jenway 6405 Operating Manual
  1. Manuals
  2. Brands
  3. jenway Manuals
  4. Measuring Instruments
  5. 6405
  6. Operating manual

jenway 6405 Operating Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

MODELS 6400/6405
SPECTROPHOTOMETERS
OPERATING MANUAL
640 050/REV B/11-99

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the 6405 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for jenway 6405

Summary of Contents for jenway 6405

  • Page 1 MODELS 6400/6405 SPECTROPHOTOMETERS OPERATING MANUAL 640 050/REV B/11-99...

  • Page 2: Table Of Contents

    MODELS 6400/6405 SPECTROPHOTOMETERS OPERATING MANUAL CONTENTS SAFETY SECTION 1 INTRODUCTION Instrument Description Instrument Specification SECTION 2 INSTALLATION Unpacking Installation Controls Inputs/Outputs SECTION 3 OPERATION Operating Overview Instrument Set-up Menu Photometrics Spectrum Mode Multi-Wavelength Mode Kinetics Mode Quantitation Mode Good Practice Guidelines...
  • Page 3 SECTION 8 WARNING AND ERROR MESSAGES Messages relating to Power On Self Test Photmetrics, Spectrum, Multi-Wavelength, Kinetics and Quantitation Modes Photometrics Mode Spectrum Mode Multi-Wavelength Mode (please refer to a) Kinetics Mode Quantitation Mode Accessories EC Declaration of Conformity 640 050/REV B/11-99...

  • Page 4: Safety

    SAFETY Please read this information carefully prior to installing or using this equipment. The units described in this manual are designed to be operated only by trained personnel. Any adjustments, maintenance or repair must be carried out as defined in this manual by a person qualified to be aware of the hazards involved.

  • Page 5: Introduction

    This manual has been produced to help guide you through the many operating modes and features available on the 6400 and 6405. Our hope is that operation of the units is such that reference to this manual will be required only rarely. It is our aim to ensure that all information needed to operate these products satisfactorily is contained within.

  • Page 6: Instrument Specification

    Resolution: 0.001/0.01/0.1/1 Units: ppm, mg/l , g/l , M, %, blank Factor: 0.000 to 9999.99 Wavelength Range: 320 to 1100nm (6400) 190 to 1100nm (6405) Resolution: 0.1nm Accuracy: ±1.0nm Bandwidth: Maximum Scan Speed: 1400nm/min Readout: 240x128 pixel reflective LCD Outputs:...

  • Page 7: Installation

    2.2 INSTALLATION The 6400 / 6405 are designed to operate on 230V or 115V (50/60Hz) mains supply. The standard 2 metre cable supplied with the unit is fitted with an IEC type connector which can be plugged directly into the POWER IN socket on the rear panel.

  • Page 8: Controls

    Icons The following icons may appear on the instrument display during operation. UV lamp on (6405 only) This icon will flash during pre-strike warm up. Tungsten Halogen lamp alight. If the lamp(s) fail the symbol will not be shown.

  • Page 9: Inputs/Outputs

    2.4 INPUTS / OUTPUTS Fig. 2.4.1 Rear Panel Layout 1. LAMP ACCESS PANEL This panel allows the user to gain access to the lamps when replacement is necessary. NOTE: The lamp access panel and all ventilation slots must not be covered or obstructed at any time.

  • Page 10: Operation

    SECTION 3 OPERATION 3.1 OPERATING OVERVIEW This section has been provided as an overview of the product functions. For specific instructions on each mode of operation please refer to the appropriate section as detailed in the Contents section at the front of this manual.
  • Page 11 Spectrum This mode allows samples to be scanned between two user set wavelengths at 5nm, 1nm or 0.2nm intervals. The highest scan rate is attained by scanning at 5nm intervals. Scan statistics which list peaks and valleys can be displayed. An optional peak and valley filter can be employed which only lists peaks and valleys that exceed a percentage of the difference between the maximum peak and the minimum valley.
  • Page 12 Kinetics This mode allows kinetics rate measurements to be made. Absorbance or transmittance versus time is displayed graphically. The user can enter the kinetics lag time and run time. Alternatively, the kinetics run time can be set to commence when the absorbance or transmittance exceeds or falls below a user set level.

  • Page 13: Instrument Set-Up Menu

    3.2 INSTRUMENT SET-UP MENU Language Allows selection of the instrument operating language. Instrument ID Number Allows entry of a value between 1 and 999999 that appears on all printouts. This can be used to provide identification of the instrument. Printer Form Feed Advances paper in the in-built printer by four lines.
  • Page 14 UV Lamp Save... Allows access to the UV lamp save menu. Enable Turns the lamp save feature on or off. When on, the deuterium lamp will only be switched on when the wavelength (or scan range in spectrum mode) falls below the wavelength set in this menu. When off, the deuterium lamp is permanently on.

  • Page 15: Photometrics

    3.3 PHOTOMETRICS MODE Having selected the Photometrics Mode from the Main Menu options the following display will be shown: It is recommended that set-up parameters be reviewed prior to calibration or measurement to ensure the selected measurement mode and values are correct. Select SET UP mode using the key and press MEASURE MODE...
  • Page 16 Offset Allows the zero absorbance concentration to be offset by the value entered. The offset is set to zero by calibration to a standard. Resolution The maximum resolution that concentration readings are displayed to can be set up to three decimal places.

  • Page 17: Spectrum Mode

    3.4 SPECTRUM MODE Having selected the Spectrum Mode from the Main Menu options the following display will be shown: Spectrum Mode Pre-Scan Menu A blank graph is displayed, using the current settings for measurement mode, the y-axis range and the scan wavelength range.
  • Page 18 Set Up Menu Each parameter can then be set using the keys and pressing to confirm value entered. Measure Mode Selects between transmittance or absorbance for the scan y-axis. Start Wavelength The wavelength at which the scan will commence. End Wavelength The wavelength at which the scan will finish.
  • Page 19 Noise Filter The post-scan statistical feature will list all turning points (peaks and valleys) when the noise filter is set to off. If the noise filter is set to fine, medium or coarse then peaks and valleys which deviate from the preceding peak or valley by a percentage of the total excursion of the scan (the difference between the highest peak and the lowest valley) are omitted from the statistcal peaks and valleys tables.
  • Page 20 Performing a Scan It is recommended that a new baseline scan is carried out prior to performing spectrum scans. Select BASELINE from the menu options at the bottom of the display using the left arrow key and then press . A baseline scan will now be executed. Select SCAN from the menu options at the bottom of the display using the right arrow key.
  • Page 21 Cursor The menu option allows a movable cursor to be displayed on the scan. The absorbance or transmittance at the selected wavelength is displayed. The cursor can be moved to scanned wavelengths using the left and right or Goto keys. Selecting the cursor menu option changes the displayed menu option to MENU.

  • Page 22: Multi-Wavelength Mode

    3.5 MULTI-WAVELENGTH MODE Having selected the Multi-Wavelength Mode from the Main Menu options the following display will be shown: Mode Takes the instrument back to the operating mode menu. Set-up Allows access to the multi-wavelength mode set-up menu and instrument set-up menu. Sample Initiates a sample measurement sequence.
  • Page 23 Instrument Set-up... Allows access to the instrument set up menu. Accessory Set-up... Only displayed if an accessory is fitted. It is recommended that set-up parameters be reviewed prior to calibration or measurement to ensure the selected measurement mode and values are correct. Select SET UP mode using the key and press Performing a Measurement...

  • Page 24: Kinetics Mode

    3.6 KINETICS MODE Having selected Kinetics Mode from the Main Menu options the following display will be shown: Kinetics Pre-Measurement Menu Mode Takes the instrument back to the operating mode menu. Set-up Allows access to the kinetics mode set up menu and instrument set-up menu. Start Begins a kinetics run.
  • Page 25 Factor This value is multiplied by the average absorbance or transmittance changing rate to give the enzyme value (EV). Record Range... Allows access to the kinetics record range menu option which allows the Y-Axis range to be set. Y-Axis Max The highest absorbance or transmittance (depending on the measurement mode) that is displayed on the kinetics axis.
  • Page 26 Start Level... Allows access to the start kinetics run on level or after lag time set-up menu. Start On Level If set to on, then the instrument will wait until the measured absorbance or transmittance reaches the user set start level before recording kinetics run time readings when a kinetics measurement is started. If set to off, then the instrument will wait for the user set lag time before taking run time readings when a kinetics measurement is started.
  • Page 27 The instrument is now ready to perform a kinetics measurement. Place the unknown sample into the sample chamber and close the lid. Move the cursor to START using the arrow keys and press to confirm. The instrument display will then show a blank graph with the option to ABORT the scan.

  • Page 28: Quantitation Mode

    3.7 QUANTITATION MODE This menu can only be accessed when using the optional multi-cell holder. Mode Takes the instrument back to the operating mode menu. Set-up Allows access to the quantitation mode set-up menu and instrument set-up menu. Calibrate Constructs a quantitation calibration curve. The current settings for the standard concentrations and the number of standards are used.
  • Page 29 Quantitation Set-up Menu Measure Mode Selects between transmittance or absorbance display on the quantitation graph Y-Axis. Units Selects the concentration units of measurement. Storing Warning Messages When a calibration curve is saved with two calibration curves already saved, the instrument prompts to confirm the new save, since the oldest saved calibration curve will be overwritten.
  • Page 30 Record Range... Allows access to the quantitation record range menu option which allows the Y-Axis range to be set. Y-Axis Max. The highest absorbance or transmittance (depending on the measurement mode) that is displayed on the calibration curve Y-Axis. Y-Axis Min The lowest absorbance or transmittance (depending on the measurement mode) that is displayed on the calibration curve Y-Axis.

  • Page 31: Good Practice Guidelines

    3.8 GOOD PRACTICE GUIDELINES For optimum performance blank and sample calibration should be carried out at the beginning and end of every sample batch. To ensure accurate results are obtained the sample area lid should be kept in the closed position during measurement.

  • Page 32: Maintenance

    MAINTENANCE 4.1 GENERAL The 6400/6405 have been designed to give optimum performance with minimal maintenance. It is only essential to keep the external surfaces clean and free from dust. The sample area lid should be kept clean and accidental spillage should be wiped away immediately.

  • Page 33: Deuterium Lamp Replacement

    Refit the lamp access panel to the rear of the unit, taking care not to trap the earthing cable in the casework. Secure into position using the 4 fixing screws. 4.3 DEUTERIUM LAMP REPLACEMENT (MODEL 6405) The deuterium lamp has an extended life of 2000 hours. Should the lamp need to be replaced the...
  • Page 34 Disconnect the unit from the mains supply. Access to the deuterium lamp can be gained via the lamp access panel located at the rear of the unit. Lamp replacement should be carried out by removing the 4 slot head screws from the panel. NOTE: The panel is attached to the unit case via a short earthing cable.

  • Page 35: Accessories

    035 025 Visible Flow-through Cell 1.8ml 035 045 Visible Flow-through Cell 80µl 035 044 UV Flow-through Cell 1.8ml (6405) 035 047 UV Flow-through Cell 80µl (6405) 643 001 (Peltier) Heated Cell System European 230V - comprising heated cell block and external power supply module.

  • Page 36: Spares

    Plastic 100mm path length Cell - Pack 100 060 229 Plastic standard Cuvettes (3.5ml) - Pack of 500 060 230 UV Plastic standard Cuvettes (3.5ml) - Pack of 100 (6405) 060 232 UV Plastic standard Cuvettes (3.5ml) - Pack of 500 (6405) 060 087 Plastic semi-micro Cuvettes (1.6ml) - Pack of 100...

  • Page 37: Interfacing

    The 25 way D connector allows a standard one-to-one interconnection lead to be used. The RS232 serial interface allows data to be read from the 6400/6405 by sending simple ASCII character commands. Similarly, this interface can also be used to remotely operate the basic functions of the instrument.
  • Page 38 Table 2 - Remote Control Mode Serial Commands (<cr> is ASCII carriage return) Command Serial Response Notes Action none R<cr> Switches the instrument into remote control mode. Enter remote This command is only accepted when the control mode instrument is displaying the operating mode menu. Instrument display will indicate remote control mode enabled.

  • Page 39: Rs232

    - LINKED TO RTS DSR 6 - OUTPUT FROM 6400/6405 DCD 8 - OUTPUT FROM 6400/6405 DTR 20 - INPUT TO 6400/6405 (must be active) GND 7 Suggested interconnections are detailed below: 6400/6405 IBM PC XT (25 way “D”) TXD 2...

  • Page 40: Recorder Output

    6400/6405 IBM PC XT (9 way “D”) TXD 2 TXD (From PC) RXD 3 RXD (To PC) RTS 4 RTS (From PC) CTS 5 CTS (To PC) DSR 6 DSR (To PC) DCD 8 DCD (To PC) DTR 20 DTR (From PC)
  • Page 41 SECTION 8 WARNING AND ERROR MESSAGES a) Messages relating to Power On Self Test Self Test Failure This warning occurs after the power-on system tests have been performed. The system test check boxes will indicate which test has failed (this may or may not allow the instrument to be used, depending on the failure detected, see below).
  • Page 42 This error can also occur if the lamp has failed, or, in the case of the 6405, if the deuterium lamp has failed to strike or is in the process of warming up (deuterium lamp symbol flashing).

  • Page 43: Photometrics Mode

    c) Photometrics Mode Concentration Factor Too High This error is reported when the resulting multiplication factor required for a photometrics mode concentration calibration is calculated to be outside of the instrument limits. d) Spectrum Mode No Spectrum Data Available This warning occurs when an action which processes the data from a scanned spectrum is performed. For example, pressing the print key with only a blank scan graph will report this warning rather than printing the blank axis.

  • Page 44: Kinetics Mode

    Warning - Existing Data Will Be Overwritten This warning message is displayed when the user attempts to save a spectrum scan or quantitation calibration curve and previous data has already been stored. The instrument can store two scans and quantitation calibration curves, but must over write the oldest stored data if a subsequent scan or calibration curve is stored.
  • Page 45 Insufficient Data to Construct a Calibration Curve This warning message is displayed if the user aborts a quantitation calibration before two or more calibration points have been performed. The instrument cannot construct a calibration curve with less than two calibration points so will display a blank axis. Insufficient Data to Save Calibration Method This warning message is displayed if the user aborts a quantitation calibration and then attempts to save the aborted calibration.
  • Page 46 SECTION 7 EXAMPLE PRINTOUTS a) Photometrics Mode Note: Cell holder position will only be given when the multi-cell holder is fitted. ======================================== 6400 SPECTROPHOTOMETER PHOTOMETRICS MODE 15:14:29 19/11/97 INSTRUMENT ID NUMBER: 6400 OPERATOR........ SAMPLE ID....... LAST CALIBRATED: 15:13:59 19/11/97 WAVELENGTH: 345.0nm ======================================== 100.0%T 345.0nm 15:14:33 19/11/97...
  • Page 47 b) Spectrum Mode ======================================== 6400 SPECTROPHOTOMETER SPECTRUM MODE 16:00:03 19/11/97 INSTRUMENT ID NUMBER: 6400 OPERATOR........ SAMPLE ID....... START WAVELENGTH: 600.0nm END WAVELENGTH: 700.0nm SCAN INTERVAL: 5.0nm SAMPLE NUMBER: CELL HOLDER POSITION: LAST BASE LINE 14:51:56 19/11/97 ======================================== nm Abs 600.0, 0.297 605.0, 0.313 610.0, 0.332 615.0, 0.359...
  • Page 48 c) Multi-Wavelength Mode ======================================== 6400 SPECTROPHOTOMETER MULTI WAVELENGTH 16:02:21 19/11/97 INSTRUMENT ID NUMBER: 6400 OPERATOR........ SAMPLE ID....... LAST CALIBRATED: 16:02:16 19/11/97 WAVELENGTH 1: 500.0nm WAVELENGTH 2: 600.0nm ======================================== 16:02:24 19/11/97 W1 500.0nm 1.417Abs W2 600.0nm 1.551Abs A1 - A2 = -0.134Abs A1 / A2 = 0.913 CELL HOLDER POSITION:...
  • Page 49 d) Kinetics Mode ======================================== 6400 SPECTROPHOTOMETER KINETICS MODE 16:04:22 19/11/97 INSTRUMENT ID NUMBER: 6400 OPERATOR........ SAMPLE ID....... CELL HOLDER POSITION: LAST CALIBRATED: 11:12:26 14/11/97 WAVELENGTH: 600.0nm FACTOR: 100.000 RUN TIME(SEC): 10 SEC ======================================== TIME 0, 1.874 1, 1.870 2, 1.867 3, 1.852 4, 1.842 5, 1.824...
  • Page 50 e) Quantitation Mode ======================================== 6400 SPECTROPHOTOMETER QUANTITATION MODE 16:06:52 19/11/97 INSTRUMENT ID NUMBER: 6400 OPERATOR........ SAMPLE ID....... LAST CALIBRATED: 16:05:33 19/11/97 WAVELENGTH: 635.0nm STANDARDS USED : 5 CAL STANDARD 1 : 10.000 74.8%T CAL STANDARD 2 : 20.000 66.3%T CAL STANDARD 3 : 30.000 49.1%T CAL STANDARD 4 : 40.000 28.1%T CAL STANDARD 5 : 50.000 5.4%T...
  • Page 51 EC Declaration of Conformity Jenway Models 6400 and 6405 Spectrophotometers comply with the following European Standards: EN 50081-1:1992 Electromagnetic compatibility - Generic emission standard EN 50082-1:1992 Electromagnetic compatibility - Generic immunity standard (Performance criterion B) EN 61010-1:1993 Safety requirements for electrical equipment for measurement, control and...
  • Page 52 Thank you for reading this data sheet. For pricing or for further information, please contact us at our UK Office, using the details below. UK Office Keison Products, P.O. Box 2124, Chelmsford, Essex, CM1 3UP, England. Tel: +44 (0)330 088 0560 Fax: +44 (0)1245 808399 Email: sales@keison.co.uk...

This manual is also suitable for:

6400

Table of Contents