATIONS ......................26 Centra-W Cell Washer Service Manual... - Page 3 This manual contans WARNINGS against operating procedures which could result in an accident and/or personal injury. It also contains CAUTIONS against procedures which could result in damage to your centrifuge or accessory equipment. Read this manual thoroughly before operating or servicing this centrifuge. Centra-W Cell Washer Service Manual...
- Page 4 2-1 Unpacking ....................5 2-2 Voltage Selection ..................7 3-1 Centra-W Wash Cycle ................11 3-2 Rotor And Plunger Installation ..............14 4-1 Pump Assembly ..................21 4-2 Cover Interlock Bypass ................23 Chapter 13 Drawings ..................43 Centra-W Cell Washer Service Manual...
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Page 5: Description
1 DESCRIPTION The Model Centra-W Cell Washer is manufactured by the International Equipment Company. This benchtop cell washer is used to automatically perform the washing phase of the anti-globulin procedure in tests using up to six drops of blood sample. The cell washer will accommodate either twelve 12 x 75 mm tubes, or twelve 10 x 75 mm tubes. -
Page 6: Installation
(Have someone hold the carton, if necessary). Place the cell washer on a sturdy, level bench near a waste container or drain to receive decanted saline. Centra-W Cell Washer Service Manual... - Page 7 Complete the warranty registration card and return it to IEC. Figure 2-1 Unpacking Centra-W Cell Washer Service Manual...
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Page 8: Power Configuration
From the following table, determine the correct fuses. Insert two fuses in the fuse drawer and replace the fuse drawer in the power entry module. Make sure it locks into place. Voltage Fuse Cat No. 100V/120V 250V 5A T 49946 220V/230V/240V 220V/240V 250V 2.5A T 49937 Centra-W Cell Washer Service Manual... -
Page 9: Power Cord
Periodically inspect the drain tubing to ensure its proper operation. If the drain line becomes kinked or restricted, drained liquid can back up the drain hose and into the motor compartment causing motor failure. Centra-W Cell Washer Service Manual... -
Page 10: Operation
This key is used to power the cover solenoid and open the cover. The indicator to the left of this key will light whenever the Power Switch is in the On position and the rotor is stopped. Press the OPEN COVER key to release the cover. Centra-W Cell Washer Service Manual... - Page 11 This indicator lights when the saline solution reservoir is depleted. An alarm also sounds. The cell washer defaults to the check mode, allowing the user to hand-fill the tubes, and to prime the pump system. Pressing the RUN key will resume the run. Centra-W Cell Washer Service Manual...
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Page 12: Description Of Operation
“stepping" motion of the rotor, cell pellets are disrupted (broken up) for resuspension of the cells during subsequent wash cycles. If desired, the agitation sequence may be manually added at the end of the run. Centra-W Cell Washer Service Manual... - Page 13 Figure 3-1 Centra-W Wash Cycle Centra-W Cell Washer Service Manual...
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Page 14: Operational Precautions
Routine housekeeping and cleaning practices will help to remove salt deposits in, and prolong the life of, these components. Priming Priming is required whenever a new saline supply is connected. To prime the instrument: Centra-W Cell Washer Service Manual... -
Page 15: Volume Calibration
Recheck by following this procedure starting at step 5. Repeat this procedure as necessary. The cell washer must be Calibrated for a 12 tube load even though fewer tubes may be used. Centra-W Cell Washer Service Manual... -
Page 16: Wash Mode
Agitate). If the STOP key was used, then the wash cycle will start from the beginning when restarted. NOTE: If saline flow is interrupted or depleted, the run stops, an alarm sounds and the ADD SALINE LED lights. (See Section 3.4) Centra-W Cell Washer Service Manual... -
Page 17: Spin Mode
NOTE: Agitation can be performed with the cover open or closed. Press the AGITATE key to obtain a six-second agitation. An agitate can be added to the spin cycle by pressing the SPIN and AGITATE keys. Centra-W Cell Washer Service Manual... -
Page 18: Operating Procedure
Therefore, simply press the WASH key or SPIN key. Then press the RUN key. The run cycle will include all the previously entered number of wash cycles or spin time, and agitate, if programmed. Centra-W Cell Washer Service Manual... -
Page 19: Anti-Human Globulin
5. Add one (1) drop of Coombs control cells to each negative tube. 6. Agitate tubes and spin for 15 seconds. Examine for agglutination. All tubes should give positive reactions. Centra-W Cell Washer Service Manual... -
Page 20: Maintenance
Reconnect the saline supply line to the saline reservoir and prime the unit using the CALIBRATE key. Make certain that the inner bowl is wiped out to remove any excess moisture before resuming use of the cell washer. 2. Clean the rotor and solenoid plunger. Centra-W Cell Washer Service Manual... -
Page 21: Three Months
Press the SPIN key and enter the length of spin time. Press RUN. As soon as the spin cycle starts (start of spin can be determined by listening for hum), start the stopwatch. Stop the stopwatch when the time display reads 000. Verify that spin time is correct. Centra-W Cell Washer Service Manual... -
Page 22: Replacement Parts
Insert the pump rollers into the pump head (long end of shaft outward). Turn the pump shaft by hand to verify that the pump motor is coupled. Centra-W Cell Washer Service Manual... - Page 23 Gently pull on the drain tubing (to unkink it), and slide its clamp up the tubing as close to the cabinet assembly as possible. Tighten it in place to prevent kinking. Figure 4-1 Pump Assembly Centra-W Cell Washer Service Manual...
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Page 24: Decontamination
National Committee for Clinical Laboratory Standards (NCCLS) guidelines for the protection of laboratory workers from instrument biohazards. 3. Wipe instrument with a damp towel (water only) and thoroughly dry. Centra-W Cell Washer Service Manual... -
Page 25: Cover Interlock Bypass
POWER switch to the OFF position. Place an insulated rod approxmately 6 inches straight through the opening. Push gently to disengage the latch. Replace the cover plug and turn the POWER switch back to ON. Figure 4-2 Cover Interlock Bypass Centra-W Cell Washer Service Manual... -
Page 26: Warranty
The RGA paperwork includes a certificate for you to sign indicating that you have performed these steps. IEC will not accept the shipment unless this signed certificate accompanies it. You must prepay transportation to the service depot. Centra-W Cell Washer Service Manual... -
Page 27: Specifications
AMBIENT TEMPERATURE: Operation 10 °C to 35 °C Storage 20 °C to 70 °C RELATIVE HUMIDITY: Operation 20% to 90% Non-Operation 5% to 95% Storage 5% to 95% *NO LOAD Specifications Subject To Change Without Notice Centra-W Cell Washer Service Manual... -
Page 28: Service
Once a component has been identified as defective, IEC recommends replacement. Over the history of the IEC Centra-W Cell Washer there have been many improvements. This manual is specific to the current production version at the time of writing of this manual. Generally, the information contained in this service portion should be applicable. -
Page 29: Troubleshooting
Check cover splash guard alignment. Large amount of saline Check drain tubing for restrictions. remains in guard bowl. Ensure drain tubing flows downward -drain receptacle below cell washer. Cover won't open. Check solenoid. Checck hinge tension. Centra-W Cell Washer Service Manual... -
Page 30: Cabinet
PCB to the control panel. Take care not to lose the standoffs when lifting the PCB off. Latch Assembly The latch assembly contains three parts; the latch striker (catch), solenoid Centra-W Cell Washer Service Manual... -
Page 31: Hinges
(each) mounting screws, repositioning the hinge and securing it. Hinge tension is adjusted from beneath the hinge by turning the adjustment screw (clockwise to tighten). Take care to have the same tension in both hinges. Centra-W Cell Washer Service Manual... -
Page 32: Cover Assembly
There are several components mounted to the base assembly. These include the power entry module, power switch, line filter, transformer, relay, diode, terminal board, mounting feet and capacitors (3). These components may be accessed by removing the cabinet (see Section 8.1). Centra-W Cell Washer Service Manual... -
Page 33: Power
The circuit board has 4 interface connectors, labelled J1 through J4. Con- nector J3 brings power to the circuit board. The other connectors distribute power and signals as follows: Membrane Control Panel Saline & Cover Switches Power Out Centra-W Cell Washer Service Manual... - Page 34 J4. Power the unit back on. If a fuse does not blow, reconnect connectors, one at a time, in the above manner until a fuse blows. Search for the short in the last component connected before the fuse blew. Centra-W Cell Washer Service Manual...
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Page 35: Circuit Board
LED is not lit, then the board should be re- placed. LED indicators are present for the following devices: Motor - High Speed Motor - Low Speed Pump Relay Decant Solenoid Cover Solenoid Centra-W Cell Washer Service Manual... -
Page 36: Fuse
The fuse is rated as follows: 6.3 A, 125 V 5x20mm Slo-Blo IEC Part No. 8976 10.4 Test Points The board contains 3 test points. They are as follows: Ground Logic Voltage 10 Hz Frequency Centra-W Cell Washer Service Manual... -
Page 37: Drive Assembly
DRIVE ASSEMBLY 11.1 General The motor in the Centra-W Cell Washer is a two speed brushless induction motor with sealed, permanently lubricated bearings. The drive assembly has added sealing at the factory to prevent saline from reaching the motor. If motor replacement is necessary, IEC recommends replacement of the drive assembly. -
Page 38: Brake
11.3 Brake The brake in the Centra-W Cell Washer is an electric brake. The PC board, first, powers the low speed winding to bring the speed down and then applies a DC voltage to the high speed winding to stop the motor. The brake is used after the third (DECANT) step of each WASH cycle and during the agitate step. -
Page 39: Rotor Repair
Remove the second retaining ring (shown #15), and remove the three socket head screws (shown #13) using a 7/64" Allen wrench. Lift the metal deck (shown #4) off of the assembly. Centra-W Cell Washer Service Manual... - Page 40 Place the stand on the shaft as shown and replace the last retaining ring in the groove of the shaft. Place rotor right side up & verify that all tubeholders swing out fully as shown. Figure 11-1 Rotor Assembly (see Section 13 for parts identification) Centra-W Cell Washer Service Manual...
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Page 41: Rotor Dependant Parts
Cell washers with higher serial numbers can be modified in the field. In addition to the new rotor (IEC Part No. 477), a new guard bowl (IEC Part No. 50402) and solenoid plunger (IEC Part No. 44919) must be installed. Centra-W Cell Washer Service Manual... -
Page 42: Pump
PUMP 12.1 General The peristaltic saline pump system in the Centra-W Cell Washer consists of three parts: the pump motor, pump head and pump tubing. Replacement of the pump tubing is described in Section 4.4. 12.2 Pump Motor With the unit unplugged, there are electrical characteristics of the pump motor that can be checked. -
Page 43: Flow Switch
The switch is normally open (saline present). When saline is not present the switch closes and stops a run during the fill mode. To replace the switch, remove the cabinet (see Section 8.1), disconnect the wiring harness to the switch and remove its two mounting screws. Centra-W Cell Washer Service Manual... -
Page 44: Drawings
Note: At the time of publication these drawings and diagrams were accurate. Changes to the units do occur. If you have any questions regarding these drawings and diagrams, please contact IEC Technical Support at (800) 843-1113. Centra-W Cell Washer Service Manual... - Page 45 43173 Transformer Diode 61235 61534 Line Filter (not shown) Relay 43176 61056 Capacitor Splash 60935 Shield Power 49321 Switch 55418 Foot (4) 33340 Pump Control Motor Panel 49318 PC Board Capacitors (2) 50001 44443 60551 Centra-W Cell Washer Service Manual...
- Page 46 Ring 50249 Drain Extension 65579 Inner Splash Guard Solenoid Coil 40539 49994 Coil Mounting Plate (below) Outer 40269 Tubing Shaft Seal 43346 50394 Inner Tubing 41636 Motor/Drive Assy. 50304 Mount (3) 49354 *See Section 11.6 Centra-W Cell Washer Service Manual...
- Page 47 Figure 13-3 Pump Assembly Coupler 50279 Flow Switch 49777 Tubing Tubing 50287 50286 Tubing Elbow 65451 40076 Cube Adapter 50259 Clamp Valve 65450 Pump Head Assembly 49317 Centra-W Cell Washer Service Manual...
- Page 48 Qty. Part No. Tubeholder 44924 Stand 44945 Shaft 44910 Deck 44907 Pivot Ring 44906 Filler Head 44920 Filler Head Cap 3666 Filler Tube 44904 Dowel Pin 44903 O ring 36211 Pivot 44921 Reflective Tape 50310 Centra-W Cell Washer Service Manual...
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