Processing - Color-Coded Projection - Zeiss Lightsheet Z.1 Operating Manual

Lightsheet Z.1
3.3.3 Processing – Color-coded Projection
 standard
Tool accepts Z-stacks and time series (incl. combinations with several other
dimensions). See full description of tool for details.
The Color-coded projection function generates a
maximum intensity projection image along the z-,
or time- dimension of a multidimensional data set
(Fig. 129). Instead of using the colors assigned to
the channels, it displays the position in z or in time
with a color gradient.
• To
open the
Processing tool, select it from the list in the
Processing main tab.
• Click on the triangle buttons to show or hide
the Method Parameters and Preview panels
of the function.
• Select the Input Image with the Select button
and choose the dimension along which you
want to generate a color-coded projection from
the pull down menu (z or time) in the Method
Parameters panel.
• Select one of the two color gradients by
marking the checkbox.
gradients the corresponding depth or time is
indicated in grey numbers and letters.
• Define the range (ROI) of the Z-Stack or time
series to be used by using the sliders or the
input boxes with arrows.
• Clicking the Apply button creates the color-
coded projection as a new image document
using the name shown in the Output image
display for the Select field.
02/2013
CHAPTER 4 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
Licensing
optional
Color-coded projection
Below the
000000-1790-528
Instrument
 LSM  ELYRA
color
Fig. 129
 Lightsheet Z.1
Processing – Color-coded projection
Carl Zeiss
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