Task 3. Create A Dynamic Mrm Method Using Update Dmrm - Agilent Technologies 6460 Manual

Exercise 2 – Develop a Dynamic MRM method from an MRM acquisition data file or an MRM method

Task 3. Create a Dynamic MRM method using Update dMRM

Task 3. Create a Dynamic MRM method using Update dMRM
You can create a Dynamic MRM method from an MRM data file or a
Quantitative Analysis method. You first set the Scan Type to Dynamic MRM,
and then you use the Update MRM Method dialog box.
Steps
1 Open the method iiiSulfamix
MRM_10.m and save it to a new
name with the format iiiSulfamix
dMRM.m, where iii are your
initials.
2 Change the method to a dynamic
MRM method with the same
compounds. You can either use a
data file or the report that was
generated in the last task.
38
Detailed Instructions
a Click File > Open > Method.
b Select the iiiSulfamix MRM_10.m
method. Click OK.
c Click Method > Save As.
d Type the new method name with the
format iiiSulfamix_dMRM.m.
a Click the Acquisition tab in the QQQ
tab in the Method Editor window.
b Right-click the Scan segments table
and click Update DMRM Method. The
Dynamic MRM Update Options dialog
box opens.
c Select the folder containing the
report.results.xml file or the data file
iiiSulfamix MRM_10.d.
d Mark Add new compound/transition.
e Mark Update retention time.
f Mark Update trigger window.
g Click OK.
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Comments
• In this example, the batch is in the
\MassHunter\Data\
MRM_to_DMRM folder.
• The Dynamic MRM Update tool
automatically sets the Scan type to
Dynamic MRM.
• You can select either a data file that
was acquired with a Scan Type of
MRM or a Quant Report folder as
the input to this dialog box. The
Scan segments are created from
one of these two input sources.
You can update the compounds in the Scan
segments table by using a QQQ data file or a
Quantitative analysis report folder.
If you select a Quantitative analysis report
folder, you need to make sure to generate the
results file. See "Task 2. Print a report in the
Quantitative Analysis program"
learn how to always generate the results file.
on page 36 to