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jenway Genova Nano Operating Manual

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Genova Nano Spectrophotometer
Operating Manual
737 555 REV D/08-16

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Summary of Contents for jenway Genova Nano

  • Page 1 Genova Nano Spectrophotometer Operating Manual 737 555 REV D/08-16...
  • Page 2 Safety Please read this information carefully prior to installing or using this equipment. 1. The unit described in this manual is designed to be operated only by trained personnel. Any adjustments, maintenance and repair must be carried out as defined in this manual, by a person qualified to be aware of the hazards involved.
  • Page 3 2. Il est essentiel que les personnes utilisant et intervenant sur cet appareil respectent les règles de sécurité de travail, en plus des instructions détaillées précisées dans ce manuel. 3. En-dehors des éléments décrits dans les procédures de maintenance ci-incluses, cet appareil ne contient aucun élément réparable par l'utilisateur.
  • Page 4 Entfernen von Abdeckungen und Versuche von hierfür unqualifiziertem Personal, Anpassungen oder Wartungsarbeiten durchzuführen, haben zur Folge, dass die Garantie verfällt und können zusätzliche Reparaturkosten auslösen. 4. Es ist jederzeit auf die sicherheitsrelevanten Daten sämtlicher verwendeter Chemikalien Bezug zu nehmen. Allgemein anerkannte Labormethoden zum sicheren Umgang mit Chemikalien sollten eingesetzt werden.
  • Page 5 personale non qualificato invaliderà la garanzia. In questi casi, sarà necessario pagare un importo per le riparazioni effettuate. 4. È sempre necessario fare riferimento ai dati sulla salute e sulla sicurezza forniti con le sostanze chimiche utilizzate. Adottare le procedure di laboratorio generalmente accettate per la gestione delle sostanze chimiche.
  • Page 6 de laboratorio de aceptación generalizada para la manipulación segura de productos químicos. No utilice sustancias peligrosas o inflamables en el instrumento. 5. Si existe la sospecha de que las medidas protectoras de seguridad han quedado dañadas en cualquier modo, la unidad debe inutilizarse y protegerse contra toda operación que se intente llevar a cabo.

  • Page 7: Table Of Contents

    Contents SECTION 1 - INTRODUCTION ................9 INSTRUMENT DESCRIPTION..................9 GENOVA NANO WITH MICRO VOLUME ACCESSORY SPECIFICATION ....9 UNPACKING........................10 SECTION 2 – ACCESSORY LAYOUT AND INSTALLATION ......11 ACCESSORY LAYOUT ....................11 INITIALISATION ......................11 Note: The instrument will return to the last main menu used....... 11 SECTION 3 –...
  • Page 8 11.1.1 Calibration Procedure Error Codes ................27 11.1.2 Verification Check Error Codes ..................27 11.2 TROUBLESHOOTING GUIDE ..................28 11.3 TECHNICAL SUPPORT ....................28 SECTION 12 – GLOSSARY OF ICONS ............. 29 SECTION 13 – CHEMICAL COMPATABILITY ..........30...

  • Page 9: Section 1 - Introduction

    SECTION 1 - INTRODUCTION INSTRUMENT DESCRIPTION The Genova Nano is a UV/visible spectrophotometer dedicated to life science analysis. This spectrophotometer incorporates a micro volume sample measurement accessory that allows sample volumes as low as 0.5µl to be analysed. In addition to the standard measurement modes:...

  • Page 10: Unpacking

    Genova Nano instruction manual (737 555) • Genova Plus instruction manual (736 505) • The Genova Nano is delivered with the micro volume accessory securely packaged in the spectrophotometers sample chamber. Note: The protective packaging must be removed before the instrument is first initialised.

  • Page 11: Section 2 - Accessory Layout And Installation

    The instrument will initially check for firmware updates and then perform several power-on tests before displaying the main menu: Fig 2.2 – Genova Nano Main Menu Note: The instrument will return to the last main menu used.

  • Page 12: Section 3 - Micro-Volume Settings

    SECTION 3 – MICRO-VOLUME SETTINGS ACCESSING THE MICRO VOLUME SETTINGS The micro volume icon is displayed in the bottom right hand corner of the screen in each measurement mode. Micro Volume Icon Fig 3.1 – Accessing the Micro Volume Settings Menu The micro volume settings are accessed by pressing the key below the micro volume icon.

  • Page 13: Path Length Selection

    PATH LENGTH SELECTION 3.2.1 Known Path Length Measurements If the required measurement path length is known it can be selected by pressing the button adjacent to the 0.2mm or 0.5mm path length setting icons. The selected setting is indicated by an icon with a black background. Once the required path length setting has been selected press the button adjacent to the tick icon to confirm.

  • Page 14: Section 4 - Performing Micro Volume Measurements

    SECTION 4 – PERFORMING MICRO VOLUME MEASUREMENTS For detailed descriptions of the measurement modes that are available on the Genova Nano spectrophotometer please refer to the supplied Genova Plus operating manual. PIPETTING SAMPLES ONTO THE MICRO VOLUME READ HEAD The Genova Nano spectrophotometer is designed to measure sample volumes ranging from 0.5µl to 5.0µl.

  • Page 15: Sample Recovery Or Removal

    SAMPLE RECOVERY OR REMOVAL Once a measurement is complete, the sample solution can be recovered from the lower read head with a suitable pipette or removed from the read heads by cleaning with a suitable lint free cloth. 1. Open the lid of the spectrophotometer. The read head mechanism will open to allow access to the upper and lower read heads...

  • Page 16: Section 5 - Top 10 Tips For Sample Measurement

    0.001 equates to a derived concentration change of 1µg/ml at 0.5mm path length (based on 1 A260 unit of dsDNA = 50µg/ml). 10. Jenway recommends that the micro volume accessory is calibrated every 6 months A set of calibration solutions (Part code 035 092) are supplied with the Genova Nano...

  • Page 17: Section 6 - Step By Step Guide To Making A Dna Measurement

    260nm, the units are µg/ml and F1 = 50. 3. Lift the lid of the Genova Nano and pipette 2µl of water onto the read head. Close the lid. Perform the Blank Reading: 4.

  • Page 18: Multi-Wavelength Mode

    5. Lift the lid of the Genova Nano and pipette 2µl of water onto the read head. Close the lid. Perform the blank reading. 6. Lift the lid and wipe the water from both the upper and lower read heads.

  • Page 19: Section 7 - Calibration Of The Micro Volume Accessory

    SECTION 7 – CALIBRATION OF THE MICRO VOLUME ACCESSORY Jenway recommends that the micro volume accessory is calibrated every 6 months. A set of calibration solutions (Part code 035 092) are supplied with the Genova Nano spectrophotometer. Please note that the calibration solutions should be discarded 1 week after being opened.

  • Page 20: Calibration And Verification

    2. Select the digit to be changed using the keys at the bottom of the screen. Use the keys adjacent to the Arrow icons to increase or decrease the number. Press the key adjacent to the Tick icon to save any changes. 3.
  • Page 21 During the calibration process, there is the option to log the calibration data to USB stick. This generally isn’t needed, but if you experience difficulties in performing a successful calibration you can forward the calibration log data to techsupport@bibby-scientific.com for further advice. By default, the calibration data logging is disabled.
  • Page 22 5. If the measured values are within the required tolerances, the Passes Test icon will be displayed. Press the button adjacent to the Tick icon to continue or the button adjacent to the Cross icon to abort the calibration process. The Calibration Solution Measurement icon will be displayed.
  • Page 23 12. The unit takes three readings at 260 and 330nm, at both 0.5mm and 0.2mm path lengths. At the end of the readings, the unit will display the results of the measurements taken: A330.5 0.006 [0.050] A330.2 0.006 [0.050] A.5 0.5349(0.4899), A.2 0.2306(0.1959) 13.
  • Page 24 19. The unit takes two readings at 260 and 330nm, at both 0.5mm and 0.2mm path lengths. At the end of the readings, the unit will display the results of the measurements taken: A260.2 -0.024 [-0.040 to 0.000] A330.2 -0.021 [-0.040 to 0.000] A260.5 -0.010 [-0.040 to 0.000] A330.5 -0.009 [-0.040 to 0.000] 20.

  • Page 25: Section 8 - Cleaning And Decontamination

    SECTION 8 – CLEANING AND DECONTAMINATION CLEANING Wiping the sample from both the upper and lower read heads upon completion of each sample measurement with a lint free cloth is usually sufficient to prevent sample carryover and avoid residue buildup. Although generally not necessary, water aliquots can be used to clean the measurement surfaces after the measurement of particularly highly concentrated samples to ensure no residual sample is retained on either read head.

  • Page 26: Section 9 - Accessories

    10.2 SERVICE Our dedicated service team are on hand to help in the unlikely event that your Jenway equipment develops a fault. Please contact them by one of the following means stating the serial number of the unit and a clear description of the problem: E-mail: service@bibby-scientific.com...

  • Page 27: Section 11 - Troubleshooting

    SECTION 11 – TROUBLESHOOTING 11.1 CALIBRATION ERROR CODES If an error code is displayed during calibration it will be accompanied by a Clipboard icon and a number to indicate the cause of the error. The first time that an error is displayed, it will be possible to repeat the erroneous measurement.

  • Page 28: Troubleshooting Guide

    11.3 TECHNICAL SUPPORT Jenway have a dedicated Technical Support team made up of experienced scientists who are on hand to help with any applications advice and questions you may have about our products or how to use them. If you require any technical or application assistance please contact the team at: E-mail: techsupport@bibby-scientific.com...

  • Page 29: Section 12 - Glossary Of Icons

    SECTION 12 – GLOSSARY OF ICONS Description Mode ICON Back key Common Tick icon - Done/yes Common Cross icon – Cancel/no Common Arrow icon – Move down, decrease Common Arrow icon – Move up, increase Common Settings 0.2mm path length setting / selected Settings 0.5mm path length setting / selected Settings...

  • Page 30: Section 13 - Chemical Compatability

    SECTION 13 – CHEMICAL COMPATABILITY Assay Chemical Concentration Sodium bicinchoninate Sodium carbonate Sodium tartrate 0.16%* Sodium hydroxide 0.1M* Sodium bicarbonate 0.95%* Copper (II) sulphate 0.08% Biuret Sodium potassium tartrate 0.9%* Biuret Copper (II) sulphate 0.3%* Biuret Potassium iodide 0.5%* Biuret Sodium hydroxide 0.08M Lowry...
  • Page 31 Bibby Scientific Ltd Beacon Road Stone Staffordshire ST15 0SA United Kingdom Tel: +44 (0)1785 812121 e-mail: info@bibby-scientific.com www.bibby-scientific.com...

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