Thermo Scientific Cryofuge 16 Instruction Manual
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Thermo Scientific Cryofuge 16 Instruction Manual

Heavy duty water cooled centrifuge
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Thermo Scientific
Cryofuge 16
Heavy Duty Water Cooled Centrifuge
Instruction Manual
50152622-h • 03 / 2021
Visit us online to register your warranty:
thermofisher.com/labwarranty

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Summary of Contents for Thermo Scientific Cryofuge 16

  • Page 1 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuge Instruction Manual 50152622-h • 03 / 2021 Visit us online to register your warranty: thermofisher.com/labwarranty...
  • Page 2 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked with the following symbol:...

  • Page 3: Table Of Contents

    Intended Use for Cryofuge 16 Heavy Duty Water Cooled . . . . . . . . . . . . . . . .
  • Page 4 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges 4 . Maximum Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .III–8 Use of Tubes and Consumables ...................III–9...
  • Page 5 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A–1 1 .
  • Page 6 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Table of Figures Figure I–1: Centrifuge clearance, example with barcode scanner .......... I–2 Figure III–1: Overview centrifuge and rotor, e.g. HAEMAFlex 12 ...........III–2 Figure III–2: Align windshield knob and drive shaft .............III–3 Figure III–3: Identify the circular marking, e.g.
  • Page 7 Table V–1: Error messages ....................V-3 Table A–1: Technical Data Cryofuge 16 Heavy Duty Water Cooled ........A–1 Table A–2: Directives, Standards and Guidelines Cryofuge 16 Heavy Duty Water Cooled ..A–2 Table A–3: Refrigerants used in the Cryofuge 16 Heavy Duty Water Cooled ......A–2 Table A–4: Mains Supply ....................A–3...

  • Page 8: Preface

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Preface Preface Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty.

  • Page 9: Intended Use For Cryofuge 16 Heavy Duty Water Cooled

    Trained personnel can be a clinical laboratory technologist, a medical laboratory technician or personal with similar education. Associated Rotors and Components The Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled centrifuge can be operated with the Thermo Scientific ™ ™...

  • Page 10: Precautions

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Preface Precautions Signal Word and Color Degree of Hazard WAR NIN G Indicates a hazardous situation that, if not avoided, could result in death or serious injury. CAU T I ON Indicates a hazardous situation that, if not avoided, could result in minor or moderate injury.

  • Page 11: Preparation

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Preface Preparation WARNING It is the obligation of the operator to make sure, that the proper protective clothing is used. Mind the “Laboratory Biosafety ƒ Manual” of the World Health Organization (WHO) and the regulations in your country.

  • Page 12: Operating

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Preface Operating WARNING Make sure that the power supply cable is set up correctly. Electrical Hazard can occur if the power supply cable is not ƒ set up correctly. Hang the power supply cable in loops on the cable holder at the backside of the centrifuge. The flag attached to the power supply cable is marking the end of the necessary part of the power supply cable that needs to hang in loops on the cable holder.

  • Page 13: Symbols Used On The Centrifuge

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Preface Symbols used on the Centrifuge This symbol refers to general hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. CAUTION means that material damage could occur.

  • Page 14: Symbols Used In The Manual

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Preface Make sure that the power supply cable is set up correctly. Electrical Hazard can occur if the power supply cable is not set up correctly. Hang the power supply cable in loops on the cable holder at the backside of the centrifuge.

  • Page 15: Transport And Set Up

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Transport and Set Up THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. Transport and Set Up Make sure that the centrifuge and the packaging is not damaged. Speak to the shipping CA UT I O N company and Thermo Fisher Scientific immediately if damage is found.

  • Page 16: Figure I-1: Centrifuge Clearance, Example With Barcode Scanner

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Transport and Set Up THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. ƒ Keep a safety zone of minimum 30 cm (Figure I–1 on page I–2, green area) around the centrifuge.

  • Page 17: Transporting

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Transport and Set Up THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. 2. Transporting WARN I N G Never stand before a moving centrifuge to stop it. If the centrifuge is moved on an uneven surface, it can get faster due to its own weight.

  • Page 18: Thermo Scientific

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Thermo Scientific Centri-Touch User Interface Thermo Scientific Centri-Touch User Interface ➀ Actual status information ➁ Set values ➂ Settings and navigation II–1...

  • Page 19: Status

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Thermo ScientificCentri-Touch User Interface Status The upper part of the touchscreen displays the centrifuge status. The remaining time is indicated while centrifugation is in progress. The progress bar shows the actual phase of the centrifugation.

  • Page 20: Run Parameters

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Thermo ScientificCentri-Touch User Interface Run Parameters The set points for centrifugation can be set in the parameterization window. Clicking one of these buttons will open a new window in which you can input the appropriate set point value.

  • Page 21: Lighthouse Mode

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Thermo ScientificCentri-Touch User Interface Lighthouse Mode If no entry is made via the main screen in 30 seconds, the unit changes to lighthouse mode. In this mode, the status, current values and progress display are displayed larger.

  • Page 22: Operation

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation I I I Operation For detailed instructions and information refer to the separate manual for the Thermo Scientific ™ Centri-Touch ™ User Interface. 1. Switch on the Centrifuge Push the switch at the right side.

  • Page 23: Install A Rotor

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation 3. Install a Rotor WARN I N G If you have not installed the rotor correctly, do not operate it. Make sure that the rotor is not damaged. Do not use damaged rotors.

  • Page 24: Figure Iii-2: Align Windshield Knob And Drive Shaft

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation CAU T I ON If you have not installed the rotor correctly, do not operate it. Make sure that the rotor is not damaged. Do not use damaged rotors. Make sure that the centrifugation chamber is free from objects.

  • Page 25: Figure Iii-4: Rotor Locking Tool

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation To prevent possible damage to rotor or centrifuge, clean the threads of the drive shaft. 20360104 Figure III–4: Rotor locking tool. Figure III–5: Put the rotor locking tool. Put the rotor locking tool (20360104) into the opening of the guidung pin into the rotor locking screw.

  • Page 26: Figure Iii-6: Tighten The Rotor To The Drive Shaft

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation Figure III–6: Tighten the rotor to the drive shaft. Clean and lubricate the rotor trunnion bolts and the two bolt seats on the sides of each bucket (“Cleaning” on page IV–3).

  • Page 27: Figure Iii-9: Correctly Assembled Round Bucket

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation Figure III–8: Install buckets into rotor, e.g. HAEMAFlex 12 When assembling round buckets insert the O-ring below the thread in the bucket. Do not insert it to the bucket lid.

  • Page 28: Figure Iii-10: Correct Loading

    Operation Correct Loading ✓ Figure III–10: Correct Loading Incorrect Loading ✕ Figure III–11: Incorrect Loading Put the windshield lid into the holder at the inner side of the centrifuge door. The Thermo Scientific Auto-Lid function ™ ™ automatically opens and closes the windshield when the centrifuge door opens and closes.

  • Page 29: Maximum Loading

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation Figure III–12: Insert windshield lid into holder CA UT I O N Always put the windshield lid into the holder at the inner side of the centrifuge door. Make sure the rotor is locked properly.

  • Page 30: Use Of Tubes And Consumables

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation Use of Tubes and Consumables Care should be taken to ensure that the tubes and bottles used in the centrifuge are: ƒ rated to or above the selected RCF to be spun at, ƒ...

  • Page 31: Runtime Preselection

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation Explanation of RCF-Value The relative centrifugal force (RCF) is given as a multiple of the force of gravity (g). It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of the type of device.

  • Page 32: Programs

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation 7. Programs For detailed instructions and information refer to the according manual for the Thermo Scientific Centri-Touch User Interface. The instructions stated here are just an extract of how to work with programs. Press on the touchscreen.

  • Page 33: Centrifugation

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation 8. Centrifugation WA R N I N G Do not operate the centrifuge on its casters. WA R N I N G Make sure that the power supply cable is properly hanging on the cable holder on the back of the centrifuge.

  • Page 34: Remove A Rotor

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Operation 9. Remove a Rotor WA R N I N G Risk of cuts through fast rotating windshield lid. Do not open the centrifuge door while the rotor assembly spins. Only when the rotor assembly has stopped, use the mechanical emergency centrifuge door release.

  • Page 35: Maintenance And Care

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Maintenance and Care Maintenance and Care 1. Cleaning Intervals For the sake of personal, environmental, and material protection, you must clean and if necessary disinfect the centrifuge and its Components on a regular basis.

  • Page 36: Rotor And Components Inspection

    Press Settings (gears symbol), then Runs and then Rotor Log to see the information. Besides the values of the rotor an information about the number of cycles completed by this rotor type in this centrifuge is shown. For detailed instructions and information refer to the separate manual for the Thermo Scientific CentriTouch User Interface. IV–2...

  • Page 37: Cleaning

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Maintenance and Care 3. Cleaning CAU T I ON Before using any cleaning methods, users should check with the manufacturer of the cleaning agents that the proposed method will not damage the equipment.

  • Page 38: Touchscreen

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Maintenance and Care Clean the bucket surfaces and rotor bolts as follows: Cut a cleaning pad (66309) into 12 mm (1/2 inch) wide strips. Saturate one of the pad strips with acetone or alcohol.

  • Page 39: Filter Mat

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Maintenance and Care 3. 2. Filter Mat CA UT I O N Do not touch the edges of the housing with your hands when the grid is removed. Cutting damage can occur if you touch the edges due to their sharpness.

  • Page 40: Disinfection

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Maintenance and Care 4. Disinfection WA R N I N G Do not touch infected parts. Hazardous infection is possible when touching the contaminated rotor and centrifuge parts. Infectious material can get into the centrifuge when a tube breaks or as a result of spills.

  • Page 41: Autoclaving

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Maintenance and Care 6. Autoclaving CA UT I O N Never exceed the permitted temperature and duration when autoclaving. No chemical additives are permitted in the steam. N OT I C E Always disassemble all parts before autoclaving, e.g.

  • Page 42: Maintenance

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Maintenance and Care 7. Maintenance 7. 1. Preventive Maintenance In order to keep this product able to perform the intended application in a reliable and safe state, ongoing preventive maintenance is necessary in accordance with the following recommended schedule: ƒ...

  • Page 43: Shipping And Disposal

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Maintenance and Care 8. Shipping and Disposal WA R N I N G When removing the centrifuge and Components from use for disposal you have to clean and if necessary disinfect or decontaminate the entire system. In doubt contact the Thermo Fisher Scientific customer service.

  • Page 44: Troubleshooting

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Troubleshooting Troubleshooting 1. Mechanical Emergency Door Release Serious injuries can occur, if you touch a spinning rotor with your hands or tools. WA R N I N G A rotor can still be spinning after a power failure occurs.

  • Page 45: Ice Formation

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Troubleshooting Pull out the power supply plug. On both sides of the housing are two white plastic plugs. Get these two white plastic plugs out of both sides of the housing.

  • Page 46: Troubleshooting By Guide

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Troubleshooting 3. Troubleshooting by Guide CAU T I ON If problems occur other than those listed in this table, service technician must be contacted. If an error occurs, follow the instructions on the touchscreen. Error messages have a red colored background.

  • Page 47: Information For The Customer Service

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Troubleshooting 4. Information for the Customer Service If you need to contact a service technician, please provide the order no. and the serial no. of your centrifuge. This information can be found on the back near the inlet for the power supply cable.

  • Page 48: Technical Specifications

    Weight 200, 208, 220, 230, 240 V, 479 kg (1 056 lb) 50 / 60 Hz 1 m in front of the instrument at 1.6 m height. Without rotor. Table A–1: Technical Data Cryofuge 16 Heavy Duty Water Cooled A–1...

  • Page 49: Directives, Standards And Guidelines

    IEC 61010-2-101 3 IEC 61326-1 Class B IEC 61326-2-6 EN 62304 EN 62366 EN ISO 14971 EN ISO 13485 Table A–2: Directives, Standards and Guidelines Cryofuge 16 Heavy Duty Water Cooled 2. Refrigerants Centrifuge Refrigerant Quantity Pressure Thermo Scientific Cryofuge R-449A 1.79 kg...

  • Page 50: Mains Supply

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Technical Specifications 3. Mains Supply The following data is to be taken into consideration, when selecting the mains connection socket. Power Mains Voltage Rated Current Building Fuse Equipment Frequency (Hz) Consumption Fuse (A) 27.0...

  • Page 51: Components

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Technical Specifications 5. Components Rotor Article No. Description Max. Speed Max. RCF Capacity 75007740 Sample Tracking Kit 75007741 Networking Access Kit 75003862 HAEMAFlex 12 Swinging Bucket Rotor 12 x 500 ml...

  • Page 52: Rotors

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Rotors Rotors Items Supplied for Rotors Article No. Item Quantity Rotor (incl. Windshield Bowl and Windshield Lid) 75003862 Thermo Scientific HAEMAFlex 12 75003882 Thermo Scientific HAEMAFlex 16 Rotor Locking Tool 20360104...

  • Page 53: Haemaflex 12 Rotor

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Rotors HAEMAFlex 12 Rotor with Double Blood Bag Buckets Technical Data Cryofuge 16 Heavy Duty Water Cooled Centrifuge Voltage 200, 208, 220, 230, 240 V Rotor Capacity 12 x 1000 ml...

  • Page 54: Haemaflex 16 Rotor

    Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Rotors HAEMAFlex 16 Rotor with Double Blood Bag Buckets Technical Data Cryofuge 16 Heavy Duty Water Cooled Centrifuge Voltage 200, 208, 220, 230, 240 V Rotor Capacity 16 x 1000 ml...
  • Page 55 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–1...
  • Page 56 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–2...
  • Page 57 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–3...
  • Page 58 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–4...
  • Page 59 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–5...
  • Page 60 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–6...
  • Page 61 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–7...
  • Page 62 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–8...
  • Page 63 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–9...
  • Page 64 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–10...
  • Page 65 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Chemical Compatibility Chart C–11...
  • Page 66 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges Index Mains Connection I–3 Mains Supply A–3 Maintenance Maintenance and Care IV–1 Maximum Loading III–8 Mechanical Emergency Door Release Metal Parts IV–2 Acceleration / Deceleration Profiles III–9 Aerosol-tight Rotors III–9 Autoclaving IV–7...
  • Page 67 1434 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/centrifuge © 2016-2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont.

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