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Summary of Contents for jenway 6305
- Page 1 Model 6305 Spectrophotometer Operating Manual 635 015 REV B/06-14...
- Page 3 1. The 63 series spectrophotometers are designed for operation by trained personnel who are aware of the principles and applications involved. For further help and advice please visit www.jenway.com 2. The spectrophotometer is a sensitive electronic and optical instrument designed for use in a laboratory environment.
- Page 4 über die entsprechenden Prinzipien und Anwendungen Bescheid weiß, vorgesehen. Weitere Hilfe und Hinweise finden Sie auf www.jenway-equipment.de 2. Ein Spektrophotometer ist ein empfindliches elektronisches und optisches Instrument, das für die Verwendung in Laboren vorgesehen ist. Die Installationsanweisungen sind genau zu beachten.
- Page 5 Si necesita más ayuda o indicaciones, diríjase a www.jenway.com 2. El espectrómetro es un instrumento electrónico y óptico de gran sensibilidad destinado a entornos de laboratorio. Deben seguirse atentamente las instrucciones de instalación. En caso de duda, consulte a la autoridad competente y pertinente antes de continuar.
- Page 6 Per ulteriori informazioni e per assistenza visitare il sito internet www.jenway.com 2. Lo spettrofotometro è uno strumento ottico elettronico sensibile progettato per l'uso in laboratorio. Seguire minuziosamente le istruzioni di installazione. In caso di dubbi rivolgersi all'autorità...
- Page 7 7. Fare sempre riferimento ai dati sulla salute e la sicurezza per tutte le sostanze chimiche o reagenti utilizzati. Osservare attentamente tutte le informazioni, i consigli e le avvertenze disponibili sulla loro manipolazione, conservazione, utilizzo e smaltimento. Qualora tali dati non siano disponibili, richiederli al fornitore prima di procedere in qualsiasi modo.
- Page 8 Contents SECTION 1 - Introduction ..................9 INSTRUMENT DESCRIPTION..................... 9 INSTRUMENT SPECIFICATION..................9 SECTION 2 – Installation ..................10 UNPACKING........................10 INSTALLATION ........................10 DISPLAY ..........................11 CONTROLS ........................11 REAR PANEL ........................12 SAMPLING ......................... 13 SECTION 3 – Theory and Practice of Spectroscopy Measurements ....... 14 THEORY OF SPECTROSCOPY MEASUREMENT ............
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Page 9: Section 1 - Introduction
SECTION 1 - Introduction INSTRUMENT DESCRIPTION The 6305 spectrophotometer is suited to a wide range of applications in education, quality control, environmental and clinical analysis. This model is a UV/visible spectrophotometer covering a wavelength range from 198nm to 1000nm, with measurement modes for absorbance, % transmittance and concentration. -
Page 10: Section 2 – Installation
(HH180(S)), or US mains lead moulded plug (FCABLEUS) 3. Pack of 100 disposable plastic visible wavelength cuvettes (060 084) 4. Jenway 63-zero PC software (635 100) and interface cable (013 210) model 6300 only 5. Instruction manual (635 015) 6. -
Page 11: Display
The instrument will automatically re-align the monochromator using spectral peaks associated with the xenon flash lamp. During this initialisation, the instrument displays CAL on the primary display before displaying the main measurement screen. Please note that the instrument will go to the last wavelength and measurement mode used. -
Page 12: Rear Panel
Fig. 2.4.1 – Controls 1. Arrow Keys: Used to adjust values on the selected display 2. Arrow Keys: Used to move the cursor horizontally between menu options 3. Return Key: Used to select the display menu option 4. CAL Key: Initiates a calibration routine 5. -
Page 13: Sampling
3. Power in socket IEC type connection socket for mains cable 4. Output sockets Analogue output 5. RS232 serial port Connection to a PC SAMPLING Model 6305 is supplied with a 10mm cuvette holder. Other accessories can be fitted (refer to section... -
Page 14: Section 3 – Theory And Practice Of Spectroscopy Measurements
SECTION 3 – Theory and Practice of Spectroscopy Measurements THEORY OF SPECTROSCOPY MEASUREMENT UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample. -
Page 15: Spectroscopy Measurement
The optical layout of the 6305 spectrophotometer is shown below: Figure 3.2.1 – Diagram of light path The light from the xenon lamp is focused onto the grating, with 1200 lines per millimeter, which separates the light into discreet wavelengths. -
Page 16: Good Practice Guidelines
GOOD PRACTICE GUIDELINES 1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambient temperature and light levels should remain as constant as possible. 2. If required adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP) should be monitored with regular calibration checks and a suitable Quality Control (QC) programme. - Page 17 10. Cuvettes and sample holders must be filled to a minimum level which covers the light path. All Jenway spectrophotometers have a beam height of 15mm. 11. The instrument must be calibrated to zero absorbance/100% transmittance prior to taking...
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Page 18: Section 4 – Photometrics Measurements
SECTION 4 – PHOTOMETRICS MEASUREMENTS The 6305 can perform simple measurements of % transmittance or absorbance. The sample is measured at one wavelength and at one point in time. TRANSMISSION MEASUREMENT MODE To select % Transmittance use the LEFT or RIGHT arrow keys to move the cursor to the %T indicator on the menu bar (refer to figure 2.3.1). - Page 19 measured.
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Page 20: Section 5 – Concentration Measurements
SECTION 5 – CONCENTRATION MEASUREMENTS The concentration measurement mode enables simple measurements of concentration to be performed. In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor. The sample is measured at one wavelength at one point in time. To select concentration use the LEFT or RIGHT arrow keys to move the cursor to the CONC indicator on the menu bar (refer to figure 2.3.1). -
Page 21: Section 6 – Connecting To A Pc
A printout is initiated by pressing the PRINT key. If the sample number is unity, then the printout will include a header block. The sample number is incremented every time the PRINT key is pressed. The following commands can also be sent to the 6305 via the serial interface (using Windows HyperTerminal, for example):... -
Page 22: Rs232 Output
– LINKED TO RTS CTS 5 – OUTPUT FROM 6305 DSR 6 – OUTPUT FROM 6305 DCD 8 – INPUT TO 6305 (must be active) DTR 20 GND 7 Suggested interconnections are detailed below: IBM PC XT (25 way “D”) 6305... -
Page 23: Recorder Output
IBM PC XT (9 way “D”) 6305 TDX 2 3 TXD (From PC) RXD 3 2 RXD (To PC) RTS 4 7 RTS (From PC) CTS 5 8 CTS (To PC) DSR 6 6 DSR (To PC) DCD 8 1 DCD (To PC) -
Page 24: Section 7 – Accessories And Spare Parts
Interface cable kit 7.2 SPARES Part Code Description of Spare Part 012 094 Xenon Flash Lamp Module (6305) 630 204 10x10mm path length cuvette holder 632 511 Dual cell holder for 10mm cuvettes and 12.7mm diameter tubes (6320D ONLY) 060 084... -
Page 25: Section 8 – Maintenance And Service
SECTION 8 – Maintenance and Service ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust. The sample area should always be kept clean and any accidental spillage should be wiped away immediately. To give added protection when not in use, the unit should be disconnected from the mains supply and covered with the optional dust cover. -
Page 27: Service
Decontamination Certificate. This is available as a downloadable pdf file at www.jenway.com, or contact us and we will be happy to send you a copy. Please clearly mark the package for the attention of the Service Department and post to the following address:... -
Page 28: Section 9 – Troubleshooting
SECTION 9 – Troubleshooting 9.1 ERROR CODES Refer to the table below to diagnose error codes. If you require further assistance contact Jenway Help (refer to section 9.2). Error Code Error Name Issue Err 1 Dark cal error This error occurs when the 6305 closes the shutter to block light entering the sample chamber. -
Page 29: Section 10 – Declaration Of Conformity
SECTION 10 – Declaration of Conformity... - Page 30 Bibby Scientific Ltd Bibby Scientific France SAS Bibby Scientific Ltd. – Singapore Beacon Road Stone ZI du Rocher Vert - BP 79 Prudential Tower, Level 26, Staffordshire ST15 0SA 77793 Nemours Cedex 30 Cecil Street 049712 United Kingdom France Singapore Tel: +44 (0)1785 812121 Tel: +33 1 64 45 13 13 Tel: +65 6631 2976...
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Questions and answers
Hello I'm currently using the Jenway 6305 in my laboratory. Since today, the spectrophotomer is not working anymore : we can turn it on, but then the display is blocked on the Transmittance (%T) mode, with no possibilities to change to Abs. What sould we do? Thanks in advance for you answer, Lucie Thibaud, Reasercher.
Use the LEFT or RIGHT arrow keys to move the cursor to the absorbance (Abs) indicator on the menu bar. If the issue persists, ensure the instrument is properly calibrated to zero absorbance/100% transmittance before taking readings. If the problem continues, refer to troubleshooting guidelines or contact support.
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