jenway 7300 Operating Manual
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jenway 7300 Operating Manual

Spectrophotometer
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Model 7300 and 7305
Spectrophotometer
Operating Manual
730 005 REV D/08-14

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  • Page 1 Model 7300 and 7305 Spectrophotometer Operating Manual 730 005 REV D/08-14...

  • Page 3: Safety

    Safety Please read this information carefully prior to installing or using this equipment. 1. The unit described in this manual is designed be operated only by trained personnel. Any adjustments, maintenance and repair must be carried out as defined in this manual, by a person qualified to be aware of the hazards involved.
  • Page 4 Bitte lesen Sie diese Hinweise vor Installation oder Gebrauch dieser Ausrüstung sorgfältig durch. 1. Das in diesem Handbuch beschriebene Gerät darf nur von geschultem Personal bedient werden. Alle Anpassungen, Wartungsarbeiten und Reparaturen müssen entsprechend der Vorgaben in diesem Handbuch und von einer kompetenten Person, die mit den damit verbundenen Gefahren vertraut ist, durchgeführt werden.
  • Page 5 5. Nel caso in cui si sospetti che la salute possa essere pregiudicata in qualsiasi modo, disattivare l'unità per renderla inutilizzabile. Qualsiasi condizione di errore deve essere immediatamente segnalata al responsabile per la manutenzione. Lea esta información atentamente antes de instalar o utilizar este equipo. 1.

  • Page 6: Table Of Contents

    Contents Safety ......................... 3 Contents ........................6 SECTION 1 - Introduction ..................8 INSTRUMENT DESCRIPTION..................... 8 INSTRUMENT SPECIFICATION..................8 SECTION 2 – Installation ................... 9 UNPACKING......................... 9 INSTALLATION ........................9 DISPLAY ..........................10 CONTROLS ........................11 REAR PANEL ........................12 FRONT PANEL ........................
  • Page 7 6.4.2 Measuring a Sample After Calibrating to a Factor ............. 28 POST MEASUREMENT OPTIONS ..................28 6.5.1 Changing Concentration Units .................... 28 6.5.2 Changing the Concentration Factor ..................29 6.5.3 Changing the Calibration Standard Value ................29 SECTION 7 – PRINTING AND AUTOLOGGING ............. 30 PRINTING ...........................

  • Page 8: Section 1 - Introduction

    SECTION 1 - Introduction INSTRUMENT DESCRIPTION The 7300 and 7305 spectrophotometers are suited to a wide range of applications in education, quality control, environmental and clinical analysis. The 7300 is a visible spectrophotometer covering a wavelength range from 320nm to 1000nm. The 7305 is a UV/Visible spectrophotometer with a wavelength range from 198nm to 1000nm.

  • Page 9: Section 2 - Installation

    SECTION 2 – Installation UNPACKING Remove the 7300 or 7305 from the packaging and ensure the following items are included: 1. Model 7300 spectrophotometer (730 001), or Model 7305 spectrophotometer (730 501) 2. 24V 65W power supply unit (021 060) 3.

  • Page 10: Display

    The instrument will perform several power on tests before displaying the main menu: Fig 2.2.2 – All Power On Tests Complete 1. Instrument check – ensures the validity of the saved parameters 2. Dark test 3. Checks for the accessory fitted. If an active accessory is found the instrument verifies communication and response 4.

  • Page 11: Controls

    CONTROLS The keypad used for these models enables an easy and effective way of navigating the different measurement modes, entering numbers, saving and analysing results. The soft keys are active when an icon is displayed above or adjacent to the key. The only exception to this is the back key which is always active.

  • Page 12: Rear Panel

    REAR PANEL The image below shows the rear panel on the instrument: Fig. 2.5.1 – Rear Panel 1. Lamp access panel Allows access to lamp when replacement is necessary 2. Power switch On/off switch for the unit 3. Power in socket Connection socket for power supply unit 4.

  • Page 13: Section 3 - Theory And Practice Of Spectroscopy Measurements

    SECTION 3 – Theory and Practice of Spectroscopy Measurements THEORY OF SPECTROSCOPY MEASUREMENT UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample.

  • Page 14: Spectroscopy Measurement

    The optical layout of the 7300 and 7305 spectrophotometers is shown below: Figure 3.2.1 – Diagram of light path The light from the pre-focused tungsten halogen (7300) or pre-aligned xenon (7305) lamp is focused onto the grating, with 1200 lines per millimeter, which separates the light into discreet wavelengths.

  • Page 15: Good Practice Guidelines

    GOOD PRACTICE GUIDELINES 1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambient temperature and light levels should remain as constant as possible. 2. If required adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP) should be monitored with regular calibration checks and a suitable Quality Control (QC) programme.
  • Page 16 10. Cuvettes and sample holders must be filled to a minimum level which covers the light path. All Jenway spectrophotometers have a beam height of 15mm. 11. The instrument must be calibrated to zero absorbance/100% transmittance prior to taking...

  • Page 17: Section 4 - Instrument Setup

    SECTION 4 – Instrument Setup NAVIGATING AND SCREEN SETUP The main menu screen is displayed below. Fig 4.1 – Home Screen To navigate around the spectrophotometer screen press the soft keys adjacent to icons displayed on the screen. In the main menu either of the two soft keys adjacent to the measurement mode icon can be pressed to access the mode.

  • Page 18: Time And Date

    TIME AND DATE The time and date menu enables the current time and date to be set. This information will be saved on all results and displayed on printouts. The time and date menu can be accessed from the main menu by holding the key below the time and date icon for 2 seconds.

  • Page 19: Screen Contrast

    LAMP SAVE This function is only available on the 7300 visible spectrophotometer which uses a tungsten halogen lamp. The lamp save function enables the time in minutes to be set after which the lamp will be turned off following a period of no lamp activity, i.e.
  • Page 20 The time set will begin to count down when there is no lamp activity. When the count down is complete the lamp and the fan will be turned off and the lamp save icon is shown in all the measurement modes. To bring the instrument out of the lamp save in order to perform a measurement press the key below the lamp save icon.

  • Page 21: Section 5 - Photometrics

    SECTION 5 – PHOTOMETRICS The photometrics measurement mode enables simple measurements of absorbance and % transmittance to be performed. The sample is measured at one wavelength and at one point in time. There are no post measurement calculations available in this measurement mode. MODE SPECIFIC PARAMETERS The photometrics operating menu enables measurement parameters to be changed.

  • Page 22: Calibration

    CALIBRATION The calibration must be performed at the same wavelength at which the sample will be measured. Insert a cuvette containing the blank solution into the sample chamber and close the instrument lid. Press the key below the calibrate to zero absorbance icon. This sets the instrument to zero absorbance and 100% transmittance.

  • Page 23: Section 6 - Concentration

    SECTION 6 – CONCENTRATION The concentration measurement mode enables simple measurements of absorbance and concentration to be performed. In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor. The sample is measured at one wavelength at one point in time.

  • Page 24: Settings

    The settings menu is accessed through the operating menu by pressing the key adjacent to the settings icon. In the settings menu press the key below the wavelength icon. This will open a number entry screen. Use the keys at the bottom of the screen to select the digit to be adjusted.

  • Page 25: Selecting Concentration Units

    6.2.2.1 Selecting Concentration Units The units of concentration can be selected from a number of options: no units, %, ppm, EBC, SRM, mEq/l, mEq, M, mM, µM, nM, U, U/l, U/ml, g/l, mg/l, µg/l, ng/l, g/dl, mg/dl, µg/dl, mg/ml, µg/ml, ng/ml, µg/µl, ng/µl, mol/l, mmol/l.

  • Page 26: Using A Factor

    6.2.2.4 Using a Factor The factor menu enables a factor to be entered. This function is accessed by pressing the key adjacent to the factor icon. This opens the extended number entry screen. Use the keys at the bottom of the screen to select the digit to be changed.

  • Page 27: Calibrating To A Factor

    Press the key below the calibrate to zero absorbance or standard icon, this will open another menu with the option to re-calibrate to zero absorbance or to calibrate to the previously entered standard value. Press the key adjacent to the calibrate to standard icon. If the standard selected requires a factor beyond the range of the instrument the check standard icon will be displayed.

  • Page 28: Measuring A Sample After Calibrating To A Factor

    6.4.2 Measuring a Sample After Calibrating to a Factor Remove the cuvette containing the blank solution and place a cuvette containing the sample to be measured in the sample chamber. Close the instrument lid and press the key below the measure to factor icon. Once the measurement is complete the concentration and absorbance values are displayed.

  • Page 29: Changing The Concentration Factor

    Once a measurement is complete, press the key adjacent to the settings icon, then press the key below the units icon. This will either cycle through the available unit options or open the units selection page. In the units selection page, select the updated measurement unit from the available options and press the key adjacent to the tick icon to confirm.

  • Page 30: Section 7 - Printing And Autologging

    SECTION 7 – PRINTING AND AUTOLOGGING The utility toolbar in the operating menu provides access to printing, print setup options, and autologging options. Print/print settings Autolog menu Fig 7.1 - Operating Menu PRINTING The utility toolbar in the operating menu enables results to be printed and print setup options to be set.

  • Page 31: Printing Results

    The destination of the printouts can be the internal printer or an external serial printer. The results can only be sent to an external serial printer if there is a serial printer connected to the instrument via the RS232 serial port. Press the key adjacent to the computer icon to select the external printer. The results can only be sent to the internal printer if there is an internal printer connected.

  • Page 32: Selecting Result's Destination

    To set the time period between each measurement press the key below the timer icon and use the keys adjacent to the arrow icons to increase or decrease the time in 1 second intervals. To reset the time to one second press and hold the key below the timer icon for 2 seconds.

  • Page 33: Connecting To A Pc

    CD drive. If the PC software does not auto run open My Computer and double click on the Jenway 73 series software icon. Follow the instructions to install the PC software to the required location. Refer to the PC software manual for further instructions. Once the software is installed, turn the mains power on to the instrument.

  • Page 34: Section 8 - Accessories And Spare Parts

    SECTION 8 – Accessories and Spare Parts OPTIONAL ACCESSORIES Part Code Description of Accessory 660 101 Internal printer 735 401 Automatic 8 cell turret 735 201 Sipper pump 735 301 Peltier 735 701 Combined sipper Peltier pump 735 801 10x10mm path length cuvette holder 735 901 16/24mm test tube holder 736 001...

  • Page 35: Passive Accessories

    Squeeze the grey plastic clips together so that the printer top opens. Slot the printer into the top of the instrument and push down until it fits flush to all four sides. Insert the paper roll into the printer – ensuring that there is some paper sticking out of the printer before clicking the grey plastic back into place.
  • Page 36 The water heated cuvette holder is already fitted to a base plate so the base plate in the instrument must be removed before installation. Unscrew screws 1 to 4 and lift out the metal base plate. For this accessory as well as removing the passive accessory base plate, the front panel of the instrument must also be removed.
  • Page 37 Once the base plate and front panel have been removed insert the water-heated cuvette holder into the chamber, ensuring the base plate rests flat in the unit. Replace the screws 1 to 4, securing the accessory in place. Fit the custom front panel to the unit ensuring the metal pipes on the accessory are aligned and fed through the two holes in the front panel.

  • Page 38: Active Accessories

    From the rubber tubing supplied, cut two shorter lengths of tubing and use these to connect the internal metal pipes of the cell block (7) to the internal metal pipes on the outer casing (8). Using two separate pieces of rubber tubing connect the two external metal tubes (9) to an external water bath and pump.

  • Page 39: Automatic 8 Cell Turret

    This will expose the bottom of the sample chamber with the power supply connection needed to operate the active accessories. 8.2.3.1 Automatic 8 cell turret Take the 8 cell turret base plate. Connect the power supply in the bottom of the sample chamber to the connector on the underside of the base plate.

  • Page 40: Peltier

    8.2.3.2 Peltier For this accessory as well as removing the passive accessory base plate, the front panel of the instrument must also be removed. Loosen screws 5 and 6 until the front panel can be lifted out in the forwards direction. Take the Peltier base plate.

  • Page 41: Sipper Pump

    8.2.3.3 Sipper pump For this accessory as well as removing the passive accessory base plate, the front panel of the instrument must also be removed. Loosen screws 5 and 6 until the front panel can be lifted out in the forwards direction. Take the sipper base plate.
  • Page 42 For sipping: 1. Connect the sipper pump tubing to the outlet port on the flow-through cuvette. 2. Secure the tubing using the clip on the righthand side of the pump head. 3. Ease the tubing round the rollers by carefully rotating them clockwise, by hand.

  • Page 43: Combined Sipper Peltier Pump

    secured, ensure the tubing is routed into the two retaining clips located on the base plate at the side of the pump head. 5. Fit the other end of the tubing onto the outlet port, located on the inside of the front bulkhead. 6.

  • Page 44: Using The Accessories

    8.3 USING THE ACCESSORIES 8.3.1 Automatic 8 cell turret When the automatic 8 cell turret is in use the 8 cell turret icon is displayed in the bottom right hand corner of the screen. The current cell position is displayed adjacent to the 8 cell turret icon.

  • Page 45: Sipper Pump

    This opens the Peltier settings screen. Use the keys at the bottom of the screen to select the digit to be changed and use the arrow icons to increase or decrease the number. The temperature can be set in °C or °F by pressing the key adjacent to the °C icon. Repeat presses will cycle between °C and °F.

  • Page 46: Manual Sipper Pump Settings

    8.3.3.1 Manual Sipper Pump Settings To operate the sipper pump in manual mode press the key adjacent to the manual sipper icon. Select the preferred pump direction by pressing the key below the forwards or backwards arrow. Press the key adjacent to the tick icon to save and return to the operating menu.

  • Page 47: Timed Sipper Pump Settings

    8.3.3.2 Timed Sipper Pump Settings To operate the sipper pump in timed mode press the key adjacent to the timed sipper pump icon. Press the key below the calibrate timed sipper icon. Select the required pump direction by pressing the key below the forwards or backwards arrow.
  • Page 48 This stage allows an air gap to be added to the calibration sequence. If an air gap is not required press the key below the 001 icon to set the air gap to one. If a previously programmed air gap is to be used press the key adjacent to the double greater than icon to skip this stage and retain the current air gap time.
  • Page 49 To perform a measurement place the sipper tubing into the sample and press the key below the sipper pump icon. Confirmation will be needed to start the sipper pump. Press the key adjacent to the cross icon to cancel and return to the operating menu.

  • Page 50: Combined Sipper Peltier Pump

    8.3.4 Combined sipper Peltier pump When the combined sipper Peltier is in use the sipper Peltier icon is displayed in the bottom right hand corner of the screen. The current temperature is displayed above the set point temperature adjacent to the sipper Peltier icon.

  • Page 51: Section 9 - Maintenance And Service

    9.2.1 Tungsten Halogen Lamp Replacement This option is only valid for 7300 spectrophotometers. Before replacing the lamp disconnect the unit from the mains supply and ensure the lamp is cool before handling. Access to the tungsten halogen lamp can be gained via the lamp access panel located on the rear of the instrument (refer to section 2.5).

  • Page 52: Xenon Lamp Module Replacement

    Decontamination Certificate. This is available as a downloadable pdf file at www.jenway.com, or contact us and we will send you a copy. Please clearly mark the package for the attention of the Service Department and post to the following address:...

  • Page 53: Section 10 - Troubleshooting

    (caution icon) or fatal (stop icon). If the error is fatal contact your local distributor or Jenway service department (refer to section 9.3). If the error is a warning it may be possible to retry the test. In this case a back icon will also be displayed. The table below shows the error codes:...
  • Page 54 Err 5 Light Saturation Not Found This error indicates that the peak light hasn’t been found at zero. The most likely causes of this error are: 1. Lamp failure. 2. Deteriorating lamp signal. Fatal 3. Sample or cuvette in the sample holder. Solution: Ensure the sample holder is empty.

  • Page 55: Troubleshooting Guide

    10.3 TECHNICAL SUPPORT Jenway have a dedicated Technical Support team made up of experienced scientists who are on hand to help with any applications advice and questions you may have about our products and how to use them. If you require any technical or application assistance please contact the team at: E-mail: jenwayhelp@bibby-scientific.com...

  • Page 56: Section 11 - Declaration Of Conformity

    SECTION 11 – Declaration of Conformity...
  • Page 58 SECTION 12 – Glossary of Icons Mode ICON Description Common Back key Common Tick icon - Done/yes Cross icon – Cancel/no Common Common Printer icon - Print/open printer settings Common No results to send to printer Common Computer icon - RS232 serial port for connection to an external printer or a computer Common No results to send to RS232...
  • Page 59 Main Menu Opens concentration measurement mode Main Menu Opens photometrics measurement mode Main Menu Instrument settings Main Menu 12.00 Time/date icon Time & Date Clock icon - Set time Time & Date Calendar icon - Set date Toggle icon – Switches date format Time &...
  • Page 60 Concentration 0.000 ABS Calibrate to zero absorbance Concentration Calibrate to a standard 0.000 Concentration Calibrate to zero absorbance or standard Concentration Factor menu Concentration Standard menu Concentration Resets standard or factor value to one Concentration Check standard Accessories No single cell - Method created on a unit with a single cell accessory fitted Accessories 8 cell turret icon –...
  • Page 61 Accessories Timed sipper pump calibration sequence Accessories Start sipper pump – enables take up time to be set Accessories Skip take up time – uses the previously set take up time Accessories Stop sipper pump Accessories 001 – sets air gap to one Accessories Reduce sample uptake/reduce air gap Accessories...
  • Page 62 Bibby Scientific Ltd Bibby Scientific France SAS Bibby Scientific Ltd. – Singapore Beacon Road Stone ZI du Rocher Vert - BP 79 Prudential Tower, Level 26, Staffordshire ST15 0SA 77793 Nemours Cedex 30 Cecil Street 049712 United Kingdom France Singapore Tel: +44 (0)1785 812121 Tel: +33 1 64 45 13 13 Tel: +65 6631 2976...

This manual is also suitable for:

7305

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