Prepare To Acquire Data - Agilent Technologies 6460 Manual

Before you begin

Prepare to acquire data

Prepare to acquire data
If you do not intend to acquire data but want to learn how to use the
Qualitative Analysis program for method development, you can skip this step,
which tells you how to prepare the demo sample. You then do those tasks that
show you how to use the Qualitative Analysis program with the sulfa drug
data files shipped with the system.
Parts List
The exercise in this guide uses this equipment and materials:
Table 1
Triple Quadrupole
6460
1 Prepare the LC solvents.
2 Prepare the sample.
3 Set up the LC column.
4
• Agilent K1260 Infinity LC modules: well-plate sampler, binary pump,
thermostatted column compartment, DAD
• Zorbax column (see
• A 1 ng/μL concentration of the sulfa mix sample (prepared in this step)
Zorbax column
Column Description
RRHD Eclipse Plus
C18.2.1 mm x 50 mm
For the A channel, add 1 mL of 5M ammonium formate to a 1-liter reservoir
filled with HPLC-grade water.
For the B channel, add 1 mL of 5M ammonium formate to a 1-liter reservoir
filled with 90:10 acetonitrile and HPLC-grade water.
a Add 10 μL of the sulfa mix from one of the ampoules (500 μL) to 990 μL
of solvent A in a 2 mL glass sample vial so that the final concentration is
1 ng/μL.
b Cap the vial and place in a sample location in the autosampler.
Use the column from
may be used in these exercises, but some parameters may need adjustment,
and the results will differ.
Table 1 on page 4)
Particle
Size
1.8 µm
Table 1. Other columns and instrument parameters
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Pore Size Part
Number
95Å 959757-902